Creatine and low-dose lithium supplementation separately alter energy expenditure, body mass, and adipose metabolism for the promotion of thermogenesis

Summary Nutraceutical approaches to promote adipose tissue thermogenesis may help to prevent obesity onset. Creatine is a critical regulator of adipose metabolic function and low-dose lithium supplementation has been shown to promote adipose thermogenesis. In the present study, we sought to directly compare the two supplements for their effects on adipose metabolism and thermogenesis. We show that both supplements increase daily energy expenditure (EE) and reduce body mass in male Sprague-Dawley rats. Lithium increased brown adipose tissue (BAT) mitochondrial and lipolytic proteins that are associated with thermogenesis, while creatine increased BAT UCP1 and mitochondrial respiration. The BAT thermogenic findings were not observed in females. White adipose tissue and skeletal muscle markers of thermogenesis were unaltered with the supplements. Together, the data show that low-dose lithium and creatine have diverging effects on markers of BAT thermogenesis and that each increase daily EE and lower body mass in a sex-dependent manner.


INTRODUCTION
Adipose tissue is classically thought to function as a storehouse for excess energy but has since been recognized for its potential to dissipate energy in the form of heat under mechanisms conserved to combat cold challenges.The process of energy dissipation for heat production in adipose tissue is referred to as nonshivering thermogenesis and in certain circumstances can account for up to 15% of daily energy expenditure in humans. 1,2Promoting the energy expending potential of nonshivering thermogenesis at rest is therefore a valuable target for obesity therapeutics and prophylactics as obesity rates are projected to rise considerably in the coming years. 3ithium supplementation in low doses, resulting in serum concentrations far below the typical therapeutic window for manic disorders (0.5-1.2 mM), has been shown to improve the functional or characteristic markers of many metabolic diseases. 4,5Recently, Geromella et al. demonstrated that low dose lithium supplementation (10 mg/kg/day, resulting in a serum Li concentration of only 0.02 mM 6 ) increased daily energy expenditure and promoted a beiging phenotype in inguinal white adipose (iWAT) 7 in male mice.Lithium has been shown to potently inhibit GSK3 8 and it is thought that the thermogenesis-inducing properties of lithium are mediated through its inhibition of GSK3.Previous work has uncovered that selective GSK3b inhibition promotes the expression of thermogenesis-associated genes in brown adipocytes in vitro. 9urthermore, it is known that GSK3 activity within adipose tissue is 2-fold higher in diet-induced obese mice 10 and its inhibition can reduce pro-inflammatory M1 macrophages 11 and improve insulin sensitivity 12 and signaling. 13The previous evidence adds support to the hypothesis that low-dose lithium supplementation can induce positive metabolic outcomes and promote adipose thermogenesis, potentially through its inhibition of GSK3.
Separately, creatine is emerging as a critical regulator of adipose metabolism and thermogenesis.5][16][17][18] However, FCC has only been shown to be activated with adrenergic stimuli which questions its clinical translatability as potent adrenergic stimuli poses many risks for people with obesity. 19To our knowledge, it is unknown if creatine supplementation alone can increase markers of adipose thermogenesis at rest.A recent paper has reported that Ucp1 gene expression was increased with creatine-supplementation in the brown adipose tissue (BAT) of high fat fed (HFD)-fed mice. 20In humans, creatine supplementation was unable to increase BAT activity via positron emission tomography (PET) scan 21 although the dosing of creatine spanned only seven days.Twenty-eight days of creatine supplementation was found to increase resting metabolic rate in humans 22 but it was not studied if BAT was a contributor to the increase in basal metabolism.Separately, a retrospective study was able to correlate creatinine clearance with BAT activity via PET scan, 23 which also correlates creatine abundance with thermogenic capacity.Animal studies provide the best evidence for the necessity of available creatine to adipose thermogenic function.Animals treated with a creatine analog that impairs creatine metabolism were found to have less UCP1 in their BAT depot. 24In addition, mice that lack the ability to endogenously synthesize creatine within their adipose depots have a reduced whole-body VO 2 that is recovered by creatine supplementation. 15Lastly, work from our lab has observed that creatine supplementation results in higher levels of key mitochondrial markers in adipose across sexes and depots. 25The aforementioned supports the hypothesis that creatine availability is a regulator of adipose metabolism and has the potential to influence thermogenic capacity.
The promotion of thermogenesis at rest may be a vital tool to combatting the development of obesity at the population level.The previous evidence suggests that supplemental lithium and creatine could be an effective method of promoting thermogenesis without the risk that comes with administering potent adrenergic stimuli.The aim of the present study is to investigate if lithium and creatine, supplemented separately or concurrently, can increase whole-body energy expenditure (EE) and adipose tissue markers of metabolism and thermogenesis.Importantly, the study also seeks to determine if the proposed thermogenic effects of lithium and creatine supplementation translate to females as all the previously cited evidence in support of lithium and creatine supplementation for promoting adipose thermogenesis was conducted solely in male models.This research will provide evidence for the utility of minimally invasive supplements, when utilized in a chronic manner, to effect energy expenditure and thermogenic markers in adipose tissue.It will also provide sex and depot specific comparisons to determine, which are most affected by the supplementation.

