Data on the bipolar electroactive conducting polymers for wireless cell stimulation

Data in this article is associated with our research article “Bipolar Electroactive Conducting Polymers for Wireless Cell Stimulation” [1]. Primarily, the present article shows the data of PPy-pTS, PPy-DS and PPy-DS/collagen in conventional electrochemical process and bipolar electrochemical process for comprehensive supplement and comparison to help with better understanding and developing conducting polymers based bipolar electrochemistry. Secondly, the presented data of bipolar electrostimulation (BPES) protocol development constitute the complete dataset useful for modeling the bipolar electroactive conducting polymers focusing on wireless cell stimulation, which are reported in the main article. All data reported were analysed using Origin 2018b 64Bit.


Value of the Data
• The presented data constitute the complete dataset useful for modeling the electroactive doped conducting polymers focusing on the bipolar electrochemistry in wireless cell stimulation point of view. • The data could be used by researchers as starting point when studying or developing similar conducting polymers based bipolar electrochemistry. • The data can support similar analysis, considering both conventional three-electrode system and bipolar electrochemical system in biological environment (PBS buffer) first, further insights could consider about applications in wireless cell stimulation as we proved. • All findings have been adopted to support conducting polymers based bipolar electrochemistry involved in studies related to wireless cell stimulation, which advances the field of biomedical stimulation and controlled release system.

Data Description
The data presented in this article are related to the research article, "Bipolar Electroactive Conducting Polymers for Wireless Cell Stimulation" [1] . The cyclic voltammograms data of synthesizing the PPy-p TS, PPy-DS and PPy-DS/collagen materials are presented in Fig. 1 . The dopants on polypyrrole (PPy) matrix vary from typically small size dopant, p -toluenesulfonic acid  monohydrate ( p TS) to bioinert high molecular weight dopant, dextran sulfate sodium salt from Leuconostoc spp. (DS) and collagen (Type I from rat tail). Comparative CVs and electrochemical impedance spectroscopy (EIS) of these three doped PPy films in PBS are shown in Fig. 2 [2] . Fig. 3 shows the schematic diagram of the devices for bipolar electrochemistry and bipolar electrostimulation (BPES). All spectro-electrochemical data (in situ UV-vis with conventional electrochemical system in Fig. 4 , in situ Raman spectrometry with bipolar electrochemical system in Fig. 5 , ex situ Raman spectrometry and FTIR spectrometry data before and after underwent bipolar electrochemical process in Fig. 6 ) were obtained and analysed to identify the bipolar electrochemical activation was related to the typical electrochemical redox process within PPy films induced by electric field switching [3] . Fig. 7 displays the images of PPy-DS and PPy-DS/collagen after fluorescence labeling using a ZEISS Axiovert microscope. The BPES protocol for the specificdesigned device was developed by optimizing the applied DC voltage under pulse mode. Images of culture media in Fig. 8 , pH indicator papers in Fig. 9 , cell viability in Fig. 10 and schematic illustrations of the waveform of applied pulse mode and three distinct programmed BPES pulse modes used for studies of cell proliferation and cell differentiation in Fig. 11 are reported. The corresponding data in Fig. 12 and Table 1 are comprehensive supplement for data analysis of BPES on cell differentiation. One compressed file that contains all raw data of the graphs being shown (Electrodeposition data in Fig. 1 , CV and EIS data in Fig. 2 , In situ UV data in Fig. 4 , Ex situ FTIR and Raman data in Fig. 6 ) can be available in Mendeley Data, DOI: 10.17632/4zgkyfxhcd.1 .

Fig. 3. Schematic diagram of the cells for (a) bipolar electrochemistry and devices for (b) bipolar electrostimulation (BPES).
In (a), viton ring and the four screws were used to fix the frame closely onto the acrylic bottom. For more convenient observation and application in following in situ spectro-electrochemical experiments, clear acrylic bottom replaced the opaque one. In (b), all parts were carefully positioned and fixed on the glass bottom using silicon adhesive sealant. The SSM strips extending beyond the frame were designed to connect with the external DC power supply. The cell culture wells (1.0 cm 2 /well) were in the middle of the device, which mimicked the one used in conventional electrochemical cell stimulation system. Data are represented as mean ± standard deviation (SD).

