Draft genome sequences data of Mammaliicoccus lentus isolated from horse farm soil

Mammallicoccus lentus is a member of the commensal microflora of the Staphylococcaceae family, which colonizes the skin of several species of farm animals, including poultry and dairy animals (Huber et al., 2011; Zhang et al., 2009). The study of the members of the Staphylococcaceae family, such as the Mammaliicoccus genus, isolated from various sources is of great importance for agriculture and public health as contributes to the accumulation of knowledge and understanding of the mechanisms of antibiotic resistance gene transmission among bacterial pathogens. This thesis is supported by recent studies showing that some members of the Mammallicoccus genus serve as a reservoir of virulence and antibiotic resistance genes and may also be a source of horizontal gene transfer (Saraiva et al., 2021). Here, we present a draft genome sequence of Mammallicoccus lentus strain PVZ.22 from a horse farm soil sample. The sequencing was performed on the Illumina MiSeq platform. The genome was assembled using the Geneious software package. The genome contains 2,802,282 bp with a total of 2805 genes, 8 perfect and 12 strict AMR genes and 58 tRNAs genes.


a b s t r a c t
Mammallicoccus lentus is a member of the commensal microflora of the Staphylococcaceae family, which colonizes the skin of several species of farm animals, including poultry and dairy animals (Huber et al., 2011;Zhang et al., 2009).The study of the members of the Staphylococcaceae family, such as the Mammaliicoccus genus, isolated from various sources is of great importance for agriculture and public health as contributes to the accumulation of knowledge and understanding of the mechanisms of antibiotic resistance gene transmission among bacterial pathogens.This thesis is supported by recent studies showing that some members of the Mammallicoccus genus serve as a reservoir of virulence and antibiotic resistance genes and may also be a source of horizontal gene transfer (Saraiva et al., 2021).Here, we present a draft genome sequence of Mammallicoccus lentus strain PVZ.22 from a horse farm soil sample.The sequencing was performed on the Illumina MiSeq platform.The genome was assembled using the Geneious software package.

Value of the Data
• The complete genome sequence of Mammaliicoccus lentus isolated from horse farm soil provides information that may be helpful for understanding the epidemiology of bacterial infections in domestic animals.• The analyses of antibiotic resistance genes can be used to predict the probability of the strain being a multidrug resistance pathogen.• The data may assist researchers in study of antimicrobial resistance genes.
• The data can be used in study of bacterial pathogenicity evolution.
• The data can be used by researchers for genomics and other evolutionary studies.
Taxonomic affiliation of the strain was predicted by whole genome comparison using the genome-to-genome distance calculator (GGDC) and OrthoANI values.The most closely related microorganism found in the GenBank database was Mammaliicoccus lentus strain H29 ( Table 2 ).
The genome was screened for antimicrobial resistance genes against the Comprehensive Antibiotic Resistance Database (CARD; card.mcmaster.ca).The predicted resistance genes in the genome are presented in Table 3 .

Experimental Design, Materials and Methods
Mammaliicoccus lentus strain PVZ.22 was isolated from horse farm soil in Kazakhstan (43.184873N 76.523994 E).The pure isolate was cultured in commercially prepared tryptic soy agar (Conda, Spain) with 5 % sheep red blood cells.The initial species identification was carried out by visually (non-hemolytic, white opaque colonies) and light microscopy (Gram positive cocci clustered in grape-like aggregates) [6 , 7] .
For DNA extraction, culture was grown in nutrient broth (Conda, Spain) for 24 h at 37 °C.The DNA was extracted using PureLink Genomic DNA Mini Kit (Invitrogen, USA) according to the manufacturer's protocol.The quality of the resulting DNA was determined using the Tecan's NanoQuant Plate (Tecan, Männedorf, Switzerland) at the optical wavelengths of 260 and 280 nm.The DNA library was prepared using the Nextera XT DNA sample preparation kit (Illumina, Cambridge, UK), and quantified using the Qubit 3.0 and Qubit dsDNA High Sensitivity Kit (Invitrogen, USA).The library was then sequenced on the Illumina MiSeq platform with 2 × 300 bp reads.The raw Illumina reads were trimmed and assembled de novo with with SPAdes 3.12.0(an installable plugin in Geneious Prime) [8][9][10] .A complete genome of Mammaliicoccus lentus strain H29 chromosome (CP059679) was used as reference genome.The annotation was added by the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) [11] .Information about PGAP can be found here: https://www.ncbi.nlm.nih.gov/genome/annotation_ prok/ .The Resistance Gene Identifier (RGI v5.2.0) was used for screening of antimicrobial resistance genes [12] .The OrthoANIu tool [13] was used to compare prokaryotic genome sequence when classifying and identifying bacteria ( https://www.ezbiocloud.net/tools/ani(assessed 17.08.2023).The identification of CRISPR arrays and their associated Cas proteins was performed using the CRISPRCasFinder 4.2.20 software [14] .
The raw reads are available online at the Mendeley repository [15] .The complete chromosomal sequence was deposited under the accession number -NZ_CP116807.

Ethics Statements
Work did not include animal experiments or data collected from social media platforms or human subjects.

Data Availability
Raw sequence data (Original data) .
The genome contains 2,802,282 bp with a total of 2805 genes, 8 perfect and 12 strict AMR genes and 58 tRNAs genes.© 2023 The Author(s).Published by Elsevier Inc.This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )