RNA-seq data of Aspergillus tubingensis NBRC 31125 in carbon catabolite repressor related to xylanase production

Aspergillus tubingensis NBRC 31125 is a prolific producer of endo-xylanase and β-xylosidase. However, the presence of glucose in the medium causes carbon catabolite repression (CCR) which inhibits the secretion of those enzymes. CCR in Aspergillus has been investigated in several ways. However, there are currently not any molecular data are available regarding the CCR of A. tubingensis in the xylan and glucose medium. Therefore, this research focuses on this aspect. The RNA of the strain was extracted in repressive condition, followed by sequencing using the Illumina NextSeq550 platform and reference assembly. The RNA-seq raw reads were submitted to the NCBI website's Sequence Read Archive database under the accession numbers SRR15412365 and SRR15412366, respectively. The data provide information for differentiating the response of xylanase and other enzymes production with and without glucose addition. The transcriptomics data can also be used to understand the xylan metabolism and CCR in Aspergillus

glucose in the medium causes carbon catabolite repression (CCR) which inhibits the secretion of those enzymes. CCR in Aspergillus has been investigated in several ways. However, there are currently not any molecular data are available regarding the CCR of A. tubingensis in the xylan and glucose medium. Therefore, this research focuses on this aspect. The RNA of the strain was extracted in repressive condition, followed by sequencing using the Illumina NextSeq550 platform and reference assembly. The RNA-seq raw reads were submitted to the NCBI website's Sequence Read Archive database under the accession numbers SRR15412365 and SRR15412366, respectively. The data provide information for differentiating the response of xylanase and other enzymes production with and without glucose addition. The transcriptomics data can also be used to understand the xylan metabolism and CCR in Aspergillus

Value of the Data
• The transcriptomics data on the effect of glucose addition in A. tubingensis NBRC 31125 grown in the xylan medium contribute to understanding the mechanism of carbon catabolite repression (CCR) related to xylanase at the molecular level. • The transcriptomics data would be useful to researchers focusing on enzyme production using fungi and working on transcriptional regulation. • The data obtained can be further analysed as the differentially expressed gene and transcript expression patterns of A. tubingensis NBRC 31125. This could lead to identification of genes related to CCR and future improvement of new strains of A. tubingensis through molecular engineering to increase the xylanase production • The data can also be utilised for comparison with transcriptome analyses of other Aspergillus species or fungi under repressive conditions in order to identify predominant CCR patterns in fungi.

Data Description
The raw RNA sequencing data of A. tubingensis NBRC 31125, inoculated in the xylan medium containing glucose and xylan medium alone was stored in the FASTQ file and collected to the NCBI-SRA database with accession numbers SRR15412365 and SRR15412366, respectively. The descriptive RNA-seq data statistics of both samples are given in Table 1 . Clean reads were mapped against the genome reference of Aspergillus tubingensis WU-2223L. Fig. 1 shows the difference in gene expression of A. tubingensis NBRC 31125 grown in xylan medium alone and xylan medium containing glucose in heatmap visualisation. The graph was constructed from the abundance estimates of raw transcripts of the fungi grown in such mediums. A dendrogram and  colour bar were presented on the left side of the heatmap indicating the gene's clustering. The detail of the genes can be seen in Table S1 (supplementary data). The darker colour showed the higher gene transcription, conversely the lighter colour showed less transcription.

Cultivation
Aspergillus tubingensis NBRC 31125 was grown in the Potato Dextrose Agar at a temperature of 30 °C for seven days. The fungi were inoculated in a liquid medium using techniques suggested by Chand  .0, and 10g/L of xylan from a corn cob (Sisco) . A total of 50 g/L glucose was added to the xylan medium to induce a repressive condition. Erlenmeyer flasks were incubated at a temperature of 30 °C for five days [1][2][3] . After incubation, the mycelium was collected for RNA extraction. All experiments were performed in duplicate.

Extraction and Sequencing of RNA
Total RNA was extracted from the mycelium of both samples (duplicate) using Direct-Zol RNA Miniprep Plus (Zymo Research) according to the manufacturer's instructions. RNA concentration and quality were determined by Nanodrop Spectrophotometer and Agilent 2100 Bioanalyser. The performed works were rRNA removing, RNA fragmentation, double-stranded cDNA synthesis, adenylated-end addition, adapter addition, PCR amplification, library-quality test, and sequencing on next-generation sequencing NextSeq550 platforms.

Data workflow of RNA Sequencing
The clean reads were obtained by removing the raw reads of RNA-seq with adaptors. The quality clean reads were analysed using the software FastQC v.011.9 and MultiQC v1.1 [4 , 5] . The reads were stored in the FASTQ file. The data was mapped into Aspergillus tubingensis WU-2223L genome (GCF_013340325.1) as a genome reference [6] . Kallisto v.0461 was used to quantify the abundance estimates of raw transcripts [7] . Data visualisation was performed using T-REx v2.0 [8] .

Ethics Statements
Not applicable.

Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Data Availability
Raw reads of A. tubingensis NBRC 31125 grown in xylan and xylan containing glucose medium (Original data) (DIB).

Supplementary Materials
Supplementary material associated with this article can be found, in the online version, at doi: 10.1016/j.dib.2022.108700 .