RESULTS
Lithium and creatine supplementation resulted in higher energy expenditure and lower body mass in males but not females In males, EE was higher than control in all treatment groups (Figure 1A).The higher EE with Li cannot be attributed to differences in locomotion as total meters traveled within the metabolic caging unit over 48 h were not altered with supplementation (Figure 1B).Similarly, Cr did not increase meters traveled within the caging unit but was approaching significance (p = 0.0561; Figure 1B).
Final body mass was lower than control in Li and Li+Cr and approached significance in Cr (p = 0.0618; Figure 1C).Reductions in body mass are not attributable to reduced food intake as there were no observed differences between groups (Figure 1D).
In females, there was no effect of treatment on EE (Figure 1E).Despite a similar EE, Li animals were found to travel more within their cages compared to all other groups (Figure 1F).There were no effects of treatment on body mass (Figure 1G) or average daily food intake in the females (Figure 1H).Creatine increases UCP1, and mitochondrial respiration in male BAT and lithium increase BAT mitochondrial and lipolytic proteins in males but not females To determine if the changes in EE and body mass were a result of increased adipose thermogenesis, thermogenic, mitochondrial, and lipolytic proteins were investigated in BAT.Mitochondrial density, measured as cardiolipin concentration, 26 was not different between experimental groups in either males (Figure 2A) or females (Figure 2D).Cr but not Li increased protein content of primary thermogenic markers UCP1, and cytochrome c which approached significance (p = 0.0653) in males (Figure 2B) with no effect of treatment in females (Figure 2E).Li but not Cr increased PGC1a protein content, a marker of mitochondrial biogenesis, in males but not females (Figures 2B and 2E).Markers of electron transport chain proteins were differentially influenced by the treatments as Complex V was increased in males (Figure 2C) and decreased in females (Figure 2F) with Li treatment.Cr increased Complex I and total protein content of the five complexes of the electron transport chain in females (Figure 2F) but not males (Figure 2C).
To further examine the potential mechanisms mediating the higher EE in male rats, BAT mitochondrial respiration was examined.No changes were observed in Complex II respiration between treatments (Figures 3A and 3B), Complex IV respiration was higher in Cr-and Li+Cr-treated male BAT (Figures 3A and 3C).Li treatment alone did not affect Complex IV respiration.
Li but not Cr increased lipolytic markers including total hormone sensitive lipase (HSL), phospho-S563 HSL, phospho-S660 HSL (Figure 4A), as well as phospho-S406 ATGL in males (Figure 4B).The ratio of phosphorylated-to-total HSL S660 approached significance (p = 0.080) and the ratio of phosphorylated-to-total ATGL was higher with lithium (Figures 4A and 4B).Conversely, in females, Li and Li+Cr had lower total HSL and phospho-S660 HSL protein whereas Li alone lowered phospho-S563 HSL and phospho-S406 ATGL (Figures 4C and 4D).Li-treated groups also had lower phosphorylated-to-total HSL S563 and S660 ratios as well as the phosphorylated-to-total ATGL ratio in the BAT of females.

