Effect of gemini surfactant (16-6-16) on the synthesis of silver nanoparticles: A facile approach for antibacterial application
Introduction
The emerging advances in nanotechnology led to the designing of engineered noble metal nanoparticles (NMNPs), which offer realand radically new opportunities in the field of biomedicine and material science [1], [2], [3], [4]. Silver nanoparticles (AgNPs) are one of The most widely studied nanomaterials, exhibiting potential applications in high sensitivity bimolecular detection, catalysts, surface-enhanced Raman scattering (SERS), metal electrodes, microelectronics, and diagnostics and Plasmonic Photo Thermal Therapy (PPTT), catalytic activity, purification of water, surfaces and air [5], [6], [7], [8], [9], [10] also possessing broads pectrum antimicrobial activities toward Gram positive and Gram negative bacteria including viruses [11], [12], [13], [14], [15].
Indeed, several synthetic strategies have been formulated in order to successively fabricate the silver nanoparticles (AgNPs) using chemical reduction process [7], [16], [17], [18], [19], [20], [21], [22]. Other methods include thermal decomposition, micro-emulsion, pulsed laser ablation, chemical reduction method is highly, flexible simple and cost-effective to obtain AgNPs by altering the pair of reducing- capping agents. The controlling of capping agents (i.e., steric polymers, charged polymers, anionic, cationic and non-ionic surfactants, saccharine, proteins and gelatin) play a crucial role in the tuning the morphology of the synthesized AgNPs [7], [16], [17], [18], [20], [21]. Hence, the choosing of capping agent has great concern to design the monodisperse and biocompatible AgNPs.
The anionic surfactant, SDS were used in larger number of research works to tune the shapes [20]. In Our previous work on non-ionic surfactant (TX-100) [23] and cationic surfactant CTAC (cetyltrimethylammonium chloride) [24] stabilized AgNPs explains the formation kinetics of monodispersed spherical AgNPs through critical micelle concentration (CMC) and cloud point (CP) investigations [25].
The fundamental application problem of AgNPs is connected with the sufficient stability of their dispersions allowing the prevention of the aggregation process because the generation of spacious aggregates leads to a loss of the antibacterial activity [26], [27]. Therefore, various surfactants and polymers are commonly applied to stabilize these metal colloids. Although the stability is considered to be a crucial property of the AgNPs dispersions, there is still lack of studies employing its tests. There are many reports are available where conventional surfactant used as capping agent. There are a few reports on the Gemini surfactants role on the synthesis of nanoparticles (NPs). Therefore, it is very important to understand role of the Gemini surfactants on the synthesis of NPs. In the present work, we report the role of Gemini surfactant, 1, 6-Bis (N, N-hexadecyldimethylammonium) adipate, 16-6-16 (using as capping agent) on the synthesis of AgNPs.
The dimeric or gemini surfactants are made up of two amphiphilic moieties which connected at the level of the head groups or very close to the head groups by a spacer group [28], [29], [30], [31], [32], [33], [34]. Gemini surfactants have two hydrophilic groups and two hydrophobic groups per molecule, rather than the single hydrophilic and single hydrophobic group of conventional surfactants. The greater efficiency and effectiveness of geminis (viz., surface activity) 10 to 100 times more efficient, lower CMC (CMC is at least one order of magnitude lower, solubilization (better solubilizing), low Kraft temperature, etc.) over comparable conventional surfactants [34] make them more cost-effective as well as environmentally desirable.
However, there are a few report usages of Gemini surfactants to synthesis the nanostructured materials. Henceforth, it is worth to investigate the impact this capping agent towards controlling the nucleation and growth of AgNPs. In this present study is aimed at the evaluation of the stabilization effects of Gemini surfactants on nearly monodisperse AgNPs. As a result, we observed an excellent stability in the Gemini surfactants capped AgNPs. The synthesized materials were characterized by UV–vis spectroscopy, DLS, Zeta potential (ZP), XRD, HRTEM and EDX techniques in order to investigate their optical, morphological and elemental composition. Finally, antibacterial efficiency of the synthesized AgNPs was carried out against Gram-negative and Gram-positive bacterium, viz. Escherichia coli and Staphylococcus aureus, respectively
Section snippets
Materials
Silver nitrate (≥99.9%), sodium borohydride (≥99%) and other fine chemicals were purchased from Sigma, India. The Gemini surfactant, 1, 6-Bis (N, N-hexadecyldimethylammonium) adipate, (16-6-16) see Fig. 1 was synthesized. Milli-Q water (1–2 μS cm−1 at 25 °C) was used as a solvent throughout the experiments.
