Quorum sensing signals improve the power performance and chlortetracycline degradation efficiency of mixed-culture electroactive biofilms

Summary Electroactive biofilms (EABs) play an important role in bioelectrochemical systems due to their abilities to generate electrons and perform extracellular electron transfer (EET). Here, we investigated the effects of quorum sensing (QS) signals on power output, chlortetracycline degradation, and structure of EABs in MFCs treating antibiotic wastewater. The voltage output of MFCs with C4-HSL and PQS increased by 21.57% and 13.73%, respectively, compared with that without QS signals. The chlortetracycline degradation efficiency in closed-circuit MFCs with C4-HSL and PQS increased by 56.53% and 50.04%, respectively, which resulted from the thicker biofilms, higher biomass, and stronger activities. Additionally, QS signals induced the heterogeneous distribution of EPS for a balance between self-protection and EET under environmental pressure. Geobacter prevailed by the addition of QS signals to resist high chlortetracycline concentration. Our results provided a broader understanding on regulating EABs within electrode interface to improve their performance for environmental remediation and clean energy development.


INTRODUCTION
As a broad-spectrum antibiotic, chlortetracycline (CTC) is widely used in animal husbandry due to its strong antibacterial activity against a variety of pathogens. However, the discharge of inadequately treated CTC wastewater into the environment poses a serious threat to human health . Generally, wastewater containing CTC can be treated by the processes based on membrane filtration (Monzon et al., 2016), photocatalysis (Zhang et al., 2017b), adsorption (Zhang et al., 2020), and so on. However, certain defects, such as susceptibility to environmental impacts, high costs, and secondary pollution block their ways for wide application. On the other side, as an environmentally friendly bioelectrochemical technology to convert chemical energy stored in wastewater into clean electricity, microbial fuel cells (MFCs) provide an alternative to treat wastewater/pollutants, which received much attention in environmental remediation in the past decade (Yong et al., 2014).
A large number of studies have reported that the anode biofilms of MFCs can degrade antibiotic contained in wastewater containing antibiotics, such as sulfamonomethoxine (Jiang et al., 2020), salinomycin (Cheng et al., 2020), and sulfamethoxazole (Wang et al., 2016), since it has the advantages of lower cost, better treatment efficiency, as well as the simultaneous generation of clean energy. When treating wastewater containing pollutants, exoelectrogens require higher tolerance and electron transfer ability to maintain their metabolism. As previously reported, mixed bacteria culture has a stronger capability than pure bacteria in resisting the stimulation of external environmental pressure and toxic pollutants. Specifically, our previous reports demonstrated that fermentative bacteria degrade complex organics into small molecules that can be further utilized by exoelectrogens in the anode chambers of MFCs (Li et al., 2021). This synergetic effect is the basis for simultaneous degradation of organic pollutions and generation of clean power by mixed electroactive biofilms (EABs) in MFCs (Li et al., , 2021. Similar to general biofilms, the formation of EABs is mainly divided into four stages: 1) Attachment of microbial cells on electrode; 2) Secretion of extracellular polymeric substrates (EPS) and biofilm formation; 3) Cells growth and biofilm maturation; 4) Cells detachment and biofilm regeneration (Czerwinska-Glowka Additionally, numerous studies have found that QS signals play an important role in regulating the removal of pollutants in various species of microorganisms and communities (Kang and Park, 2010;Zhang et al., 2015). It is also reported that the degradation of nicotine was almost 100% with the addition of QS signals while that was less than 20% in quorum-quenching (QS was interrupted by some specific measures) reactors (Zhang et al., 2015). As previously reported, QS signals could accelerate the biofilm formation and enhance its resistance to harsh external environment. Nevertheless, to the best of our knowledge, few studies focused on the synergistic effects to active response for a good balance between self-protection and EET under environmental pressure among mixed organisms. Therefore, it is necessary to understand the role of QS signals in the formation of EABs and the relationship between power generation and pollutants degradation within electrode interface.
In this study, two kinds of QS signals (N-butyl homoserine lactones, C4-HSL; quinolone, PQS) were supplied to the anode chambers of MFCs to 1) clarify their influence on the electrochemical performance; 2) illustrate the regulation of QS signals on the structure, composition, and activity of EABs; 3) and establish a QS-regulated bioelectrochemical strategy for efficient degradation of CTC. Results showed that QS signals shortened the maturation period of EABs by promoting their enrichment, increasing EPS secretion, and enhancing the EET by decreasing the charge transfer resistance. Consequently, the shaped EABs promoted the performance of CTC degradation and electricity generation simultaneously. This study provides an alternative strategy to modulate the electrochemical performance of MFCs and broaden its potential for application in environmental remediation.

