Physical, mechanical, chemical and biological properties data of gellan gum incorporating titanium dioxide nanoparticles biofilm.

Gellan gum incorporating titanium dioxide nanoparticles biofilm was synthesized and characterized using UV, FTIR and XRD to study their physical and chemical properties. The mechanical properties were measured using universal mechanical testing. Meanwhile, the biological properties were investigated towards for antibacterial and cell proliferation. This comprehensive data are relevant with the research article entitled “Gellan gum incorporating titanium dioxide nanoparticles biofilm as wound dressing: Physicochemical, mechanical, antibacterial properties and wound healing studies” [1].


Specifications table
Chemistry, material science Specific subject area Synthesis and characterization of materials Type of data Table  Image Graph Figure  How data were acquired Data were acquired by UV-Vis, FTIR, XRD, universal testing machine, microscope Data format Raw Analyzed Parameters for data collection UV-Vis, FTIR and XRD were collected at room temperature. Universal testing machine was carried out according to the ASTM D882 [2] . Antibacterial activity of biofilms against bacteria was carried using disk diffusion after 24 h incubation.

Value of the data
• Data obtained were important to know the physical, chemical, mechanical and biological properties of biofilm. • Data may be useful for future research on the development of new nano-biocomposite film.
• These data were supported the good performance of fabricated biofilm for antibacterial and cell proliferation.

Data description
The dataset of this article provides information on physical, mechanical, chemical and biological properties of gellan gum incorporating titanium dioxide nanoparticles biofilms. Fig. 1 shows the photo images of pure gellan gum (GG) and gellan gum incorporating TiO 2 nanoparticles (GG + TiO 2 -NPs) biofilms and their UV-Vis spectra were shown in Fig. 2 . The mechanical properties of GG and GG + TiO2-NPs biofilms are presented in Table 1 . Fig. 3 (a) and (b) displays the FTIR spectra and XRD patterns of GG, and GG + TiO2-NPs biofilms as well as TiO 2 nanoparticles (TiO 2 -NPs) powder. Photo images of antibacterial test against gram-positive (Staphylococcus Aureus )   and gram-negative (Escherichia Coli ) bacteria strains using pure GG, GG + TiO 2 -NPs biofilms and penicillin as a control sample were shown in Fig. 4 (a) and (b), respectively. Their antibacterial activity was summarized in Table 2 . Fig. 5 (a) and (b) shows the fluorescence images of the cell viability and cell proliferation, respectively at different time interval.

Experimental design, materials, and methods
Gellan gum incorporating TiO 2 nanoparticles biofilm was fabricated using solvent casting method as used previously by other researchers [ 3 , 4 ]. Firstly, gellan gum solution was prepared by dissolving 1 g gellan gum in 100 mL deionized water under continuous stirring for 2 h at 70 °C. Glycerol (50 w/w%, percentage weight relative to GG) and calcium chloride (CaCl 2 ) (5 mM) was added into the solution. Then, 1 w/w% (percentage weight relative to GG) of TiO 2 nanoparticles was added and stirred for 2 h. The solution was transfer to casting dish and was dried in oven for 24 h at 50 °C to produce spherical shape biofilm. All biofilms were preconditioned in a desiccator (27 °C, 50% relative humidity (RH) for at least 2 days prior to testing. Pure GG biofilm was prepared using similar procedure with the absence of TiO 2 nanoparticles. The obtained samples were characterized using UV-Vis, FTIR, XRD for physical and chemical characterization. The mechanical properties of biofilms were measured using Instron universal testing machine (model 3366) according to ASTM D882 [2] . Five specimens with the size of 2.0 × 6.0 cm rectangular strips of biofilm samples were tested. The biological properties were studied for antibacterial and cell proliferation. Gram-positive ( Staphylococcus aureus ) and Gram-negative ( Escherichia coli ) microbes were used for an anti-bacterial assay. Meanwhile, the cells proliferations were quantified using a MTT (3-(4,5-dimethylthiazol-2-yl) −2,5-diphenyltetrazolium bromide) (Thermo Fisher Scientific, USA). The viability of cells in contact with the biofilm samples for 24 h, 48 h, and 72 h of incubation time was examined through a staining procedure of acridine orange/propidium iodide (AO/PI, Sigma Aldrich, USA) and observed by light microscope (Olympus IX73-FL-CCD). Control in this experiment is Dulbecco's Modified Eagle Medium (DMEM) culture media without the presence of film samples.