Yoga Nidra for hypertension: A systematic review and meta-analysis

Background Hypertension is a prevalent chronic condition that affects a substantial proportion of the world's population. Medications are commonly prescribed for hypertension management, but non-pharmacological interventions like yoga are gaining popularity. Objective The purpose of this systematic review and meta-analysis is to assess the efficacy of Yoga Nidra (YN) for the management of hypertension. Methods A systematic review and meta-analysis of clinical trials, i.e., non-randomized and randomized controlled trials (RCTs) was conducted to investigate the effects of YN on hypertension. PubMed, the Cochrane Library, SCOPUS, and EBSCO were searched for relevant studies published up to September 2022. The quality of the included studies was assessed using the Cochrane risk of bias tool. The primary outcome measure was the change in systolic blood pressure (SBP) and diastolic blood pressure (DBP) after YN intervention, analyzed as weighted mean difference (WMD), in comparison to control groups. The random-effects model was used for the meta-analysis. Risk of bias was assessed for RCTs and non-RCTs using Cochrane's RoB-2 and ROBINS-I tools, respectively. Results Five RCTs and three Non-RCTs involving a total of 482 participants (239 for YN vs 243 for controls) were included in this review. The meta-analysis indicated that YN significantly reduced SBP (WMD = 12.03 mm Hg, 95% CI [7.12, 16.93], Z = 4.80, p < 0.00001) and DBP (WMD = 6.32 mm Hg, 95% CI [3.53, 9.12], Z = 4.43, p < 0.00001) compared to control groups. The overall risk of bias for the three RCTs was high, whereas for the five non-RCTs, one had an overall moderate risk while the other four had an overall serious risk of bias. Discussion This systematic review and meta-analysis provides evidence supporting the efficacy of YN as a complementary therapy for hypertension management. YN is a safe, cost-effective, and easily accessible intervention that primarily relies on interoception and induces a deep relaxation response in practitioners, aiding them in coping with various components of high blood pressure, such as stress, vascular inflammation, peripheral vascular resistance, etc. Our understanding of the mechanisms of YN is constantly evolving, and there is a need for further research to fully explore and appreciate the significance of this ancient science and its potential efficacy on BP. Considering the results and the multifactorial role of YN, it can act as a safe and reliable adjuvant therapy to complement the pharmacological treatment of hypertension. However, further studies with larger sample sizes, longer follow-up periods, and homogenous populations are warranted. Conclusion This meta-analysis suggests that YN is effective in reducing SBP and DBP, particularly in individuals with hypertension. The results highlight the potential of YN as a complementary therapy for hypertension management. Healthcare providers may consider recommending YN to patients with hypertension as an adjuvant therapy to medication. Further studies are required to identify standardized optimal forms and durations of YN best suited for hypertension management.

therapy to medication.Further studies are required to identify standardized optimal forms and durations of YN best suited for hypertension management.

