Research briefHigh annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
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Acknowledgments
This work was support by the TRF/BIOTEC Special Program for Biodiversity Research and Training Grant BRT 542084. Special thanks are extended to the Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University and the Applied Technology in Biodiversity Research Unit, Institute for Science and Technology Research and Development, Chiang Mai University, Thailand.
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2022, Veterinary Parasitology: Regional Studies and ReportsCitation Excerpt :Hence, DNA based identification can't be prior used for species confirmation in current study but it can be used to differentiate from previous Orthocoelium in database. Only three species of Orthocoelium, namely O. streptocoelium, O. parvipapillatum, and O. dicranocoelium, have previously been recorded in Thailand (Eduardo, 1985; Sey et al., 1997: Sripalwit et al., 2007; Anuracpreeda et al., 2016). From phylogenetic analyses, Orthocoelium specimens from the current study could not be identified as O. streptocoelium because of high nucleotide substitution or genetic distance differences.
Fischoederius elongatus (Poirier, 1883) Stiles & Goldberger, 1910, a cryptic species of pouched amphistome (Gastrothylacidae)?
2021, Molecular and Biochemical ParasitologyCitation Excerpt :Worms that were transferred into phosphate buffered saline developed a long, slender appearance and large specimens reached a body length >2 cm. Based on the description of F. elongatus in the literature [3] and previous reports from Thailand [11,12] we originally assumed that the collected flukes represented this species. Unexpectedly, a first analysis of the COX1 mtDNA from three specimens revealed considerable sequence difference of one specimen to the other two specimens (data not shown).
A review of molecular identification tools for the opisthorchioidea
2021, Journal of Microbiological MethodsRumen fluke, Fischoederius elongatus (Trematoda: Gastrothylacidae): Preliminary investigation of suitable conditions for egg hatching
2020, Veterinary ParasitologyCitation Excerpt :Therefore, various reliable classical and modern techniques can be combined to further validate trematode identification and classification to species level (Ichikawa et al., 2013; Laidemitt et al., 2017; Jadav et al., 2018; Shameem et al., 2018; Mohanta et al., 2019). F. elongatus is an amphistome species commonly found in Thailand (Sey, 1991; Sey and Prasitirat, 1994; Sripalwit et al., 2007). Due to its life cycle that is similar to general amphistomes, several periods of their transmission stages depend on host and environment factors which are related to their survival and distribution.
Development and validation of a mtDNA multiplex PCR for identification and discrimination of Calicophoron daubneyi and Fasciola hepatica in the Galba truncatula snail
2013, Veterinary ParasitologyCitation Excerpt :Later studies have confirmed the usefulness of Polymerase Chain Reaction (PCR) techniques for detecting F. hepatica infection in G. truncatula (Caron et al., 2008, 2011; Kozak and Wedrychowicz, 2010), as well as in other Lymnaea species (Magalhães et al., 2004; Cucher et al., 2006). Molecular techniques have hardly been used for the study of Paramphistomidae (Sripalwit et al., 2007; Bazsalovicsová et al., 2010; Sanabria et al., 2011; Lotfy et al., 2010), and specifically in the case of C. daubneyi, only one technique has been described for the identification of adult worms, based on the use of the second internal transcribed spacer (ITS-2) of ribosomal DNA (Rinaldi et al., 2005). Bearing all the above in mind, the aim of this study was to develop and validate a sensitive and specific analytical method based on a mitochondrial (mtDNA) multiplex PCR technique for one-step early detection of infections by C. daubneyi and F. hepatica in the intermediate host snail.