RESULTS AND DISCUSSION
QS signals accelerated the start-up and increased the power output of MFCs The influence of QS signals on the electrochemical performance of MFCs was first analyzed based on the variation of voltage output during the enrichment stage (without the addition of CTC). The voltage output of the MFCs with the addition of C4-HSL and PQS increased significantly in the third cycle, which stabilized after the fifth cycle and maintained with the maximum values around 0.62 and 0.58 V, respectively (Figure S1). However, the voltage output of the MFC without QS signals increased slowly in the early stage and the maximum voltage of each cycle was significantly lower than that of the C4-HSL and PQS groups. Specifically, the voltage output of the Blank group stabled at 0.51 V in the sixth cycle, which was 17.74% and 12.07% lower than that of the C4-HSL and PQS groups, respectively. In addition, the voltage output of abiotic groups was negligible compared with that of the biotic groups supplemented with C4-HSL and PQS ( Figure S2). Therefore, we concluded that the voltage output was attributed to the metabolism iScience Article of exoelectrogens on EABs and the performance difference of these MFCs was due to the impact of C4-HSL and PQS on microorganism.
Interestingly, QS signals also accelerated the start-up of the MFCs. To be more specific, the start-up time of C4-HSL and PQS groups was both 240.35 h, which was 19.94% shorter than that of the Blank group (300.22 h). It is well known that the exoelectrogens attached on anode surface breaked the electron donors intracellular through anaerobic oxidation and then transferred the generated electrons to electrode to form a voltage output (Guo et al., 2020). The faster started-up and stabilization of the MFCs with C4-HSL and PQS indicated that QS signals reinforced the attachment of exoelectrogens to form EABs on anodes, which promoted EET and increased the maximum output voltage by 21.57% and 13.73%, respectively, compared to that of the Blank group. Meanwhile, the maximum power density (0.35 and 0.33 W/m 2 ) also increased by 34.62% and 26.92% with the addition of C4-HSL and PQS, respectively ( Figure 1).
Furthermore, different concentrations of CTC were added to anode chambers to acclimate the EABs for simultaneously degrading CTC and generating electricity after enrichment. Based on the effect of CTC on the activity of EABs, the inhibition of CTC on the voltage output in four batches was calculated according to the equation (Text. S4). The maximum voltage of the three MFCs groups stabilized after nearly 600 h' enrichment, which possibly indicated that the formation of EABs matured on the anode at this stage (Figure S1). When CTC concentration increased from 5 to 30 mg/L, the voltage outputs were all suppressed to different degrees in each MFCs groups ( Figure 2) attributed to the toxicity of CTC. The maximum voltage outputs corresponding to the C4-HSL, PQS, and Blank groups at 30 mg/L CTC were 0.52, 0.50, and 0.41 V, which were respectively 15.65%, 14.66%, and 17.20% lower than that in enrichment stage ( Table 1). The inhibition rates of voltage output in C4-HSL and PQS groups were apparently lower than that in Blank group, indicating that QS signals exerted a beneficial effect on the tolerance of EABs with the increased acclimation duration. Changes in external environment are a significant reason for the changes of physiological activity of microorganism . Interestingly, the different inhibition rates between the C4-HSL group and PQS group under CTC domestication stage were nonnegligible, which could be due to the different effects of two QS signals on the regulation of metabolic pathways (Mukherjee and Bassler, 2019). At this time, the microbial community needed to adjust their structure and abundance, as well as regulating the gene expression, to response and resist the toxicity of CTC to make their metabolism consistent with the physiological needs for using CTC as energy supplier and electron donor (Ma et al., 2021).