Introduction
Hypertension, also known as high blood pressure (BP), is defined by the American Heart Association (AHA) as a chronic medical condition characterized by consistently elevated BP, with systolic blood pressure (SBP) of more than 130 mm Hg or diastolic blood pressure (DBP) of more than 80 mm Hg [1].This increased pressure puts significant strain on the heart and blood vessels, raising the possibility of serious complications, affecting over a billion people worldwide [2].In India, the overall prevalence of hypertension is 29.8%, with 27.6% in rural populations and 33.8% in urban [3].Hypertension accounts for approximately 54% of all strokes and 47% of coronary artery diseases, thus causing more deaths than any other cardiovascular risk factors [4].The global burden of disease study ranks elevated SBP as the leading metabolic risk factor attributable to disability adjusted life years [5], while the overall number of cardiovascular deaths continues to rise as societies age [6].
There are two broad categories of hypertension: essential hypertension, which affects 85% of all patients without an identifiable cause; and secondary hypertension, which is caused by underlying conditions such as renal artery stenosis, pheochromocytoma, adrenal adenoma or single gene mutations.Investigating the pathophysiology of hypertension is still a work in progress as several mechanisms, including increased sympathetic drive, renin-angiotensin-aldosterone system activation, receptor-mediated vasoconstriction or impaired vasodilation, increased reactive oxygen species, genetic influences, and several other immunemediated mechanisms, all work together to contribute to hypertension [7,8].Understanding these mechanisms has aided in the development of hypertension-specific therapies.
As per the AHA, management of hypertension in all its stages involves pharmacological and non-pharmacological interventions [1].Weight loss, low sodium intake, physical activity, and moderation in alcohol consumption are the main non pharmacological interventions used in the management of hypertension [1].Pharmacological interventions include categories of drugs like calcium channel blockers, angiotensin converting enzyme inhibitors, angiotensin receptor blockers, beta blockers, diuretics and sympatholytic drugs.Anti-hypertensive medications have a crucial role in hypertensive emergencies and urgencies.Despite their proven efficacy, high drug costs and drug interactions pose a significant barrier in patient's adherence to medication.Long-term use of certain drugs can result in disabling side effects such as nausea, muscle cramps, dizziness, dry mouth, and so on [9,10].Moreover, long-term use of antihypertensive medication has been linked to a lower quality of life and financial constraints [12].This adds to mental stress and poor mental health of the hypertensive patient [11].Discontinuation of the prescribed drug reverts the patient's pathophysiology, resulting in high BP and contributing to high rates of poorly controlled hypertension [10].Therefore, in chronic management of hypertension, antihypertensive drugs can work in unison with non-pharmacological interventions for a better patient outcome.Thus, lifestyle changes should be used to prevent or treat hypertension, with or without medication.Although dietary changes and exercise are beneficial, they rarely manage hypertension on a long-term basis due to low patient motivation and effort [13].Given the shortcomings of currently available strategies, it is prudent to investigate newer, more cost-effective, and easier-to-administer therapies to combat the ever-growing morbidity of hypertension that may convey the benefits of long-term adherence [8].
Yoga is a complementary and alternative therapeutic modality that originated in India and consists of physical postures, breathing practices, relaxation exercises and meditation techniques [14].Yoga practice is suggested to lower BP by lowering stress, increasing parasympathetic activation and changing baroreceptor sensitivity [14,15].Previous research suggests that yoga is an effective adjunct therapy for hypertension management.The AHA scientific statement from 2017 states that meditation techniques like the Transcendental Meditation can possibly benefit on cardiovascular risk and can be considered for treating individuals with BP above normal thresholds [16].However, due to the wide variety of yoga practices and the variable quality of the research, it is difficult to recommend any specific yoga practices [15].
Amongst the multitude of yoga techniques, Yoga Nidra (YN) is a supine relaxation technique performed in Shavasana (corpse pose) [17,18] and is sometimes referred to as "yogic sleep" [18,19].It appears similar to hypnosis, but YN differs to the effect that the practitioner retains his free will and is mentally aware of his surroundings and himself [18,19].Yoga masters describe the mental state in YN as a relaxed yet fully aware state of consciousness, in which the mind is neither asleep nor awake [19] and has been equated to the hypnagogic state of consciousness i.e., the transitioning phase from wakefulness to sleep [17].The Ashtanga Yoga of Maharishi Patanjali advocates "Pratyahara," or withdrawing the mind and mental awareness from the outwardly manifested sensory channels.Pratyahara is the primary modality used by YN to internalize awareness and induce interoception [17].YN practices also use techniques such as body/breath awareness, progressive muscular relaxation, and guided imagery to lead the practitioner's mind through a specific series of mental images and sensations, distinguishing it from other relaxation techniques such as meditation or mindfulness [17,18].All of this work together to activate the relaxation response in the body which results in autonomic changes and BP regulation.YN has been shown to have a variety of health benefits including stress and anxiety reduction, improved sleep and overall well-being, and is quickly becoming popular among the general public due to its broad applicability, safety, and feasibility [18,20].Growing research backs the use of YN in the management of hypertension, and there is ever-expanding evidence in favor of YN [20].
Despite numerous investigational studies, there is no conclusive pooled evidence on the effect of YN on hypertension and BP.This study aims to conduct a comprehensive literature review to qualitatively and quantitatively synthesize the anti-hypertensive effects of YN among studies assessing healthy and diseased adults, with mean BP thresholds exceeding normal values (SBP >120 mm of Hg and DBP >80 mm of Hg).This pooled effect may help determine whether YN can be included as an evidence-based adjunct therapy for hypertensive patients that is lowcost, feasible, and easy to implement with minimal contraindications.