Lithium and creatine supplementation alters WAT adipocyte area
To determine if the treatments shifted WAT morphology toward a beiging phenotype and if this may also contribute to the increased energy expenditure, iWAT was analyzed by H&E stain to assess the presence of multilocular adipocytes and the adipocyte area.There were no visible browning/beiging in either WAT depot between the sexes (Figures 5A and 5F).Despite the lack of overt browning, we decided to examine adipocyte area.In male WAT there was an approaching significant relative frequency shift toward more smaller adipocyte areas in the Cr group (p = 0.0622; Figure 5B).In females, Lithium resulted in less adipocytes in the 1000-4000 mm 2 range and more adipocytes in the 0-1000 mm 2 range although the latter only approached significance (p = 0.0584; Figures 5G and 5H).
To further examine the effects of the supplements on adipose thermogenesis, markers of thermogenesis were examined in WAT.Both Li and Cr had no effect on UCP1, PGC1a or cytochrome c protein content in the WAT of either sex (Figures 6A and 6C).At the level of the electron transport chain, Li decreased protein content of Complex V in males (Figure 6B) whereas Li and Cr independently decreased Complex I and II in females (Figure 6D).

WAT lipolytic markers are lower with lithium-supplementation in both sexes
Finally, Li decreased lipolytic proteins including total HSL, phospho-S660 HSL, phospho-S563 HSL, and total ATGL in males as well as the phosphorylated-to-total HSL S660 ratio (Figures 7A and 7B).The lithium effects on lipolytic markers continued to phospho-S563 HSL and phospho-S660 HSL in females being lower with lithium (Figure 7C) with no effect of the treatments on total or phosphorylated ATGL protein content (Figure 7D).In contrast, creatine-treated groups were found to have higher phospho-S660 HSL in males and higher phosphorylatedto-total HSL S660 ratio (Figures 7A and 7C).Lithium increased GSK3b Ser9 inhibitory phosphorylation in BAT in males but not females with major sex differences in BAT GSK3 content Li increased total and phospho-S9 GSK3b in BAT of males (Figure 8A) whereas total GSK3b in females was lower with Li and had no effect on GSK3b S9 phosphorylation (Figure 8C).In iWAT, Li decreased phospho-Ser9 GSK3b in males (Figure 8B).In contrast, Li and Cr increased total GSK3b with no change in phosphorylated GSK3b in females (Figure 8D).Lastly, it was found that total GSK3 protein content was $20-fold higher in female BAT compared to males (Figure 8E) and may help to explain the sexually dimorphic effects of lithium on markers of BAT metabolism.

