Transcriptome dataset of ethylene-treated Klutuk Wulung banana

Klutuk Wulung banana (Musa balbisiana Colla, BB Group) is a climacteric fruit whose ripening is influenced by ethylene production. This banana fruit has a relatively slow ripening process time and long shelf-life compared with A genome banana (Musa acuminata, AA). Bananas are usually harvested at a pre-climacteric stage and ripened artificially by exogenous ethylene. Hence, the application of exogenous ethylene at the pre-climacteric stage can accelerate the Klutuk Wulung banana ripening. However, there is no report regarding the effect of exogenous ethylene treatment on Klutuk Wulung banana global gene expression. The knowledge of global gene expression of ethylene treated Klutuk Wulung banana will help to understand this fruit ripening process. In this study, global gene expression data of untreated and ethylene treated Klutuk Wulung banana fruit during ripening were available. Total RNA was extracted from fruit pulp for differential expressed gene analysis using RNA-Seq. The RNA-Seq results obtained were ranged from 34,565,252 to 44,752,129 total reads, with 80.5% to 86.7% of reads were mapped against Klutuk Wulung banana genome reference derived from The Banana Genome Hub. In total, 29,968,128 to 37,776,907 transcripts were detected. The transcriptome data discussed in this article were deposited into NCBI's Gene Expression Omnibus (GEO) Series with an accession number GSE162077. These data can be used as information to identify gene candidates involved in fruit ripening for the application in banana postharvest program.


a b s t r a c t
Klutuk Wulung banana ( Musa balbisiana Colla, BB Group) is a climacteric fruit whose ripening is influenced by ethylene production. This banana fruit has a relatively slow ripening process time and long shelf-life compared with A genome banana ( Musa acuminata , AA). Bananas are usually harvested at a pre-climacteric stage and ripened artificially by exogenous ethylene. Hence, the application of exogenous ethylene at the pre-climacteric stage can accelerate the Klutuk Wulung banana ripening. However, there is no report regarding the effect of exogenous ethylene treatment on Klutuk Wulung banana global gene expression. The knowledge of global gene expression of ethylene treated Klutuk Wulung banana will help to understand this fruit ripening process. In this study, global gene expression data of untreated and ethylene treated Klutuk Wulung banana fruit during ripening were available. Total RNA was extracted from fruit pulp for differential expressed gene analysis using RNA-Seq. The RNA-Seq results obtained were ranged from 34,565,252 to 44,752,129 total reads, with 80.5% to 86.7% of reads were mapped against Klutuk Wulung banana genome reference derived from The Banana Genome Hub. In total, 29,968,128 to 37,776,907 transcripts were detected. The transcriptome data discussed in this article were deposited into NCBI's Gene Expression Omnibus (GEO) Series with an accession number GSE162077. These data can be used as information to identify gene candidates involved in fruit ripening for the application in banana postharvest program.

Value of the Data
• Musa balbisiana Colla is a B genome banana that has a slower ripening process and longer shelf-life than A genome banana. This data provides a potential genetic source for postharvest management. • This data provides a comprehensive transcriptomic analysis using pair-end sequencing with two to three biological replicate datasets to understand the metabolic pathways affected by ripening and ethylene treatment. • This data will help to explain the mechanisms of Klutuk Wulung banana fruit ripening and identify the genes that are expressed differently on ethylene treatment.