Search strategy and sources
A systematic literature search was performed in the electronic databases of PubMed, Scopus, Cochrane Library, and EBSCO Essentials for eligible studies published until September 20, 2022.Relevant keywords, including hypertension, blood pressure, prehypertension, mean arterial pressure, systolic blood pressure, diastolic blood pressure, SBP, DBP, elevated blood pressure, yoga nidra, yoganidra, yoga-nidra, shavasana, psychic sleep, corpse pose, yogic relaxation, yogic sleep, yogic rest, supine yoga relaxation, and guided sleep meditation, were used for searching literature using Boolean operators "AND" and "OR".All searches were restricted to titles, abstracts and keywords of the respective databases and limited to English language only.Searches were re-run by the authors before analysis to check for any discrepancies.
N. Ahuja et al.

Eligibility criteria
Only experimental and quasi-experimental studies i.e., randomized controlled trials (RCTs), non-RCTs and clinical trials having at least two comparable population groups were selected based on their eligibility for the following criteria framed in PICO format (Population Intervention Comparator Outcome).

Selection process
Search results from different databases were compiled in a single excel file and checked for duplicates using Rayyan, an internet-based application for systematic reviews [22].After removing duplicates, two co-authors independently screened the remaining studies for potential reports worth inclusion using a screening checklist form created on the online platform "Google Sheets" to be filled out simultaneously.Prior to selection, each study's abstract was thoroughly assessed to ensure its eligibility based on the PICO format of the original review question.During the screening procedure, any conflicts that arose were discussed with a third co-author and resolved by consensus.After the screening process was complete, the authors double-checked every selected study for any errors.

Data extraction
Three review authors independently extracted data from studies on basic characteristics (e.g., author, year, country, study design, etc.), participant details (e.g., demographics, age, gender, comorbidities, etc.), intervention details (e.g., allocation ratio, nature, length, and duration of intervention, dropouts, etc.), outcome data and analysis (assessment time points, pre and post intervention results, statistical tests used, etc.) and other details (e.g., protocol registration, participant's informed consent, ethical clearance, conflict of interest, etc.).Data extracted on the above points was recorded using google sheets, based on the data collection form for intervention reviews (RCTs and non-RCTs) by Cochrane.

Risk of Bias Assessment
Included studies were assessed for risk of bias using the Revised Cochrane Risk-of-Bias Tool for Randomized Trials (ROB-2) and Risk-of-Bias in Non-Randomized Studies of Interventions (ROBINS-I) tool, as per their respective study designs.Assessments were performed independently by three co-authors and judgments regarding the risk of bias were made after consensus and under scrutiny of a senior co-author.

Quantitative assessment
Cochrane's RevMan software (Version 5.4) was used to conduct a meta-analysis and generate the forest plot.For statistical analysis, the mean differences and standard deviations of the differences between the intervention groups and the control groups were sought from the data provided within the selected manuscripts.The calculations were performed using the Cochrane handbook's provided formulas.The I 2 statistic was used to test for heterogeneity, with I 2 values above 50% indicating significant heterogeneity.Tau 2 (τ 2 ) was used to determine the proportion of heterogeneity explained by subgroup differences.

Protocol registration
The review protocol was registered prospectively with the International Prospective Register of Systematic Reviews (PROSPERO) on January 02, 2022, under registration number CRD42022283667.