DISCUSSION
Alterations to brown adipose metabolism may underlie the sex specific whole-body effects of the supplements It is important to study the chronic administration of nutritional supplements for their utility in promoting thermogenesis as a prophylactic measure to combat the rising rates of obesity in the developed world. 27This study sought to compare the adipose depot effects of two nutritional supplements, creatine and lithium, which we have previously shown to exert effects on adipose thermogenesis.We demonstrate that lithium and creatine supplementation similarly increase EE and lower body weight in male rats consistent with what would be seen with increased nonshivering thermogenesis.We then identified alterations in thermogenic, mitochondrial, and lipolytic markers in BAT with minimal effects on skeletal muscle and WAT markers of thermogenesis.This would suggest that both creatine and lithium exert their thermogenic effects mostly via alterations to BAT but diverge in the markers altered by each individual supplement.Creatine was found to increase Complex IV respiration and UCP1 and cytochrome c protein content in male BAT.In concordance with this finding, a recent study has shown an increased Ucp1 gene expression with creatine supplementation in rodents. 20Furthermore, it was found in a model of chronic creatine depletion that UCP1 protein expression is reduced following beta-guanidinopropionic acid treatment, an inhibitor of cellular creatine uptake. 24Our Complex IV respiration and cytochrome c protein expression findings, taken together with UCP1 expression, are suggestive of thermogenic mitochondrial uncoupling in male BAT following creatine treatment.Increased electron transport chain activity likely results from UCP1 uncoupling, with futile respiratory activity contributing to increased energy expenditure observed in male mice.
Lithium increased markers of lipolysis in the BAT of males, along with PGC1a and Complex V protein expression.It is the GSK3 inhibition by lithium that we believe to be a key to the effects of lithium on BAT.It is known that inhibitory phosphorylation of GSK3 can upregulate mitochondrial function. 28PGC1a was found to be higher with lithium supplementation and could be secondary to lithium-mediated GSK3-inibition. 29,30There were, however, no changes in cardiolipin content suggesting the higher PGC1a content did not induce mitochondrial biogenesis in this model.It is known that GSK3 regulates mitochondrial functions that go beyond biogenesis and could still be mediated by PGC1a. 28It is also possible that the higher PGC1a that was observed with lithium supplementation could be a result of the higher lipolytic markers in the depot as the activation of lipolysis results in increased activity of transcription factors involved in thermogenic remodeling, including PGC1a. 31Total and S563 and S660 phosphorylated HSL and phosphorylated ATGL were higher in lithium supplemented groups, which was also true of the ratios of phosphorylated-to-total protein content for both proteins in male BAT.The opposite was observed in female BAT and aligns well with the data suggesting that GSK3 is more inhibited at its Ser9 site in male BAT but not female BAT.
Together, the data suggest that creatine and lithium supplementation both exert effects on BAT thermogenic markers, however, the markers that are altered diverge between the supplements.Creatine was found to increase more typical UCP1-dependent nonshivering thermogenesis at the BAT, whereas lithium increased markers of lipolysis which could suggest a GSK3-lipolysis induced mechanism of thermogenesis that is not present in creatine-treated animals.As there were no synergistic effects of the supplements, it is likely that these separate mechanisms of thermogenesis cannot be concurrently activated.It has been shown previously that UCP1-dependent thermogenesis will be active at the expense of UCP1-independent mechanisms 14 but more research into this topic field is needed before such a hypothesis can be applied to the present work.It will be important in the future to determine dose-response curves for the supplements to determine optimal supplementation paradigms based on circulating concentrations and are a limitation of the present study.