Bacterial culture
Escherichia coli (MTCC 062) and Staphylococcus aureus (MTCC-3160) were purchased from the Institute of Microbial Technology, India, and grown in nutrient agar (0.3% beef extract, 0.5% peptone
Synthesis and characterization of AgNPs
Silver nanoparticles were synthesized according to the method described in the experimental section, the colloidal solution turned golden brown after adding NaBH4 indicating formation of the AgNPs. UV–vis spectroscopy is one of the most widely used techniques for structural characterization of AgNPs. The optical absorption spectrum of metal NPs is dominated by the SPR which exhibits a shift towards the red end or blue end depending upon the particle size, shape, state of aggregation and the
Conclusions
We report the effect of capping agent Gemini surfactant (16-6-16) during the synthesis of AgNPs. The synthesized AgNPs were characterized by UV–vis spectroscopy, XRD, HRTEM, and EDX in order to investigate the morphologies and chemical compositions of AgNPs. UV–vis spectra show the characteristic localized surface plasmon absorption peak (LSPR) of the synthesized AgNPs (λmax) at 400 nm. In this report, size of the gemini surfactant (16-6-16) capped AgNPs is varied from 2 nm to 17 nm showing
Acknowledgements
Authors are thankful to the financial support from project STRAIT (CSC 0201) of the Council of Scientific and Industrial Research (CSIR), New Delhi, India (CSIR-CLRI Communication No. 1223). One of the authors T. Parandhaman acknowledges UGC for Rajiv Gandhi National Fellowship.
References (43)
- et al.
Facile synthesis of silver nanoparticles with high concentration via a CTAB-induced silver mirror reaction
Colloids Surf. A
(2012) - et al.
Superior bactericidal activity of SDS capped silver nanoparticles: synthesis and characterization
Mater. Sci. Eng. C
(2009) Dimeric and oligomeric surfactants. Behavior at interfaces and in aqueous solution: a review
Adv. Colloid Interface Sci.
(2002)- et al.
Antimicrobial behavior of biosynthesized silica–silver nanocomposite for water disinfection: a mechanistic perspective
J. Hazard. Mater.
(2015) - et al.
Synthesis and characterization of gallium colloidal nanoparticles
J. Colloid Interface Sci.
(2010) - et al.
Organically modified silica nanoparticles: a nonviral vector for in vivo gene delivery and expression in the brain
Proc. Natl. Acad. Sci. U. S. A.
(2005) - et al.
Immobilized silver nanoparticles enhance contact killing and show highest efficacy: elucidation of the mechanism of bactericidal action of silver
Nanoscale
(2013) - et al.
Antibacterial activity of glutathione-coated silver nanoparticles against gram positive and gram negative bacteria
Langmuir
(2012) - et al.
SERS effects in silver‐decorated cylindrical nanopores
Small
(2011) - et al.
Anisotropic nanomaterials: structure, growth, assembly, and functions
Nano Rev.
(2011)
Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives
Adv. Nat. Sci.: Nanosci. Nanotechnol.
Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy
RSC Adv.
The synthesis of size‐and color‐controlled silver nanoparticles by using microwave heating and their enhanced catalytic activity by localized surface plasmon resonance
Angew. Chem. Int. Ed.
Polyacrylate-assisted size control of silver nanoparticles and their catalytic activity
Chem. Mater.
Analysis of silver nanoparticles in antimicrobial products using surface-enhanced Raman spectroscopy (SERS)
Environ. Sci. Technol.
Hyperbranched poly (amidoamine) as the stabilizer and reductant to prepare colloid silver nanoparticles in situ and their antibacterial activity
J. Phys. Chem. C
Interfacing engineered nanoparticles with biological systems: anticipating adverse nano–bio interactions
Small
Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs)
J. Phys. Chem. C
Metal Nanoclusters in Catalysis and Materials Science: The Issue of Size Control: The Issue of Size Control
Prevention of photooxidation of deoxymyoglobin and reduced cytochrome c during enhanced raman measurements: SERRS with thiol-protected Ag nanoparticles and a TERS technique
J. Phys. Chem. C
Synthesis of highly monodisperse citrate-stabilized silver nanoparticles of up to 200 nm: kinetic control and catalytic properties
Chem. Mater.
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