Study characteristics
The sample sizes for the studies ranged from 30 to 150.The mean age of participants in most of the studies was between 40 and 60 years old, except for one study that had a mean age of under 30 years in each group [44].Thangam et al. [51] enrolled elderly participants with both stage and 2 hypertension, Anjana et al. [46] included stage 1 hypertensives, while Patel et al. [48,50], Devraj et al. [49] and Deepa et al. [47] included Stage 2 hypertensives.Participants without hypertension were included in the two studies by Monika et al. [44] and Lijun Li et al. [45], which focused on females with menstrual irregularities and patients undergoing colonoscopy, respectively.However, the participants in both studies had elevated baseline BP (SBP >120 or DBP >80 mm of Hg), so both studies were taken into consideration for our review.Three studies had patients who were on anti-hypertensive drugs [46][47][48], two studies included patients of which some were on antihypertensive agents [49,50], two studies did not mention the use of antihypertensive agents [45,51], and one study had subjects with elevated BP, not on any medication [44].The duration of YN intervention ranged from 15 to 40 min except for one study that did not define the duration [50].Two of the included articles assessed the effects of YN along with standard pharmacological medications versus standard medication alone [44,51].Two studies by Patel et al. [48,50] have used psychophysical relaxation based on yogic principles, reinforced by biofeedback instruments, along with pharmacological medications.Their intervention meets our defined criteria for YN, and compares for self-relaxation and standard medications among age and gender matched controls.The biofeedback instrument provided patients with continuous feedback about their SNS activity through an auditory signal, allowing for deeper relaxation while their BP levels were monitored throughout the yogic relaxation sessions.Four studies used other co-interventions in addition to YN, such as Om chanting [46,47], a brisk walk [47,49], joints rotation [47], and music therapy [45].However, YN accounted for more than 50% of the time in each study's experimental group intervention, making it a crucial behavioral component of the overall intervention.The duration of follow-up ranged from 15 days to 12 months, with an average of three months in most studies.Table 1 details the characteristics of individual studies.

Risk of Bias Assessment
The following sections elaborate on the Risk of Bias for the three RCTs and five non-RCTs, assessed using Cochrane's ROB-2 and ROBINS-I tools, respectively.

Assessments for RCTs using ROB-2
For the first domain of bias due to the randomization, two of the three RCTs had some concerns as there were observable differences in outcomes at baseline [46] and a lack of clarity about the method of randomization [48].Under domain two, i.e., bias due to deviations from intended interventions, one study had high risk due to a significant  number of people dropping out with no specific reasons mentioned [44], while another study had some concerns due to a lack of intention to treat analysis [46].For domain three of bias due to missing outcome data, one study had high risk due to non-disclosure of outcome data of drop-outs and no proper justification for the drop-outs [46].There was a low risk for the fourth domain, i.e., bias due to measurement of outcomes, since all the three included studies utilized BP and other objective measures, measured at parallel time points for both experimental and control groups.For the fifth domain of bias due to selective reporting of the results, some concerns were observed for two studies due to the lack of a pre-trial protocol and statistical analysis plan [46,48], while high risk was observed for one study for reporting comparisons between outcomes measured at different time-points [44].All of the above add up to a high overall risk of bias for the cumulative results of all three randomized controlled trials taken together.Fig. 2(a) depicts the risk of bias assessed for individual RCTs, while Fig. 2(b) depicts the percentage of risk of bias assessed for different domains of ROB-2 tool.

Assessments for non-RCTs using ROBINS-I
Out of the five non-RCTs, one had an overall moderate risk [48], rest all four had an overall serious risk of bias [45,47,49,51].Since hypertension can be influenced by confounding factors such as age, gender, diet, lifestyle, comorbidities, etc. the bias due to initial confounding is moderate for two studies [47,50] and serious for three studies [45,49,51] mostly due to a lack of adequately matched controls.Under the domain of bias due to deviation from intended intervention, four studies had no information available on prior protocols and statistical analysis plans [45,47,49,51], while the fifth study [50] had moderate risk.Except for one study which had no information on the missing outcome data [47], all the five studies had a low risk for the remaining five domains of bias i.e., selection of participants, classifications of interventions, missing data, measurements of outcomes and selective reporting of results.Fig. 3(a) depicts the risk of bias assessed for individual non-RCTs while Fig. 3(b) depicts the percentage of risk of bias assessed for different domains of ROBINS-I.