Creatine and lithium exert sex-specific effects on markers of thermogenesis
Our lab has previously shown that a similar creatine supplementation protocol increases creatine content in the BAT of males with no difference in females. 25This difference in males and females could underlie the sex-specificity of the present creatine-induced UCP1 and energy expenditure findings.A sex-dimorphism in the effects of creatine supplementation have been repeatedly observed and requires further examination. 32,33One possible underlying mechanism behind the sex difference in creatine effect is that females have 10% greater resting intramuscular creatine stores. 34Since brown adipocytes and myocytes are of the same developmental origin, it is possible that female BAT has greater resting creatine stores and therefore supplementation would have a lesser effect on intracellular supply but requires further examination and the utilization of estrogen-depleted animal models to determine the estrogen-dependence of this observation.An important difference that may underlie the sex specificity of the lithium results is the GSK3b response to supplementation.In male BAT, lithium supplementation resulted in higher GSK3b phosphorylation at its Ser9 inhibitory site, as initially hypothesized, and this effect is absent in females.The cause of the sex-specific effect of lithium supplementation is unknown but one possibility is that the females have higher GSK3b protein content and since the kinase is constitutively active, it may be harder for a low dose of lithium to have a strong inhibitory effect on GSK3b.
WAT and skeletal muscle do not take on thermogenic characteristics with the supplements WAT can express functionally thermogenic proteins that are upregulated in a process known as browning/beiging and contribute to daily EE. 35We therefore examined the influence of lithium and creatine on WAT morphology and thermogenic protein expression.There were minimal effects of both treatments on WAT adipocyte area across sexes including no observed multilocularity and therefore no overt adipocyte browning/beiging effect.There were minor changes observed in ETC proteins and were independent of any changes in the primary thermogenic proteins UCP1 and PGC1a.Lipolytic markers were also lower in WAT of lithium treated groups in both sexes.Collectively, these results suggest that the treatments are specific to BAT for their promotion of thermogenic markers.And contrasts with the study by Geromella et al. 36 which observed a consistent increase in mitochondrial proteins, including UCP1, and multilocular phenotype in WAT with low-dose lithium supplementation.The difference in results is likely due to model differences as the present study was done in rats compared to a mouse model used in the previous study.The two studies are similar, however, in that it is the depot in which GSK3b is more inhibited by lithium that will result in changes to thermogenesis associated proteins.
Skeletal muscle is also a contributor to nonshivering thermogenesis and has the potential to increase daily energy expenditure, 37,38 therefore we also included examination of skeletal muscle nonshivering thermogenesis.Skeletal muscle nonshivering thermogenesis is thought to function primarily through the inefficient transport of calcium at the sarco(endo)plasmic reticulum Ca 2+ ATPase (SERCA). 39,403][44] Low-dose lithium supplementation has been shown previously to increase SERCA uncoupling in C2C12 myoblasts and in the soleus of chow and HFD-fed mice.Similar uncoupling and upregulation of SERCA uncouplers was observed in muscle-specific GSK3 knockdown mice. 36In the present study we examined SERCA coupling along with the protein expression of known uncouplers and contributors to SERCA-dependent thermogenesis including GSK3, RYR, SLN, and NNAT.None of the previous markers of muscle nonshivering thermogenesis were altered with lithium supplementation (Figure S1) and may be dissimilar to previous results due to the utilization of a different rodent model and dosing than was used previously.The changes in energy expenditure with the treatments is therefore likely coming from the adipose tissue.
Increasing thermogenic activity at rest with nutritional supplements might be a particularly useful prophylactic tool to mitigate the likelihood of obesity development as present prophylactic obesity tools have proven either dangerous or ineffective.The results of the present study highlight the potential utility of low-dose lithium and creatine supplementation to impact EE, body mass, and markers of adipose thermogenesis, however, this was primarily in the BAT depot and there was no synergy detected between the two supplements, highlighting distinct and separate modes of action.Future work could test the effects of increasing the dose of lithium especially given that our study is limited in that we could not measure serum Li levels.Furthermore, as the thermogenic effects of these two supplements were only found in male rats, it would be of great importance to determine why the results varied between male and female rats as it will pave a path toward making these supplements effective for thermogenesis in both sexes.In this respect, focusing on the effects of sex hormones and their possible interplay with these supplements would likely shed valuable insight.

Limitations of the study
The present study was limited in that the serum concentrations of lithium and creatine were not recorded.However, it has been previously shown that the same creatine supplementation protocol used in the present study results in increased adipose tissue creatine content suggesting that a suitable creatine supplementation protocol was selected. 25Similarly, the serum concentration of lithium in the present study is unknown but is expected to be $0.1 mM based on previous literature, which is far below typical therapeutic values of $1.2 mM 45 and is similar to studies that show thermogenic potential for lithium. 36Furthermore it is unknown if the sex-specificity of the findings are estrogen-dependent or are resultant of another mechanism.It is of critical importance to determine how to overcome sex-dependent mechanistic differences to promote optimal supplementation protocols for both sexes.Lastly, the supplements were provided ad libitum to the animals in their water  supply and therefore the amounts of the supplements consumed between animals is variable.Natural variability in dosing can result in the inability to capture minute effects in outcome variables, however, many robust changes were observed despite the variability in dosing, which can be interpreted as strong effects of the supplements that persist despite the variability in dosing.

