Draft-genome sequence data and phylogenomic comparison of two marine-sourced bacterial strains Pseudoalteromonas sp. MIP2626 and Psychrobacter sp. BI730

Halophilic and psychrophilic marine bacteria are source of interesting bioactive molecules for biotechnology. We report here the whole-genome sequences of two of them, Pseudoalteromonas sp. MIP2626 isolated from tropical peeled shrimps and Psychrobacter sp. BI730 isolated from deep-sea hydrothermal vent. Sequencing of both genomes was performed by Illumina HiSeq platform (2 × 150 pb). De novo assemblies using Spades v3.9 generated 136 contigs for Pseudoalteromonas MIP2626 and 42 contigs for Psychrobacter BI730, representing a genome size of 3.9 Mb and 3.2 Mb, respectively. Phylogenetic based on 16S rRNA gene sequence and phylogenomic analyses were reported to compare the new sequences with Pseudoalteromonas and Psychrobacter representative strains available in the public databases. The genome sequences have been deposited at GenBank under the accession numbers JAATTW000000000 for Pseudoalteromonas sp. MIP2626 and JAATTV000000000 for Psychrobacter sp. BI730.


a b s t r a c t
Halophilic and psychrophilic marine bacteria are source of interesting bioactive molecules for biotechnology. We report here the whole-genome sequences of two of them, Pseudoalteromonas sp . MIP2626 isolated from tropical peeled shrimps and Psychrobacter sp. BI730 isolated from deep-sea hydrothermal vent. Sequencing of both genomes was performed by Illumina HiSeq platform (2 × 150 pb). De novo assemblies using Spades v3.9 generated 136 contigs for Pseudoalteromonas MIP2626 and 42 contigs for Psychrobacter BI730, representing a genome size of 3.9 Mb and 3.2 Mb, respectively. Phylogenetic based on 16S rRNA gene sequence and phylogenomic analyses were reported to compare the new sequences with Pseudoalteromonas and Psychrobacter representative strains available in the public databases. The genome sequences have been deposited at GenBank under the accession numbers JAATTW0 0 0 0 0 0 0 0 0 for Pseudoalteromonas sp.

Value of the data
• The data contribute to describe the genomic diversity of the Pseudoalteromonas and Psychrobacter species; • Genome sequence data of halophilic strains from marine origin are useful for comparative genomic analysis and highlight the genetic adaptation of these species in food ecosystems; • Genome sequences provide new knowledge on Pseudoalteromonas and Psychrobacter species of biotechnological importance.

Data Description
Pseudoalteromonas and Psychrobacter species are Gram-negative moderate halophilic bacteria that could be isolated in different natural ecosystems like soil, salt lake and marine environment [6] , but also in salted food such as seafood, processed meat and cheese [7][8][9] . As they are psychrophilic bacteria, both genera are interesting for biotechnological applications [10][11][12][13] . In Based on the homology of 16S rRNA gene sequences with those of closely type strains (Additional File 1), Psychrobacter sp . BI730 shares high similarity with Psychrobacter nivimaris 88/2-7 T (99.93% for a 1461 bp sequence) ( Fig. 1 ). In the same way, Pseudoalteromonas sp . MIP2626 strain appears closely related to Pseudoalteromonas nigrifaciens KMM 661 T (100% for a 1458 bp sequence) and Pseudoalteromonas haloplanktis ATCC 14393 T (99.18% for a 1467 bp sequence) ( Fig. 2 ).
Whole Phylogenomic comparison based on type strains or reference genomes available in public databases was performed. Further information about the selected reference genomes are shown in Additional File 2. Psychrobacter sp . BI730 belongs to a group with several marine strains ( Fig. 3 ). No ANI value over 95 % of BI730 strain with the reference genomes available was observed. Pseudoalteromonas sp . MIP2626 is closely related to both food and marine strains ( Fig. 4 ). This strain showed an ANI value higher than 95 % with the type strains Pseudoalteromonas nigrifaciens NCTC10691 T (97.84 %) and Pseudoalteromonas translucida KMM 520 T (96.1 %).

Genomic DNA extraction
BI730 and MIP2626 strains were grown in Zobell medium at 30 °C under aeration and total genomic DNA was extracted using phenol-chloroform protocol [1] .

Phylogenetic analysis of 16S rRNA
The 16S rRNA gene was amplified using 27-F (5'-AGAGTTTGATCATGGCTCA-3') and 1492-R (5'-TACGGTTACCTTGTTACGACTT-3') primers. Thermal cycling conditions were applied as follow (i) 1 min at 94 °C for initial denaturation, (ii) 30 cycles of 1 min at 94 °C for denaturation, 0.5 min at 56 °C for primer annealing, 1.5 min at 72 °C for elongation, and (iii) 5 min at 72 °C to ensure final elongation. DNA amplicons were separated on 0.8% agarose gel, purified by using the ExoSAP-IT (Thermo Fisher Scientific, Waltham, Massachusetts, USA) and sent for sequencing to the service provider (Eurofins Genomics, Ebersberg, Germany). Sequences were analyzed to obtain a preliminary taxonomic classification for each isolate.
We elaborated an initial phylogeny of the isolates BI730 and MIP2626 using the closest related 16S rRNA gene sequences generated by EzBiocloud blast ( https://www.ezbiocloud.net/ ). The GenBank accession numbers of these species are shown in Additional File 1. Further phyloge-

Genome sequencing and assembly
Whole genome sequencing was performed through the Illumina HiSeq platform at GATC-Biotech (Konstanz, Germany) The genomes were assembled with de novo assembly using SPAdes version 3.9 [2] . Then, the contigs were filtered to keep those of length > 300bp and coverage > 100. Annotations were produced using the Rapid Annotations using Subsystems Technology server [3] .

Phylogenomic analysis
Representative complete genome sequences of Psychrobacter and Pseudoalteromonas were collected from the NCBI database. Information about these genomes such as isolation origin, Bioproject and BioSample numbers, and Average Nucleotide Identity (ANI) with BI730 and MIP2626 are shown in Additional File 2. ANI values, were determined by JSpeciesWS [14] . Psychrobacter and Pseudoalteromonas genome sequences selected from the public database were aligned with the BI730 and MIP2626 strains, respectively, using the software Progressive Mauve (version 2.4.0) with the default settings [5] . The phylogenomic trees were visualized with Interactive Tree of Life (iTOL v5) ( http://itol.embl.de ).

Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships which have, or could be perceived to have, influenced the work reported in this article.