Meta-analysis
In three studies [44,45,47], the standard deviations of the difference in BP were not provided.Hence, the imputation method described in the Cochrane document was used [52].One of the studies [48] was chosen to calculate the correlation coefficient because this study provided both the difference in means as well as the standard deviation between preand post-intervention in both the experimental and control groups and had a relatively consistent correlation value for all the outcomes.The overall effect (Z) favors YN as an intervention over the control, with the direction of the effect, for both SBP and DBP, favoring YN (overall effect for SBP: Z = 4.80 [p = 0.00001]; overall effect for DBP: Z = 4.43 [p = 0.00001]).The heterogeneity is very high for both SBP (τ 2 = 39.08;χ 2 = 95.81,df = 7 [p < 0.00001]; I 2 = 93%) and DBP (τ 2 = 12.88; χ 2 = 67.82,df = 7 [p < 0.00001]; I 2 = 90%).Table 2(a) and 2(b) show the statistical computations of the meta-analysis of all the included RCTs and non-RCTs, for both SBP and DBP, respectively.Fig. 4(a) and (b) depict the forest plots of all the RCTs and non-RCTs for both SBP and DBP, respectively.

Discussion
Hypertension is a complex, multifactorial disease with numerous interrelated mechanisms contributing to its pathophysiology.The autonomic nervous system (ANS) and the central nervous system (CNS) play crucial roles in regulating BP.Sympathetic overactivity and increased sympathetic tone, coupled with decreased parasympathetic activity, contribute to hypertension [53].Apart from the above, various other biomechanisms that may lead to the development of HTN include oxidative stress [54], insulin and leptin resistance [55,56], reduced baroreceptor sensitivity [57], poor circadian rhythm [58], mental stress, anxiety and depression [59,60], excessive anti-natriuretic factors such as aldosterone and endothelin-1 [61], diet and sodium intake [62], genetic factors [63], microbiome with gut dysbiosis [64], Renal dysfunction [65], and finally, vascular inflammation [66] driven by numerous factors such as oxidative stress, inflammation, and endothelial dysfunction, which also contribute to hypertension's development.
The impact of yoga on various physiological systems has been documented, and several mechanisms have been proposed regarding its beneficial role in hypertension.Yoga has a positive impact on the ANS because it successfully decreases SNS activity while simultaneously increasing PNS activity [67].Yoga has been shown to reduce stress levels [68] and oxidative stress markers, such as malondialdehyde, while simultaneously increasing the presence of antioxidant enzymes, such as superoxide dismutase [69].Yoga has also demonstrated the ability to enhance insulin sensitivity and mitigate leptin resistance [

