Metabarcoding dataset on the elicitation of Soybean and Mungbean using Ragi Tape as elicitors for enhancing secondary metabolites production

Ragi Tape (RT) is commonly used as the microbial starter for various Indonesian traditional food. Consist of diverse microbial populations, RT has excellent agents as elicitors to augment the bioactive compounds in Soybean (SB) and Mungbean (MB). Metabarcoding analysis has shown to identify the microbial population involved in elicitation using RT. Inoculated RT from SB and MB were collected to extract the microbial DNA for the post-elicitation group. In comparison, DNA extraction from powdered RT was conducted as a pre-elicitation group. The total DNA were then sequenced by using the MiSeq Illumina platform with 16S rDNA gene region V3-V4 and 18S rDNA gene region V4 as a biomarker for bacterial and fungal identification, correspondingly. The obtained raw-data sequences were then analyzed using QIIME2 pipeline. According to the number of the acquired sequences, the 18S sequencing yielded more DNA strands than the 16S sequencing. However, the number of assigned OTUs was higher in 16S sequences than 18S sequences. From the perspective of the sample, RT has larger distinctive taxa, which were not identified in other samples. This metagenome data will provide fundamental information on RT employment in the elicitation process and further understanding of elicitation mechanisms using RT as biotic elicitors. The data is available at the BioProject database under the NCBI domain with accession no. PRJNA767401.


a b s t r a c t
Ragi Tape (RT) is commonly used as the microbial starter for various Indonesian traditional food. Consist of diverse microbial populations, RT has excellent agents as elicitors to augment the bioactive compounds in Soybean (SB) and Mungbean (MB). Metabarcoding analysis has shown to identify the microbial population involved in elicitation using RT. Inoculated RT from SB and MB were collected to extract the microbial DNA for the post-elicitation group. In comparison, DNA extraction from powdered RT was conducted as a preelicitation group. The total DNA were then sequenced by using the MiSeq Illumina platform with 16S rDNA gene region V3-V4 and 18S rDNA gene region V4 as a biomarker for bacterial and fungal identification, correspondingly. The obtained raw-data sequences were then analyzed using QIIME2 pipeline. According to the number of the acquired sequences, the 18S sequencing yielded more DNA strands than the 16S sequencing. However, the number of assigned OTUs was higher in 16S sequences than 18S sequences. From the perspective of the sample, RT has larger distinctive taxa, which were not identified in other samples. This metagenome data will provide fundamental information on RT employment in the elicitation process and further understanding of elicitation mechanisms using RT as biotic elicitors.

Value of the Data
• The complete information on bacterial and fungal taxa in Ragi Tape will provide valuable information on the development and quality control of Ragi Tape production, along with future development for extensive application purposes. • The data will interest researchers studying conventional biotechnology employing Ragi Tape and elicitation to improve the plant's secondary metabolites production. • The microbial diversity data from these sequence reads may be used to further understand the roles of bacteria and fungi in modulating secondary metabolites production in plant, particularly in host-elicitors interaction during elicitation. • Several key taxa such as Pantoea sp. Enterobacter sp., Bacillus sp., Lactobacillus fermentum , and Saccharomycopsis fibuligera provide a promising candidates for future development of secondary metabolites in larger-scale production.

Data Description
The data in this article describe the diversity of bacterial and fungal taxa from RT as elicitors for SB and MB [1] . All of the raw data reads have been deposited on NCBI SRA with the accession number as shown in Table 1 . The obtained 16S sequences ranged from 54,519 to 72,571. On the other hand, up to 67,152; 70,104; and 64,938 reads were obtained from the 18S rDNA Table 1 Detail information of every dataset in NCBI SRA database.

No.
Sample Name Barcode Treatment Accession no.
Ragi Tape -elicited mungbean 18S Elicitation SRX14239565 Fig. 1. The total number of obtained sequences from sequencing and denoising-filtering step (A) and the number of assigned OTUs from each sample (B). Correspondingly, the frequency of assigned and unassigned sequences in 16S (C) and 18S sequences (D). In addition, the number of distinctive and shared OTUs from 16S (E) and 18S sequences (F) is also described.
sequencing from RT, SB, and MB, respectively. Almost half of the 16S raw sequences did not pass the quality control. For 18S sequences, the sequences categorized as candidate taxa (or feature) were 44,126; 49,036; and 46,483 for RT, SB, and MB. However, only 37%-55.63% of 16S sequences and 65.71%-71.58% of 18S sequences were successfully assigned into particular taxa ( Fig. 1 A). The 16S sequences were assigned greater than 18S sequences, meaning that bacterial populations were more diverse than fungal populations ( Fig. 1 B). The data also suggest that bacteria have a larger frequency than fungi ( Fig. 1 C and D). Nevertheless, non-fungal OTUs were also identified from 18S sequences ( Fig. 1 D). Based on the assigned OTUs in each biomarker, 11 OTUs from 16S sequences were shared in all samples ( Fig. 1 E). While only 5 OTUs from 18S sequences were co-founded in RT, SB, and MB ( Fig. 1 F). Interestingly, RT has a higher number of distinctive taxa than other samples, both from 16S or 18S taxonomic assignments ( Fig. 1 E and  F). The detail on the assigned OTUs as described in the table S1 and S2.

Samples
Ragi Tape (RT) was purchased from a local market in Sawojajar, Malang, East Java, Indonesia. Soybean (SB) var. Anjasmoro and Mungbean (MB) var. Vima-1 were obtained from the Research Center of Legumes and Tuber Plants (BALITKABI), Malang, East Java, Indonesia.

DNA extraction
Elicitation was performed prior to DNA isolation step using the previously described method [1] . Upon elicitation, the beans (SB and MB) were rinsed with sterile distilled water to elute the microbial communities from the beans' surface. The eluted water was used as DNA isolation samples. The DNA isolation from RT was done by diluting the powdered RT into sterile distilled water (0.1 g/mL) and mixed using vortex for 10 minutes. All samples (SB, MB, and RT) were then passed to the DNA isolation process with DNeasy PowerFood Microbial Kit (Qiagen, Germany) according to manufacturer protocols. The quality of DNA was evaluated using NanoDrop Spectrophotometer and Qubit Fluorometer prior to sequencing step.

Ethics Statements
This study does not include an animal or human involvement as subjects.

Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.

Data Availability
An Insight of Bacterial and Fungal Population in the Elicitation of Soybean and Mungbean using Ragi Tape (Indonesian Traditional Food Fermenter) (Original data) (NCBI Sequence Read Archive (BioProject)).