Data Description
Klutuk Wulung Banana ( Musa balbisiana Colla, BB Group) is a climacteric fruit whose ripening is influenced by ethylene. The fruit has a relatively slower ripening process and longer shelf-life than A genome banana ( Musa acuminata , AA Group). According to Maduwanthi and Marapana [1] , bananas are usually harvested at the pre-climacteric stage and ripened artificially by exposure to exogenous ethylene. Plantain bananas (B-content genome) can only be consumed 18 days after harvest without being treated with ethylene at a storage temperature of 25-27 °C, while A genome banana can be consumed 6 days after harvest [2 -5] . Delays of finger-drop or release of fingers and hands from bunches due to maturity in B-content banana were also found in a study conducted by Imsabai et al. [6] . As a result, B genome bananas became the target of postharvest technology development programs with the characteristics of high-stress resistance and long ripening time-related traits [7] . Table 1 The transcriptome's raw data output statistics of Musa balbisiana (BB Group) 'Klutuk Wulung' fruit. This data was generated from day 1 control ( K1 ), day 1 ethylene-treatment ( E1 ), day 7 control ( K7 ), and day 7 ethylene-treatment ( E7 ) of Musa balbisiana (BB Group) 'Klutuk Wulung' fruit in each replicate (replicate A, B , and C ) of the paired-end experiment: forward reads ( 1 ) and reverse reads ( 2 Hence, the application of exogenous ethylene treatment can accelerate Klutuk Wulung banana fruit ripening and the biological process can be revealed by using transcriptomic analysis. In the previous study, the transcriptomic approach was successfully provided the transcriptome data and revealed global gene expression of chitosan-coated and uncoated banana fruit during ripening, hence provided data for identifying candidate genes involved in the delay of fruit ripening by chitosan coating [8] . Therefore, the transcriptomics data of ethylene-treated Klutuk Wulung banana is important to provide basic information for further candidate genes analysis involved in fruit ripening in response to ethylene treatment of B genome banana to design a better postharvest technology and management on bananas. The data on this article were included the transcriptomics of ethylene-treated (100 μl/L) and untreated (control) of Klutuk Wulung banana (B genome banana). The global gene expression changes from the transcriptomics during ripening of ethylene-treated and untreated Klutuk Wulung bananas were evaluated. The files of transcriptomics dataset, which were generated from 17 libraries of raw data and 10 sets of processed data, has been submitted to Gene Expression Omnibus (GEO) NCBI database [9] .
Control and ethylene-treated Klutuk Wulung banana ( M. balbisiana Colla, BB Group) RNA library were successfully sequenced and the raw data were deposited in NCBI's Sequence Read Archive (SRA) database with an accession number SRP293766 ( https://www.ncbi.nlm.nih.gov/ sra?term=SRP293766 ). The raw data statistics could be seen in Table 1 . According to Table 1 , total nucleotide, total reads, and GC percentage in this study are sufficient for further analysis since the values of total nucleotides and total reads are constant in each replicate. Moreover, good quality of RNA-Seq data using Illumina sequencing has a criterion of about 40-50% GC content [6] . This GC percentage is almost similar in transcriptomic data analysis of 12 different tissues of M. acuminata and M. balbisiana , which ranged from 40 to 48% [10] . Moreover, RNA-seq analysis conducted by Dwivany et al. [8] found that the raw data statistics of control and chitosan-coated M. acuminata subgroup Cavendish fruit pulp has GC content of 48-51%. This suggests that these data could be used for further analysis, which continued to the transcriptome assembly and data analysis using Tophat2 [12 , 13] and Cufflinks [13 , 14] to get the processed data.
The statistics summary of processed data is shown as dataset statistics of the mapping result and transcripts detection ( Table 2 ). These data were available at NCBI's Gene Expression  [11] also found that 75.55% M. balbisiana transcripts of 12 different tissues were mapped against the whole genome reference which could be used for further analysis. Moreover, Dwivany et al. [8] also found that the transcripts of Cavendish banana ( M. acuminata ) fruit pulp were 75.8-83.8% mapped against the genome reference. Hopefully, this data could be used as a basic information to further analysis of gene candidates involved in fruit ripening using RNA-Seq data, especially on B genome bananas.

Plant materials
Klutuk Wulung banana ( M. balbisiana Colla, BB Group) was harvested from Dago, Bandung, West Java, Indonesia (6 °53'28.9"S, 107 °36'38.3" E). The criteria for Klutuk Wulung banana used in this study were the relative similarity of fruit skin color, physiological age, and fruit size, and also the absence of fungal infection or physical defects as described by Lustriane et al. [5] regarding the sorting of bananas for research purposes. The fruit then was randomly divided into two groups, i.e. a control group (without ethylene treatment; encoded with K ) and an ethylene treatment group (encoded with E ). For the ethylene treatment group, 100 μl/L exogenous ethylene gas was exposed to bananas and carried out in a glass container [15] . Then, the bananas were incubated for 24 h and stored outside the container at room temperature (26 °C ± 1 °C) for 7 days.

Total RNA isolation, library preparation, and sequencing
Total RNA of Klutuk Wulung banana was extracted from banana pulp on the first day (duplicates) and seventh day (triplicates) of fruit ripening using Cordeiro et al.'s method [16] . The RNA concentration was measured using NanoDrop spectrophotometer (Eppendorf BioSpetometer ® Kinetic) at the wavelength of 230, 260, and 280, then performed rRNA bands check using electrophoresis technique on 1.5% agarose gel. RNA was then purified from DNA contaminant using DNAseI kit from Thermo Scientific (Catalog Number: EN0521). The RNA library from Klutuk Wulung was constructed using TrueSeq RNA Sample Prep KIT v2 and was sequenced using Illumina platform HiSeq 2500.
Quality control of each sample was managed to examine

Declaration of Competing Interest
No conflict of interests declared.