71]
, improve baroreceptor sensitivity and autonomic tone [72,73], and normalize the circadian rhythm by decreasing the levels of cortisol and elevating the levels of melatonin [74,75].All of the above aid in the reduction of BP and a consequent enhancement in the individual's cardiovascular well-being.
There is ample evidence supporting how yoga alleviates symptoms of mental stress, anxiety, and depression by enhancing autonomic functions, activating neurohormonal processes, and suppressing sympathetic activity [73,76].Incorporating yoga into one's lifestyle can even lead to improved dietary habits and a reduction in sodium intake [77,78], both of which have been associated with a decrease in BP.While the impact of yoga on genetics is yet to be fully explored, previous research indicates that these practices may have the ability to regulate gene expression and enhance cardiovascular health [79][80][81].They also help improve the interactions of the gut-brain axis by fine-tuning the modulation of the gut microbiota through the CNS and ANS pathways [82,83].This decreases inflammation, leading to potential indirect benefits for BP regulation.Studies also show that regular yoga practice can enhance renal function and mitigate proteinuria, thereby leading to a potential reduction in BP [84,85].Yoga effectively decreases the presence of inflammatory markers, including C-reactive protein [86,87], leading to enhanced cardiovascular health and positively impacting BP.
Different yoga practices like asana, pranayama, meditation, YN, etc. have their own deep and intricate mechanisms in the body-mind complex, whose investigation is a vast topic in itself.The effect of YN on HTN can be attributed to its mechanism of primarily initiating a relaxation response, which reduces sympathetic activity and enhances parasympathetic activity.Thus, YN operates primarily on the brain by inducing relaxation throughout the neural system [19,88,89].By lowering sympathetic overactivity and moderating heart rate, YN helps reduce peripheral vascular resistance and BP [21,89].In addition, by addressing the mental health of hypertensive individuals, such as anxiety, depression, and stress, it has a secondary benefit in the reduction of BP [21].Similar to meditation, YN might also help reduce vascular inflammation and, hence, have positive effects on BP [90].Since YN does not involve physical postures or asanas and can be practiced in a comfortable supine position, it can prove to be an ideal practice for the elderly, who are at a higher risk of developing hypertension and may have difficulty exercising and following an active lifestyle [91].Thus, YN can be a potential therapeutic modality for hypertension.However, there is a paucity of research on YN [21,88,89]; for example, the effect of YN on the underlying mechanisms of HTN can be explored, and further   research is required to comprehensively study the potential mechanisms of YN on BP and hypertension.
Our results on YN are consistent with previous review studies [ [92][93][94][95] assessing the effects of other yoga interventions on BP.They have reported favorable effects of yoga on hypertension, and the current evidence points to positive outcomes for YN as a therapeutic modality.However, we need to further explore the evidence and mechanisms so that the practices of yoga and YN in particular may be adapted more in clinics as well as introduced in the therapeutic guidelines for hypertension once substantial evidence is generated.Also, it is to be noted that yoga comprised different practices in almost all of the studies.We should utilize the common mechanisms and goals behind these and try to minimize the specific techniques and approaches that vary widely depending upon the tradition, teacher, and individual practitioner [17][18][19].Hence, there is a need to quantify the practices of YN to better understand its therapeutic potential [18,20].YN has been shown to have a wide range of physical, mental, and emotional benefits, and it is important to understand how these benefits can be maximized in different contexts.For future studies, specific ways to standardize and quantify the practices should be utilized to better understand their therapeutic potential.For example, using specific YN protocols, standardized outcome measures, and consistent training and certification standards can help assess the effects of YN on different health outcomes more accurately and make evidence-based recommendations for its use in clinical settings.
The strengths of our study are that, to the best of our knowledge, this is the first meta-analysis to assess the effects of YN on BP.The limitations of the study are that the overall risk of bias for all five non-RCTs was reported to be serious, while the overall risk of bias for two out of three RCTs was reported to be high.While it is difficult to control for individual differences in intervention trials, bias due to confounding variables such as age, gender, diet, lifestyle, comorbidities, etc. can be minimized by following a robust RCT study design or by carefully matching participants.Research conducted among highly homogenous populations, such as military personnel, the elderly at old age homes, prison inmates, etc., might help to minimize heterogeneity and confounding as participants in these scenarios adhere to a common dietary regimen, circadian cycle, and lifestyle [96].
Studies in the current review lacked participant blinding, while assessor blinding was still implemented in some studies since there is a practical problem of blinding participants regarding the intervention provided in the case of yoga interventions.The authors propose that special scoring criteria and bias evaluation instruments such as the "Risk of Bias Justification Table " (RATIONALE) could be utilized and  Fig. 4(a).Forest plot of all the included RCTs and non-RCTs for systolic blood pressure.