QS signals enhanced the bioelectrochemical degradation of CTC
CTC was degraded in anode chambers of all three MFCs groups (without glucose), indicating that the mixed microorganisms embedded in EABs can use CTC as the sole carbon source for metabolism and power generation, which is consistent with the above results ( Figure 2). At the same time, abiotic control iScience Article experiment (without strains) confirmed that the removal of CTC was not the result of adsorption by carbon felts (data not shown). It is worth noting that the CTC degradation efficiency of the C4-HSL and PQS groups was 85.85% and 82.85%, respectively, which were 1.56 and 1.50 times that of Blank group (55.20%) (Figure 3A). Similar to the closed-circuit treatments, CTC was also removed in the C4-HSL, PQS, and Blank group within 24 h under the open-circuit anaerobic fermentative controls, with the degradation rates of 50.10%, 49.40%, and 39.05%, respectively. Obviously, however, the degradation of CTC could be improved significantly by supplying QS signals no matter that in the electrochemical degradation under closed circuit or anaerobic fermentative degradation under open circuit. More importantly, we found that the lower concentration of CTC was observed in the MFCs than that of the fermentative runs ( Figures 3A and 3B), which also showed the superiority of electrochemical treatment of pollutants . In previous report, CTC was converted into lower toxic intermediates in the biosystem . Although the intermediates of CTC were not detected in this study, we could speculate that CTC was degraded into lower toxic intermediates with low molecular weight, which was favorable for the survival of microorganisms. The same conclusion was reported in our previous study (Li et al., 2019b).
In addition, the rate constants of CTC degradation in C4-HSL and PQS groups were 2.19 and 1.86 times (under electrochemical degradation) and 1.35 and 1.42 times (under fermentative degradation) of the Blank group, respectively (Table S3, Figure S3), which were calculated by the degradation kinetic equation. As discussed above, the rate constants of CTC degradation in MFCs were all superior than that in the fermentative systems. This might be related to the synergetic effect between the mixed bacteria species in the EABs, which was discussed as following.

QS signals regulated the activity of the EABs
It has been reported that the three-dimensional EABs on anode improved the bacterial tolerance to toxic pollutants and thus the performance in removing organic pollutants also been significantly enhanced (Li  Voltammetry is a dynamic technique which is sensitive to both electron transfer kinetics and major mass transfer at the electrochemical interface. As the sweep speed increased, the difference between the oxidation and reduction peak potentials as well as the catalytic currents of the three MFCs groups increased, indicating the presence of redox substances at the interface between the anode surface and the solution formed parts of EET of the EABs. There was no obvious difference between the oxidation peak and reduction peak potential of the three MFCs groups under the same scanning speed ( Figure S4), which indicated that the addition of exogenous QS signals neither induced the EABs to produce new redox substances nor acted as electron transfer shutters. Nevertheless, the catalytic currents of the C4-HSL and PQS groups were 21.35 and 19.98 mA at a sweep rate of 0.05 v/s, respectively, which were 50.04%, and 40.40% higher than those of the Blank group (14.23 mA) ( Figure S4). This demonstrated that the activity of the intrinsic redox substances was significantly increased due to the addition of QS signals .
In addition, the electron migration rate constants of the C4-HSL and PQS groups were calculated to be 0.030 G 0.0068 and 0.027 G 0.0019, respectively, which were 1.58 and 1.42 times than those of the Blank group (0.019 G 0.0021).
The enhanced electron migration rate was a significant feature of the decreased resistance of the EABs. Electrochemical impedance (EIS) was employed to assess the conductivity and the electron diffusion ability of the EABs in the MFCs with QS signals. Generally, the charge transfer resistance (Rct) can be obtained from the diameter of the semicircle in the Nyquist curve represents . As shown in Figure 4, the Rct of the three MFCs groups decreased from 3.41 U (Blank) to 1.93 U (C4-HSL) and 2.26 U (PQS), highlighting the effectiveness of QS signals in enhancing electrons transfer between anode surface and EABs.
On the other hand, the solution resistance (Rs) in all groups had no obvious difference. Considering Rct occurs between electrode and EABs, this observation may be involved in the composition of EPS wrapping around the cells (discussed below).

Morphological characteristics of the EABs
The cell morphology embedded in EABs on the anode surface of the three MFCs groups was detected by SEM ( Figure S5). The amount of bacteria cells attached on the anode surface at the initial stage of biofilm enrichment (first cycle) was relatively low. In the middle of the enrichment period (fourth cycle), however, EABs exhibited obvious three-dimensional and complex structures compared with that of the initial stage, which was in accordance with the increased voltage output. In the fifth cycle (and the sixth cycle of the Blank group), the voltage output of each MFCs group was stabilized ( Figure S1), indicating that the EABs matured