Mitochondrial respiration in frozen tissue
Methods for measuring mitochondrial respiration in frozen BAT using Seahorse analysis were adapted from previously published methods papers. 48,49For each biological sample, 10mg of brown adipose tissue was thawed on ice and transferred to a pre-chilled small volume glass-to-glass Dounce homogenizer.Samples were homogenized using 500 uL ice-cold 1 x MAS buffer (70 mM sucrose, 220 mM mannitol, 5 mM KH2PO4, 5 mM MgCl2, 1 mM EGTA, 2 mM HEPES pH 7.4) per 10mg sample, and twenty manual strokes of the tissue homogenizer.
Homogenates were centrifuged at 2000 x g for 3 minutes at 4 C and the supernatant collected.Protein concentration was determined using a BCA.Using the BCA protein concentrations, homogenates were diluted in MAS buffer to normalize samples to 3 ug sample protein per well in 60uL of sample (0.05ug/uL).Homogenate dilutions were then loaded in triplicate per biological sample to a pre-warmed Seahorse XFe24 microplate and centrifuged at 2,000 x g for 5 min using a plate centrifuge (low break).390uL of pre-warmed 1 x MAS buffer (pH 7.4) supplemented with 10ug/mL (final concentration) was then gently added to each well.The plate was immediately loaded into a XFe24 Analyzer precalibrated with XFe24 cartridge sensors.Substrate injections (final concentrations) for the measurement of respiratory capacity through complex II and IV were as follows: Port A) 5mM succinate and 2uM rotenone, Port B) 4uM Antimycin A, Port C) 0.5mM TMPD and 1mM ascorbic acid, Port D) 50mM sodium azide.Following each injection, oxygen consumption rate (OCR) was measured for 4 minutes two times, with a 30 second mix period allowed before each measurement period.OCR data was exported from the Wave software (Agilent) with no further normalization required due to the normalization of samples to 3ug protein prior to loading.All OCR were thus exported as pmol/min/3ug protein.Complex II-and IV-dependent respiration was calculated by subtracting OCR following complex inhibitor injection from OCR following substrate injection.

Cardiolipin concentration analysis with HPTLC
Total lipids were extracted from 50ug of frozen BAT tissue as previously described. 50The lipid extract was spotted onto high-performance thin layer chromatography plates (HPTLC; 5633-5, EMD Chemicals, Darmstadt, Germany) and neutral lipids were separated from phospholipids using hexane/diethyl ether/acetic acid (70:30:1, by vol.) as a solvent 51 after allowing the solvent to run up each plate for 30 min.The phospholipid band was scrapped from the HPTLC plate, lipid extracted again, and spotted onto another HPTLC plate, this time using chloroform:methanol:acetic acid:water (100:75:7:4, by vol.) as a solvent system to separate individual phospholipids, 52 allowing the solvent to run up each plate for 30 min.The plates were then charred at 180 C after saturation with a 50% aqueous sulfuric acid solution.Cardiolipin was quantified by obtaining densitometric images using the BioRad ChemiDoc touch imaging system (CAT# 1708370) and quantification performed on AlphaView (ProteinSimple) software.

Statistical analysis
Comparisons across treatments were performed via two-way ANOVA presented as mean G SEM with all measurements being made relative to the control groups.Post-hoc analysis was completed with Fischer's multiple comparisons test.Statistical significance was assumed at p % 0.05.GraphPad Prism 8 software (GraphPad Software, La Jolla, CA, USA) was used to perform all statistical analyses.* denotes significantly different from control P< 0.05, ** P<0.01, *** P<0.001, **** P<0.0001, P value written above a straight horizontal line is denoting a main effect of its associated treatment.

Figure 1 .
Figure 1.Whole-body parameters (A) Male energy expenditure recorded every 30 min over a 24-h period (left figure) and then averaged over 24-h (right figure) (B) Male total meters traveled in the metabolic caging system as recorded by X, Y, Z beam breaks.(C) Final male body mass in kg.(D)Male food intake was recorded three times weekly and then averaged over the 6-week supplementary period.(E-H) the same measures as A-D but for females.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.

