Dataset on the shooting and rooting ability of Morus alba using waste tea residue derived carbon dots as an alternative of growth plant stimulator

The data article ex vitro (vegetative plant propagation) culture techniques are sustainable alternatives to the large-scale production of economically important plant species. Morus alba is an essential species that is mainly considered to be economically important due to their potential use as silk production, medicine and food. In this work, we evaluated the data of effects of different concentration of Waste Tea Residue Carbon Dots (WTR-CDs) on the ex vitro growth of morus. This dataset can be beneficial for researchers finding alternative eco-friendly, biodegradable and cost-friendly substitute for plant growth stimulator that are helpful for plant propagation during plant production program. Time consuming and low germination ratio of seeds are the most restricting triggers for commercial use for large-scale cultivation of plant species. Use of WTR-CDs in ex vitro culture technology is an appropriate alternative approach for large-scale production of plants within a short period of time.


a b s t r a c t
The data article ex vitro (vegetative plant propagation) culture techniques are sustainable alternatives to the large-scale production of economically important plant species. Morus alba is an essential species that is mainly considered to be economically important due to their potential use as silk production, medicine and food. In this work, we evaluated the data of effects of different concentration of Waste Tea Residue Carbon Dots (WTR-CDs) on the ex vitro growth of morus. This dataset can be beneficial for researchers finding alternative eco-friendly, biodegradable and costfriendly substitute for plant growth stimulator that are helpful for plant propagation during plant production program. Time consuming and low germination ratio of seeds are the most restricting triggers for commercial use for large-scale cultivation of plant species. Use of WTR-CDs in ex vitro culture technology is an appropriate alternative approach for large-scale production of plants within a short period of time.
© 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).  [2]. Fig. 4 showed the comparison between control and optimized concentration of WTR-CDs as well as statistical analysis data which shown in Table 1. Figs. 5 and 6 discovered the shoot growth length, number of roots and root length of nodal explant of M. alba.

Materials and methods
Mulberry plants (M. alba) were taken from a single mother plant in the Shivaji University, Kolhapur (Maharashtra) campus. Around 60 healthy nodal explants cutting in the form of pencil size diameter Specifications Table   Subject Agronomy Specific subject area Plant propagation or ex vitro. Type of data and 5e6 cm length for plant propagation. Those nodal explants were cut near about 45 angel from the base side of plant stick stem with one bud, Instead, 0.1% (w/v) mercury chloride was used for surface sterilized for 30 min after that treatment rinsing them with sterile distilled water for five times.

Preparation and characterzation of WTR-CDs
The synthesis and characterization of WTR-CDs is discussed in the article: 'Sustainable carbon nanodots synthesized from kitchen derived waste tea residue for highly selective fluorimetric

WTR-CDs treatment
Sterilized nodal explant of M. alba were equally distributed (10 per set) in six set, set II, III, IV, V and VI was dipped in 20 mL of 50, 100, 150, 200 and 250 mg/L WTR-CDs solutions respectively for 7 days in beaker (Fig. 1 a). Similarly another set (I) was dipped in the water (control).

Optimized concentration treatment of WTR-CDs
The plan of the experimentation was a Randomized Complete Block Design (RCBD) where five cuttings of nodal explant were exposed to two treatments (control and optimized concentration of WTR-CDs) and five times replicate. After bud formation, some bud formed M. alba explant samples were subjected to the plantation in the plastic trays. One set was kept in test tube under observation to check the root formation. The following observations data were given at the end of the experiment; Number of bud formation, growth of bud, number of roots and root length of explant.

Statistical analysis
The dataset collected was examined using Analysis of Variance (Single factor ANOVA) and the means were using t-Test: Paired Two Sample for Means.