EPS composition and biomass regulated by QS signals
The mixed bacteria attached on anodes surface were wrapped with uneven thickness EPS and gradually formed EABs during enrichment. Cells in EABs have been thought to transfer electrons between redox radical in the EPS and insoluble electrode (Gao et al., 2019). Here, the biomass density increased from 290.32 (Blank) to 390.98 (C4-HSL) and 366.33 mg/mL/cm 2 (PQS) (Figure 5), which was in good according to the results of SEM It could be seen that the two QS signals showed various impact on the evolution of EABs, which could be revealed from the voltage output, CTC degradation efficiency, EIS, and biomass density. The differences could be resulted from the direct effect by C4-HSL and/or indirect effect by PQS in the QS system (Brindhadevi et al., 2020;Schuster et al., 2013).
The spatial distances and compact architecture enabled EPS nonnegligible effects in self-protective property and EET. Electrons generated by exoelectrogens cannot easily cross the dense EPS layer to electrode directly (Yang et al., 2019). Fortunately, several electrochemical active substrates embedded in EPS, such as extracellular redox proteins, were certificated to transport electrons between the outer membrane of exoelectrogens and electrodes to facilitate EET process (Xiao et al., 2017). Therefore, the protein content in EPS has a great impact on EET rate. As shown in Figure 5B, the protein content of EPS in the C4-HSL and PQS groups was 180.56 and 169.13 mg/mL/cm 2 , respectively, which was significantly elevated compared to that of the Blank group (110.68 mg/mL/cm 2 ). The enhancement in polysaccharide content was also obvious, which increased from 90.10 (Blank) to 130.08 (C4-HSL) and 129.59 mg/mL/cm 2 (PQS). The rise in polysaccharides content was closely related to the response of biofilms to the changes of external environment, while proteins contain a variety of redox functional groups that facilitated the transfer of electrons outside the cell (Hou et al., 2020).
Surprisedly, QS signals have less effect on the change of polysaccharide content but more on that of protein content. It was speculated that the effects of QS signals have reached the threshold for microbial attachment and response to external stress after the maturation of biofilm; however, their own physical and chemical properties had different effects on electron transfer, resulting in significant differences in protein content in EPS. The higher efficient EET in the EPS of C4-HSL group was obtained compared with other groups, due to the fact that proteins in EPS contained multiple types of redox molecules, while polysaccharides were widely regarded as the insulative substrates (Li et al., 2021;Xiao et al., 2017). When EABs were under the regulation of QS signals, it was favorable for inducing higher content of protein and polysaccharide in this work, which was beneficial to format a heteropical EPS (Li et al., 2021). Moreover, as previous reports, the maximum EET generated by EABs was positively related to the protein content in EPS iScience Article (Yang et al., 2019). The similar result (the negative relevance between Rct and protein content in the EPS) was obtained in Figure S6 (R 2 = 0.99), which agreed with the previous report that the increase in the content of conductive protein accelerates the rate of EET in the EABs.
Additionally, the viability of the EABs of the three MFCs groups was analyzed with CLSM, which demonstrated that cells activity in C4-HSL and PQS groups maintained well after a period of stable operation ( Figure 6). This was due to the fact that QS signals accelerated the metabolism and communication among species in EABs, which in turn produced a thicker biofilm layer and higher biomass. This played an important role in stabilizing the structure and function of the EABs. As the thickness of the biofilm increasing, the inner cells have a restricted access to nutrients, and their cellular activity also relatively weakened (Sun et al., 2015). In this study, however, EABs under the regulation of QS signals contained fewer inactive cells compared with that of the Blank group, which elaborated that the higher viability of EABs could be achieved with the addition of QS signals. It is well known that the live cells are accountable for electron production (Yang et al., 2019), and the inactive cells might be a reason for the declined viability and blocked EET, thus diminished the EABs' performance.