Fig. 4(b).
Forest plot of all the included RCTs and non-RCTs for diastolic blood pressure.
N. Ahuja et al. developed in order to improve the design and evaluation of behavioral intervention studies such as yoga [97].Another limitation is that the studies differed in the treatments delivered to the subjects; apart from YN, some trial participants took antihypertensive medications while others did not.This resulted in the heterogeneity of our results.
To add to the methodological rigor and improve the strength of the evidence, future researchers should keep a few pointers in mind while conducting RCTs, such as having a well-defined pre-trial protocol, a statistical analysis plan, considering a homogeneous population with a large sample size, using standardized interventions, having active comparator control groups, using standardized and validated outcome measures, adhering to planned timepoints, conducting longer followups, and using intention-to-treat analysis in line with the protocol [96].Following appropriate reporting guidelines like the "Consolidated Standards of Reporting Trials" (CONSORT) [98], or the "CheckList stAndardising the Reporting of Interventions For Yoga" (CLARIFY) guidelines in case of Yoga based studies, is equally important for the meticulous and transparent dissemination of the research findings [99].
Our chosen topic of review had a limited availability of good-quality studies, which led to a relatively small number of studies being included.This may have resulted in a narrower scope of analysis and reduced statistical power, which could have affected the generalizability and validity of the review's conclusions.Due to the scarcity of good-quality studies, this review had to include studies with different designs, settings, and populations, which added to the heterogeneity, making it more challenging to draw meaningful conclusions from the pooled data.Furthermore, the lack of high-quality studies may have led to a potential for publication bias and an overestimation of the size of the effect of the intervention being studied.Lastly, a number of factors-including small sample sizes, methodological flaws, and brief follow-up periods-may have reduced the quality of the studies included in this review.These factors could have affected the accuracy and generalizability of the study's findings.Despite these challenges, there are several reasons why there is a need to generate more interest in conducting trials on YN.YN provides distinct advantages for individuals with HTN, addressing the physical, mental, and neurological dimensions simultaneously.YN has the capacity to promote profound relaxation, diminish stress, and enhance parasympathetic activity, which is one of the mechanisms through which it reduces HTN.Additionally, its low-cost and non-invasive nature, along with its simplicity in terms of instructions, demonstration, accessibility, and adaptability, add to YN's potential to benefit a wide range of populations, including those who may not be able to perform physical exercise or yoga asana or pranayama.
As mentioned earlier, there is a growing body of research suggesting that YN itself may be an effective adjunct therapy for many health conditions.As such, there is a need to better understand how this practice can be tailored to different populations and conditions in order to maximize its therapeutic potential.Finally, there is a need to generate more interest in YN research in order to increase public awareness of the benefits of YN and promote its integration into mainstream healthcare settings.By conducting high-quality scientific studies on YN, further evidence can be generated to support its use in clinical practice and help to overcome some of the skepticism that still exists around complementary and alternative therapies.

Conclusion
A significant overall effect was observed in favor of YN for a sample size of 682 participants (239 experimental group vs. 243 controls), with an overall reduction as Mean Difference of 12.03 (7.12 ± 16.93) mm Hg for SBP and 6.32 (3.53 ± 9.12) mm Hg for DBP.The current review establishes a strong basis for YN to be used as an alternative and complementary treatment modality for managing hypertension, however, there is a dearth of research evidence available till date and further trials are needed to generalize these findings.With the growing use of internet, audio-visual and mobile based applications, YN might prove to be an attractive, cost effective, easily accessible, simple and safe treatment modality for the major worldwide health issue of Hypertension.Additional studies on YN should be conducted with a heightened understanding of the risk for bias and trial quality assessments while integrating additional tools and techniques for quantifying the psychological, neurological, endocrinological, immunological effects of YN on BP and hypertension.High-quality RCTs with rigorous methodology are needed to investigate standardized and validated YN programs as treatment and preventive modalities for hypertension, not only as a supplemental therapy but also as a primary intervention.Future studies should focus on larger sample sizes, longer follow-up periods, and more rigorous study designs to provide more robust evidence on the effectiveness of YN for hypertension management.This could include randomized controlled trials that compare the effects of YN to other therapies, as well as studies that investigate the underlying mechanisms of action.

Sources of funding
None.

Fig. 2 .
Fig. 2. (a) Risk of bias assessment for randomized controlled trials (RCTs).(b) Risk of bias assessment for Randomized Controlled Trials (RCTs) depicted as percentage for the domains of ROB-2 tool.

Fig. 3 .
Fig. 3. (a) Risk of bias assessment for non-randomized controlled trials (Non-RCTs).(b) Risk of bias assessment for Non-Randomized Controlled Trials (Non-RCTs) depicted as percentage for the domains of ROBINS-I tool.

Table 1
Characteristics of included studies (continued on next page) N.Ahuja et al.

Table 2 (b)
Statistical Computations of the Meta-analysis of all the included RCTs and non-RCTs for the outcome of Diastolic Blood Pressure.