Research brief
High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand

https://doi.org/10.1016/j.exppara.2006.05.005Get rights and content

Abstract

The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers.

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Acknowledgments

This work was support by the TRF/BIOTEC Special Program for Biodiversity Research and Training Grant BRT 542084. Special thanks are extended to the Parasitology Research Laboratory, Department of Biology, Faculty of Science, Chiang Mai University and the Applied Technology in Biodiversity Research Unit, Institute for Science and Technology Research and Development, Chiang Mai University, Thailand.

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