Dynamic shifts in the microbial community after enrichment and CTC domestication
The microbial diversity of the EABs was analyzed at the end of the biofilm enrichment and CTC domestication to reveal the effects of QS signals on the composition of EABs. At the phylum level ( Figure 7A), the EABs of the three MFCs groups in the biofilm enrichment and domestication stages are mainly composed of Firmicutes, Bacteroidetes, and Proteobacteria. It has been reported that Firmicutes and Proteobacteria are main exoelectrogens, which possessed electron-transfer pathways that electrically connected intracellular oxidative catabolic reactions to extracellular terminal electron acceptors (Chen et al., 2017a). However, during the biofilm enrichment stage, the addition of QS signals increased the proportion of Proteobacteria from 43.93% (Blank) to 62.93% (C4-HSL) and 53.44% (PQS), but decreased the percentage of Firmicutes from 52.36% (Blank) to 29.38% (C4-HSL) and 41.81% (PQS). QS mediated by C4-HSL and PQS was present in Gram-negative bacteria (Waters and Bassler, 2005), which could explain why the percentage of Proteobacteria (Gram-negative bacteria) and Firmicutes (Gram-positive bacteria) showed the opposite trend.  (Zhang et al., 2017a), which could be the reason for the increased proportion after CTC domestication. In addition, CTC significantly decreased the voltage output of the three MFCs groups. We speculated that Firmicutes may act as CTC degradation biocatalyst, while Proteobacteria had a greater role on power output. As shown in this study, QS signals have different effects on EABs, namely, increasing the percentage of the exoelectrogens during the enrichment while increasing the CTC Additionally, at the genus level ( Figure 7B), the microbial composition of the EABs in all MFCs groups was mainly Lactococcus, Geobacter, and Enterococcus. Lactococcus was reported to belong to fermentative strain (Cavanagh et al., 2015), which accounted for 47.31% in the Blank group, and further decreased to 22.4% in the C4-HSL group and 26.7% in the PQS group during the biofilm enrichment stage. The same declining trend of Lactococcus was observed after CTC domestication, in which the relative abundance of Lactococcus decreased from 22.01% (Blank) to 6.33% (C4-HSL) and 7.69% (PQS), respectively. In contrast, the percentage of Geobacter, which was considered as a typical exoelectrogens in MFCs (Yang et al., 2019) increased from 9.66% (Blank) to 28.01% (C4-HSL) and 24.17% (PQS) during enrichment, and from 3.76% (Blank) to 23.51% (C4-HSL) and 19.17% (PQS) after CTC domestication. On the other hand, Enterococcus became the dominant specie in EABs after CTC domestication with the proportion of 47.64% (C4-HSL), 42.96% (PQS), and 38.26% (Blank), respectively. It was reported that Enterococcus was the dominant genus for the degradation of tetracycline antibiotics (Zahid et al., 2017). Consequently, under the stress of relative high concentration of CTC, it was firstly considered to degrade CTC to weaken the negative effect on the EABs, which could be explained that the percentage of Enterococcus increased after CTC addition. Undoubtedly, we found that the proportion of Enterococcus in QS signals groups was higher than that of the Blank group, which could be the result from the positive regulation on EABs by QS signals to resist the stress of high concentration of CTC. Moreover, Lactococcus and Enterococcus belong to Gram-positive bacteria while Geobacter belongs to Gram-negative bacteria. C4-HSL and PQS used in this study were reported to play an important role in the QS systems of Gram-negative bacteria (Brindhadevi et al., 2020;Schuster et al., 2013). Therefore, the proportion of Geobacter increased during EABs maturation in the QS group of inoculated MFCs, which resulted in a relative decline in the proportion of Lactococcus and Enterococcus. Furthermore, the microbial composition of EABs was accordingly altered by the dual regulation of CTC and QS signals to adapt to the change of complex external environment due to the addition of CTC. This dynamic shift was consistent with the variable voltage output and the change in diversity and abundance indices . Although the two QS signals (C4-HSL and PQS) were mainly reported to affect Gram-negative microorganism rather than Gram-positive microorganism, we could not exclude that the microorganism regulated by QS signals attempted to communicate with other functional species (such as CTC degradative bacteria) to optimize a better survival environment. Generally speaking, fermentative bacteria gradually decreased with the addition of QS signals, while that of exoelectrogens and CTC-degrading bacteria increased, which varied the performance of the EABs confirmed by the above analyses. The results also indicated that the evolution of EABs varied greatly under the external environment pressure (CTC tolerance in this study), which may be attributed to the different responses and adaptabilities to the QS signals among different microorganisms species. To be more specific, the proportion of exoelectrogens significantly increased while that of fermentative bacteria decreased due to that the QS signals promoted the proportion of CTC-degrading species to reduce the toxicity of CTC and alleviated the unfavorable effects on the exoelectrogens simultaneously.