Materials synthesis
The preparation of polypyrrole (PPy) films were carried out by cyclic voltammetry (CV) using an CHI-720 Electrochemical Analyzer system in a standard three-electrode electrochemical cell, which configures a platinum (Pt) sheet counter electrode (1 cm × 3 cm), an Ag/AgCl reference electrode, and a FTO-glass working electrode (1 cm × 2 cm). Depositions were obtained from an aqueous solution containing 0.2 M distilled pyrrole (Py) with 0.1 M p TS, 2 mg/ml DS without or with 2 μg/ml collagen within a potential range of 0-0.65 V at a scan rate of 20 mV/s. Different dopants were employed to obtain various PPy -p TS, PPy-DS and PPy-DS/collagen films in order to investigate the effects of dopants on bipolar electrochemical activities. After polymerization, all films were thoroughly rinsed with Milli-Q water and allowed to dry under ambient conditions before further characterization and investigation.

Design and methods
CVs of synthesized PPy-p TS, PPy-DS and PPy-DS/collagen were carried out with a potential range of −0.7 V to + 0.7 V at a scan rate of 100 mV/s, while EIS were performed over the frequency range of 0.1 Hz to 100 kHz using an AC signal with + 50.0 mV vs the reference electrode, using the CHI-720 Electrochemical Analyzer system. In situ UV-vis spectra (Shimadzu UV-vis 3600) were recorded simultaneously with the conventional three-electrodes electrochemical system within the range of 30 0-110 0 nm under different applied potentials (from −0.6 V to + 0.6 V) in PBS (pH = 7.4). In situ and Ex situ Raman spectra (HR800 Raman spectrometer, Japan) were obtained by 10 s data collection within the wavenumber range of 50 0 cm −1 -20 0 0 cm −1 , using excitation laser at 632.81 nm with a low laser power (less than 10 mW) and × 50 WLD objective lens. FTIR spectra were collected using a FT-IR spectrometer (IRpretige-21, Shimadzu) over a range of 600 cm −1 -2000 cm −1 . The surface morphology of fluorescently labelled PPy-DS and PPy-DS/collagen samples were examined in using a ZEISS Axiovert microscope. A micro-Raman spectrometer with 632.81 nm diode laser excitation was utilized. The objective len ( × 50 WLD) was positioned directly above the optical window and focused on the spot of bipolar electrode. The laser spot was around 1 -2 micrometer in diameter with a lower laser power (less than 10 mW) to avoid possible laser irradiation damage of samples. All spectra were acquired by 10 s data collection within the wavenumber range of 50 0-20 0 0 cm −1 . All samples were placed in the middle of designed bipolar cell, immersed into the PBS. For reference, the pristine spectrum was taken from the original film in PBS without the applied driving voltage, and the recovered spectrum showed the Raman spectrum from the film after bipolar testing in PBS with removed driving voltage 1 min later. All the in situ spectra were obtained with different driving voltage for 30 s. B(-) and B( + ) present the Raman spectra from the poles of bipolar electrode, which were opposite the cathodic and anodic driving electrodes. According to the principle of bipolar electrochemistry, the oxidation at B(-) site and reduction at B( + ) site occurred at the same time.  were firstly soaked in 1.5 ml solution with isometric PBS and ethanol for 30 min. Then 500 μl rhodamine red TM -X succunimidyl ester/DMSO at a final concentration of 2.5 μg/ml was added into each well, followed by 5 h' reaction at RT in covered Al foil as darkroom. Finally, replaced with the fresh PBS after thorough washing with isometric PBS and ethanol for 10 min each time, total three times to remove the residue fluorescent dye. The surface morphology of fluorescently labelled samples were examined in using a ZEISS Axiovert microscope.