Elsevier

Immunobiology

Volume 220, Issue 1, January 2015, Pages 60-67
Immunobiology

Blockage of Wnt/β-catenin signaling by quercetin reduces survival and proliferation of B-1 cells in vitro

https://doi.org/10.1016/j.imbio.2014.09.001Get rights and content
Under a Creative Commons license
open access

Abstract

The Wnt/β-catenin signaling pathway has been shown to play an important role in controlling the proliferation, survival and differentiation of hematopoietic cells. Several Wnt/β-catenin signaling components influence hematopoietic cells fate. B-1 cells are self-renewing and spontaneously express both myeloid and lymphoid restricted transcription factors. B-1 lymphocytes play a major role in autoimmunity and are related to CD5+ B-cell lymphomas and leukemias, such as CLL (chronic lymphocytic leukemia). Herein, we demonstrate that Wnt/β-catenin pathway is important to B-1 cell survival in vitro. The loss of Wnt signals by quercetin treatment induces a reduction in the proliferation and survival of B-1 cells. Furthermore, the quercetin treatment diminishes IL-6 production by peritoneal cells, a cytokine important to the maintenance of B-1 cells in vitro. Importantly, the IL-6 addition to B-1 cell culture prevents cells from apoptosis, even in the presence of quercetin. These data suggest that a deregulation in β-catenin signals could result in alterations in B-1 cell proliferation and differentiation. The correlation between Wnt/β-catenin and IL-6 could point out a mechanism for the expansion of B-1 cells in autoimmune disease and neoplasia.

Abbreviations

APC
adenomatous polyposis coli
CLL
chronic lymphocytic leukemia
Fz
frizzled receptor
GSK-3β
glycogen synthase kinase
HSC
hematopoietic stem cells
LDL
low-density lipoprotein
Lef
lymphoid enhancing factor
LRP
LDL receptor-related protein
PCR
polymerase chain reaction
TLR
toll like receptor

Keywords

β-Catenin
B-1 cells
IL-6
Proliferation
Quercetin
Wnt

Cited by (0)