Conclusion
In this study, we revealed that QS signals accelerated the evolution of EABs, shortened the start-up time, and increased the voltage output of MFCs systems. The percentage of exoelectrogens was significantly increased under the regulation of QS signals, which reduced the charge transfer resistance of the EABs, and thus enhanced EET within the interface between EABs and anodes. Under the tolerance of CTC, the QS signals further increased the proportion of Enterococcus for the efficient degradation of CTC, which moderated the stress of CTC on the performance of exoelectrogens by the positive synergistic effect among mixed species embedded in EABs. These results indicated that QS signals provided a feasible strategy for improving the performance of bioelectrochemical systems (BESs) in electricity production and the treatment of wastewater for sustainable bioremediation.

Limitations of the study
Here, we concluded that QS signals induced the heterogeneous distribution of EPS to active response for a good balance between self-protection and EET under environmental pressure. The scope of this study is limited to the bench-scale bioelectrochemical systems. We investigated the effects of QS signals (10 mmol/L) on power output, chlortetracycline degradation, and structure of EABs in MFCs treating wastewater containing CTC. Whether this concentration could exhibit the best effect in the mix culture system for pilot-scale bioelectrochemical systems is deserved further study. Besides, the detail understanding of the EPS composition with several electrochemical active substrates in EABs could help us to further understand the effects of QS signals on extracellular electron transfer, which was also limited in this study. In sum, the results provided a broader understanding on the regulation of EABs within electrode interface to improve their performance for environmental remediation and clean energy development.

STAR+METHODS
Detailed methods are provided in the online version of this paper and include the following:

DECLARATION OF INTERESTS
iScience Article Electrochemical testing Cyclic voltammetry (CV) was performed in three-electrode system using an electrochemical workstation (PGSTAT302N, Metrohm) for better understanding about the redox ability of the bioanodes and EET between the anode and EABs. The anode (carbon felt), cathode (carbon felt), and Ag/AgCl electrode were employed as the working electrode, counting electrode, and reference electrode, respectively (Izadi et al., 2020). Different sweep speeds (0.01, 0.02, 0.05, 0.07, 0.08, 0.10 V/s) were selected with a sweep interval of À0.8 to 0.8 V. According to Laviron theory (Laviron and Roullier, 1980), the apparent electron transfer rate was calculated as a linear function of the logarithm of the potential of the redox peaks at different scanning speeds.
The polarization and power density curves of the MFC were measured by varying the external resistance (600-10,000U). The current density (I=U/RA) and power density (P=UI) were calculated from the electrode surface area (4.0 cm 2 ), where U is the cell voltage (V), R is the external resistance value, and A is the electrode surface area (Li et al., 2021).
The electrochemical impedance spectroscopy (EIS) was employed in three-electrode system, which was same to the CV experiment, to obtain the ohmic and charge transfer resistances (Izadi et al., 2020), and the results were fitted using ZSim-demo software.
The inhibition rate of different concentrations of CTC on the voltage output of related MFCs in four batches.

Inhibition ð%Þ
where Vmax is the maximum output voltage for each group of MFCs during enrichment, V is the maximum voltage for different batches at different concentrations of CTC domestication.

Morphological characteristics, activity, and community analysis of EABs
The microscopic morphology of EABs at the early (first cycle), middle (fourth cycle), and late (eighth cycle) stages during enrichment were observed by scanning electron microscopy (SEM). The biomass of EABs was measured by the BCA protein kit (Thermo, USA). Each group of EABs was stained with Live/Dead kit (L7007, Thermo Fisher Scientific Inc, USA), and then the bioactivity and 3D structure of EAB were observed with a laser confocal microscope (CLSM, LEICA DM6000B, Germany).

EPS extraction and measurement
The EPS from the EABs in the late stage of biofilm enrichment was extracted and collected following a method described previously. In brief, the carbon felt in the anode chamber was cut and placed in an EP tube containing 30 mL of pure water, followed by adding 2% EDTA (5 mL) and diluting to 50 mL with pure water. The mixture were shaken and extracted for 5 h at 20 C, and then centrifuged at 14,000 rpm for 20 min. Finally, the supernatant was filtered through a filter membrane to obtain EPS. The Polysaccharide and protein concentrations were measured using the sulfuric acid-phenol method and BCA protein Assay Kit (Thermo, USA) (Li et al., 2021), respectively.
CTC degradation curve and kinetic curve CTC (20 mg/L) was added to the MFC anode chamber after gradient acclimation, and the concentration was detected by high performance liquid chromatography every 2 h. A CTC degradation kinetic curve was established according to the following equation: where C0 is the initial CTC concentration (20 mg/L), Ct is the CTC concentration at time t, and K is the firstorder kinetic rate coefficient, t is sampling time.