Multiple breath washout test data for healthy controls

This article includes pulmonary function data collected via multiple breath nitrogen washout for 103 healthy U.S. adults recruited at National Jewish Health in Denver, Colorado. Testing was performed by certified technicians and reviewed by expert pulmonologists for quality and consistency. Data were collected from a diverse population that included 52 males and 51 females with an average age of 39 years (range 20–77 years). Participants were of non-Hispanic White (85%), African-American/Black (6%), Hispanic (4%), more than one race (4%) or American Indian/Alaskan Native (1%) race/ethnicity. The majority were never smokers (85%), but 12% were former smokers and 3% were current smokers. Height, weight, and body mass index (BMI) were collected in addition to multiple breath washout (MBW) test parameters such as the lung clearance index (LCI) score.


a b s t r a c t
This article includes pulmonary function data collected via multiple breath nitrogen washout for 103 healthy U.S. adults recruited at National Jewish Health in Denver, Colorado. Testing was performed by certified technicians and reviewed by expert pulmonologists for quality and consistency. Data were collected from a diverse population that included 52 males and 51 females with an average age of 39 years (range 20-77 years). Participants were of non-Hispanic White (85%), African-American/Black (6%), Hispanic (4%), more than one race (4%) or American Indian/Alaskan Native (1%) race/ethnicity. The majority were never smokers (85%), but 12% were former smokers and 3% were current smokers. Height, weight, and body mass index (BMI) were collected in addition to multiple breath washout (MBW) test parameters such as the lung clearance index (LCI) score. Table   Subject Pulmonary and Respiratory Medicine Specific subject area Pulmonary physiology measurements using multiple breath nitrogen washout testing Type of data

Value of the Data
• Published normative data for healthy adults using multiple breath washout is limited and has not included a heterogeneous population. • Investigators working with adult lung disease patients will benefit from access to this data as a reference for healthy multiple breath washout values. • Others can build upon this data to create an even more robust set of normative data to be used consistently across study sites.
Raw Data: Raw multiple breath washout data and relevant demographic characteristics are included on the attached Excel file for download. Data from all trials are not provided, rather results are the average of all acceptable trials. A description of the variables in the raw dataset is presented in Table A1 .

Experimental Design, Materials and Methods
Participants were recruited at National Jewish Health in Denver, Colorado between March 2015 and March 2020 as part of a larger study aimed at understanding deployment-related lung diseases. Participants were screened for both previously diagnosed chronic respiratory disease and for acute respiratory illness in the four weeks preceding scheduled testing. All testing was completed in a clinical research testing room with medical air and oxygen hook ups. To confirm healthy control status, pre-bronchodilator spirometry was performed 15 min before multiple breath washout testing using American Thoracic Society Guidelines [ 4 , 5 ]. Participants with forced vital capacity percent predicted (FVCPP), forced expiratory volume in one second percent predicted (FEV1PP), or FEV1/FVC ratio below the lower limits of normal (LLN) based on published reference values were excluded from MBW testing. [6] 25 of the subjects were from the pilot study and did not have spirometry, which was added to data collection after the pilot.
MBW testing was performed using the Eco Medics AG Exhalyzer D system and Spiroware software. On each day of testing, environmental settings including temperature and pressure were adjusted, flow and gas channel calibrations were performed, and signal synchronizations During testing, subjects were seated upright, wore a nose clip, and were advised to perform relaxed breathing. Subjects began normal breathing on room air to establish tidal volume. The washout phase used 100% oxygen and concluded when subjects' nitrogen concentrations were below 1/40 or 2.5% of their initial concentration. Results presented are the average of at least two acceptable trials. Trials were deemed unacceptable if 1) the tracer gas did not re-equilibrate between trials, 2) there was clear evidence of a leak, 3) the breathing pattern was erratic, or 4) the trial did not meet end of test criteria described by Jensen et al. [7] .

Ethics Statement
Study participants agreed to complete testing with written informed consent under studies HS-2851 and HS-2985 approved by the National Jewish Health Institutional Review Board.

Declaration of Competing Interest
Cecile Rose receives research grant funding from the U.S. Department of Defense as part of a large multi-site, multi-investigator study on mechanisms of lung epithelial injury. Silpa Krefft is employed by the U.S. Department of Veterans Affairs (DVA) and receives research grant funding from the DVA. Both Drs. Rose and Krefft have participated in medicolegal depositions to provide expert testimony on patients for whom they have rendered medical opinions; however, they have received no personal income or compensation for these medicolegal efforts, all of which have been reimbursed to National Jewish Health. through the Sergeant Sullivan Fund at National Jewish Health . Supported by NIH / NCATS Colorado CTSA Grant Number UL1 TR002535 . Contents are the authors' sole responsibility and do not necessarily represent official NIH views.

Supplementary Materials
Supplementary material associated with this article can be found in the online version at doi: 10.1016/j.dib.2020.10 6 641 .