Targeting expression to projection neurons that innervate specific mushroom body calyx and antennal lobe glomeruli in larval Drosophila
Section snippets
Screening for projection neuron GAL4 lines
Specific GAL4 lines are an essential tool to study the development, connectivity and function of the nervous system. In the olfactory system of Drosophila the development of PNs that connect the antennal lobe (AL) and higher olfactory centers such as mushroom bodies (MBs) and lateral horn has been studied using the GH146 projection neuron GAL4 line. This line labels about 90 PNs in anterodorsal and lateral clusters of PNs, representing two-thirds of the projection neurons in adults (Jefferis et
Database screening and flies
An image database (Awasaki and Ito, unpublished data) of the NP collection of GAL4 lines (Hayashi et al., 2002) was screened for lines that label the larval AL. Larval brain expression patterns were visualized by crossing GAL4 lines to P{UAS-mCD8::GFP}LL6 (Lee and Luo, 1999) or nSyb::GFP (Ito et al., 1998). Brains were dissected and labeled with antibodies to GFP and Discs-large, as previously described (Masuda-Nakagawa et al., 2005). Secondary antibodies were anti-rabbit Alexa 488 and
Acknowledgements
We acknowledge the Bloomington Drosophila Stock Center for fly stocks, and to the Developmental Studies Hybridoma Bank, University of Iowa, for anti-Discs-large antibody. This work was supported by a grant from the Japan Society for the Promotion of Science to L.M.M.-N.
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Present address: Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA.