Whole genome sequencing data of Chromobacterium amazonense BASUSDA_45 isolated from soil in Bangladesh capable of degrading pesticide

This article reports the Chromobacterium amazonense BASUSDA_45 strain's draft genomic sequence. The bacterium was isolated from cypermethrin pesticide contaminated soil and then the sequencing was carried out. Initially de novo assembly of the raw sequences, trimming and quality check generates 125 contigs having N50 of 78,923. Further mapping of the contigs generated scaffolds. The genome contains 53 scaffolds with a total length of 4,295,151 bp having 62.30% GC content and N50 of 3,726,017. Annotation using Prokaryotic Genome Annotation Pipeline (PGAP) reveals 4181 genes among which 4096 were coding sequences, 76 tRNAs, 3 rRNAs, 4 noncoding RNAs. The raw sequence reads and annotated genome were uploaded to NCBI's Bioproject repository with the accession number PRJNA686506.


a b s t r a c t
This article reports the Chromobacterium amazonense BA-SUSDA_45 strain's draft genomic sequence. The bacterium was isolated from cypermethrin pesticide contaminated soil and then the sequencing was carried out. Initially de novo assembly of the raw sequences, trimming and quality check generates 125 contigs having N50 of 78,923. Further mapping of the contigs generated scaffolds. The genome contains 53 scaffolds with a total length of 4,295,151 bp having 62.30% GC content and N50 of 3,726,017. Annotation using Prokaryotic Genome Annotation Pipeline (PGAP) reveals 4181 genes among which 4096 were coding sequences, 76 tRNAs, 3 rRNAs, 4 noncoding RNAs. The raw sequence reads and annotated genome were uploaded to NCBI's Bioproject repository with the accession number PRJNA686506.

Value of the Data
• The strain is capable in degrading pesticide (cypermethrin) supplemented into the media as the sole carbon source for their growth, development and metabolism. The mechanism is yet to be revealed how this bacterium use and utilize pesticide for their survival. Following utilization, this bacterium greatly degrades pesticide, reducing toxic waste and can be an eco-friendly measure to help protecting the environment. • The reported data for the C. amazonense strain BASUSDA_45 is very significant for the both fundamental and applied microbial research as a means of eco-friendly environmental control. • The sequencing data can be further analyzed to find particular gene that degrades pesticides and other genes that utilize pesticide residues as energy source. Again, particular pathways that involve these processes can give detail insights. • Researchers that are involved in and finding ways to reduce toxic chemicals from environment can be highly benefitted from these data.

Data Description
Chromobacterium amazonense is a Gram negative bacterium mostly found in soil and water [1] . They are free-living bacteria able to survive under different adverse environmental conditions [2] . C. amazonense strain BASUSDA_45 was isolated from pesticide contaminated soil. We reported the whole genome sequencing data of Chromobacterium amazonense strain BA-SUSDA_45, here.

Source of Inoculum and Bacterial Culture
The sample was collected from pesticide contaminated soil of Netrokona, Bangladesh (24.87947N 90.70843 E) where pesticides had been continuously used for at least 10years. The soil was collected at a depth of 25 cm from the surface and serial dilution was carried out until adequate colonies are obtained. From that, pure culture was done and DNA extraction was performed.

DNA Extraction
The extraction of DNA was carried out by TIANamp bacteria DNA kit (Tiangen Biotech Co. Ltd., Beijing, China) following the manufacturer's protocol. From these onward downstream processes were conducted by sequencing service provider Invent Technologies Ltd. (Dhaka, Bangladesh).

Ethics Statements
The permission was obtained from Institute of Biological Sciences, University of Rajshahi for the sampling of the soil microbes. This was approved in the "Resolution No. of the 71th meeting of the Board of Governors of the Institute of Biological Sciences and Resolution No. 57 of the meeting of the Syndicate of the University of Rajshahi, Bangladesh."

Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.