Metagenomic dataset on lichen Dirinaria sp. from the Great Rann of Kutch and tropical moist deciduous Dang forest of Gujarat.

This paper describes the additional data to our research article “Bacterial line of defense in Dirinaria lichen from two different ecosystems: First genomic insights of its mycobiont Diriniria sp. GBRC AP01” by Puvar et al. [1]. In this manuscript we are presenting the data obtained during the annotation of the genome enriched from metagenomic data from the lichen samples.


a b s t r a c t
This paper describes the additional data to our research article "Bacterial line of defense in Dirinaria lichen from two different ecosystems: First genomic insights of its mycobiont Diriniria sp. GBRC AP01" by Puvar et al. [1] . In this manuscript we are presenting the data obtained during the annotation of the genome enriched from metagenomic data from the lichen samples.

Value of the data
• The lichen is complex ecosystem having numerous species with majorly fungi and algae or cyanobacteria as symbionts. The Whole shotgun sequence data of lichen from two different eco geographic region revels their diversity, ecosystem and functional attributes in the ecosystem. • The information supports to the claims by Puvar et al. [1] for KEGG pathway, COG and SEED classifications of the lichen metagenomes. • Metagenomic data of Dirinaria lichen reveals the association of bacteria and mycobiont symbiosis along with associated diversity in response to their natural habitat. • Functional attributes observed in the lichen metagenomic data will give insight for further elucidation of microbiome role in local biogeochemical cycling. • The skimmed mycobiont data from lichen metagenome enhances the fungal reference genome database for Dirinaria genus.

Data description
Metagenomic assemblies from raw data generated in [1] are described in Table 1 . which depicts the assembly statistics of the lichen metagenomes. Draft genome was reconstructed using combined approach and basic assembly statistics are described in Table 2 . which summarizes the skimmed mycobiont Diriniria sp. GBRC AP01. Both metagenomic data were analyzed for taxonomic distribution and their top 20 taxa represented as bar chart in Fig. 1 . Similarly, both metagenome assemblies were mapped against KEGG, COG and SEED databases and their relative percent abundance is described in Figs. 2-4 respectively.

Sampling
Dirinaria sp. from two different geographic regions from Gujarat were collected along with their substratum. (a) DG18 was collected from the Dangs district located in southern part of Gujarat covered with semi to dense moist forest. (b) KK01 was collected from Kutch district located western part of the state situated in white salt desert and thorny forest. Both samples were collected during post monsoon seasons along with their substratum i.e. rock.

DNA extraction
Metagenomic DNA was isolated using combined protocol optimized using Qiagen Powersoil kit and Qiagen Plant Mini kit. DNA from each sample (lichen whole thallus) was extracted multiple time to achieve sufficient quantity to perform whole shotgun sequencing i.e. 1 μg DNA in 100 μl volume.

Metagenomic sequencing
About 1 μg of total metagenomic DNA from both samples was used to prepare libraries of 200 bp fragment length using Ion Xpress TM Plus Fragment Library kit and were sequenced using Ion Proton system using Ion PI v2 chip kit [1] .

Data analysis
Metagenome assembly was performed using Megahit [3] with the default parameters except minimum contig length as 200 base pairs and the assembly statistics are described in the Table 1 .

Funding Information
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.