In vitro treatment of human granulosa cells with irisin and leptin: Quantitative RT-PCR array data (female infertility panel)

Reproduction is closely related to energy metabolism: physical activity and adiposity (either insufficient weight or obesity) can affect female fertility. Irisin is a myo- and adipokine produced by skeletal muscles during exercise or shivering as well as in smaller amounts by subcutaneous visceral adipocytes [1]. Leptin is a neuroendocrine adipokine regulating satiety and energy expenditure. Circulating levels of both, irisin and leptin, correlate with adiposity status and physical activity [2], [3], [4], [5], [6]. This article presents data from quantitative PCR array of the in vitro effects of irisin and leptin on cultured human ovarian granulosa cells. Granulosa cells were purified from follicular fluid samples obtained from women undergoing in vitro fertilization (IVF) procedure and were subjected to treatment with irisin (500 ng/mL) or leptin (100 ng/mL) for 24 h. The array included 84 genes involved in female fertility.


a b s t r a c t
Reproduction is closely related to energy metabolism: physical activity and adiposity (either insufficient weight or obesity) can affect female fertility. Irisin is a myo-and adipokine produced by skeletal muscles during exercise or shivering as well as in smaller amounts by subcutaneous visceral adipocytes [1] . Leptin is a neuroendocrine adipokine regulating satiety and energy expenditure. Circulating levels of both, irisin and leptin, correlate with adiposity status and physical activity [2][3][4][5][6] . This article presents data from quantitative PCR array of the in vitro effects of irisin and leptin on cultured human ovarian granulosa cells. Granulosa cells were purified from follicular fluid samples obtained from women undergoing in vitro fertilization (IVF) procedure and were subjected to treatment with irisin (500 ng/mL) or leptin (100 ng/mL) for 24 h. The array included 84 genes involved in female fertility.  Table   Subject Health and medical sciences Specific subject area Female reproductive endocrinology Type of data Table  Figure  How the data were acquired Quantitative RT-PCR array using RT 2 Profiler PCR Array -Human Female Infertility kit (Qiagen) and QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). Data were analyzed using GeneGlobe Data Analysis Center (Qiagen). Data format Raw and Analyzed Description of data collection Human ovarian granulosa cells were purified from follicular fluid samples obtained during IVF procedure. Cells were cultured in vitro and were subjected to treatment with irisin (500 ng/mL) or leptin (100 ng/mL) for 24 h. The effect of irisin and leptin on modulation of 84 genes involved in female infertility was evaluated by using qRT-PCR array. Data source location

Value of the Data
• Provided data are useful for understanding the relationship between energy metabolism and reproduction. • Presented data may be of interest to researchers and physicians working in the field of reproduction. • This dataset can be used as a basis for further investigations of the molecular mechanisms of irisin and leptin action in the ovary.

Data Description
This article presents data obtained from qRT-PCR array measuring the expression of various genes related to female infertility. Human ovarian granulosa cells purified from follicular fluid samples were grown in vitro and treated with irisin or leptin for 24 h. Array included 84 genes listed in Tables 1 and 2 . Raw data from the analysis (C t values) have been published in a data repository [7] . Analyzed data demonstrating fold-regulation and fold-change of irisin or leptin treatment vs. control are presented in Tables 3 and 4

Data calculation and statistical analysis
Obtained threshold cycle (Ct) values from the PCR array were analyzed using GeneGlobe Data Analysis Center ( https://geneglobe.qiagen.com/us/analyze ). Gene expression values were normalized using arithmetic mean of the Ct values and the following housekeeping genes: ACTB, B2M, GAPDH, HPRT1, POLP0. Data were accepted as statistically significant if p ≤ 0.05.

Ethics Statements
This research was approved by the Institutional Review Board of Northwell Health (IRB # 20-0449). Informed consent was obtained from all individuals involved in the study.

Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.