FFigure 2 .
Figure 2. BAT mitochondrial and thermogenic proteins (A) cardiolipin concentration as measured by HPTLC in males.(B) Western blotting quantification for UCP1, PGC1a, and cytochrome c in males.(C) Western blotting quantification for OXPHOS antibody cocktail in males.(D) cardiolipin concentration as measured by HPTLC in females.(E) Western blotting quantification for UCP1, PGC1a, and cytochrome c in females.(F) Western blotting quantification for OXPHOS antibody cocktail in females.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.

Figure 3 .
Figure 3. BAT mitochondrial respiration in males (A) Oxygen consumption rate (OCR) over time (B) Complex II respiration (C) Complex IV respiration.p value written above a straight horizontal line is denoting a main effect of its associated treatment.Data are presented as mean G SEM.

Figure 4 .
Figure 4. BAT lipolytic proteins (A) Total and phosphorylated HSL (Ser563, Ser660) western blot quantifications for males as well as the ratio of phosphorylated/total protein content for the two above phosphorylation sites (B) Total and phosphorylated ATGL (Ser406) western blot quantifications for males as well as the phosphorylated/total ratio.(C) Total and phosphorylated HSL (Ser563, Ser660) western blot quantifications for females as well as the ratio of phosphorylated/total protein content for the two above phosphorylation sites.(D) Total and phosphorylated ATGL (Ser406) western blot quantifications for females as well as the phosphorylated/total ratio.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.

Figure 5 .
Figure 5. WAT histological characterization (A) H&E-stained adipocytes were quantified for adipocyte area and then put into a histogram for males.(B-E) Adipocytes were grouped into four separate categories: 0-1000 mm 2 , 1100-4000 mm 2 , 4100-7000 mm 2 and >7000 mm 2 for males.(F) H&E-stained adipocytes were quantified for adipocyte area and then put into a histogram for females.(G-J) Adipocytes were grouped into four separate categories: 0-1000 mm 2 , 1100-4000 mm 2 , 4100-7000 mm 2 and >7000 mm 2 for females.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.The scale bar on the representative histological images denotes 100mm.

Figure 6 .
Figure 6.WAT mitochondrial and thermogenic proteins (A) Western blotting quantification for UCP1, PGC1a and cytochrome c in males.(B) Western blotting quantification for OXPHOS antibody cocktail in males (C) western blotting quantification for UCP1, PGC1a cytochrome c in females (D) western blotting quantification for OXPHOS antibody cocktail in females.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.

Figure 7 .
Figure 7. BAT lipolytic proteins (A) Total and phosphorylated HSL (Ser563, Ser660) western blot quantifications for males as well as the ratio of phosphorylated/total protein content for the two above phosphorylation sites.(B) Total and phosphorylated ATGL (Ser406) western blot quantifications for males as well as the phosphorylated/total ratio.(C) Total and phosphorylated HSL (Ser563, Ser660) western blot quantifications for females as well as the ratio of phosphorylated/total protein content for the two above phosphorylation sites.(D) Total and phosphorylated ATGL (Ser406) western blot quantifications for females as well as the phosphorylated/total ratio.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.

Figure 8 .
Figure 8. GSK3b content and inhibitory phosphorylation in BAT and iWAT (A) Total and Ser9 phosphorylated GSK3b western blot quantification in male BAT.(B) Total and Ser9 phosphorylated GSK3b western blot quantification in male WAT.(C) Total and Ser9 phosphorylated GSK3b western blot quantification in female BAT.(D) Total and Ser9 phosphorylated GSK3b western blot quantification in female iWAT.(E)Total GSK3b protein content relative to total protein content equalized across male and female brown and white adipose tissue to compare relative protein expressions across tissues.Data were analyzed by two-way ANOVA and are presented as mean G SEM, * denotes significantly different from control p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, p value written above a straight horizontal line is denoting a main effect of its associated treatment.