Dataset of Cavendish banana transcriptome in response to chitosan coating application

Banana is a climacteric fruit and its ripening process is greatly influenced by presence of ethylene. This physiological climacteric characteristic of banana fruit leads to a fast ripening and a short shelf-life. Application of edible coating such as chitosan aims to prolong fruit shelf life. The knowledge on gene expression will help to understand the fruit ripening process itself and chitosan effect on global gene expression. Global gene expression data of chitosan treated and control of Cavendish banana during fruit ripening were provided. Total RNA was isolated from banana pulp for differential gene expression analysis. The RNA-sequencing generated ranged from 16,155,947 to 23,587,110 total reads, with 75.8%–83.8% of reads were mapped against the genome reference. In total, 33,797–35,944 transcripts were detected. The transcriptomics data discussed in this publication are accessible through NCBI's Gene Expression Omnibus with GEO Series accession number GSE139457. These data provide information to identify candidate genes involved in fruit ripening in response to chitosan coating to design a better banana postharvest management.


a b s t r a c t
Banana is a climacteric fruit and its ripening process is greatly influenced by presence of ethylene. This physiological climacteric characteristic of banana fruit leads to a fast ripening and a short shelf-life. Application of edible coating such as chitosan aims to prolong fruit shelf life. The knowledge on gene expression will help to understand the fruit ripening process itself and chitosan effect on global gene expression. Global gene expression data of chitosan treated and control of Cavendish banana during fruit ripening were provided. Total RNA was isolated from banana pulp for differential gene expression analysis. The RNA-sequencing generated ranged from 16,155,947 to 23,587,110 total reads, with 75.8%e83.8% of reads were mapped against the genome reference. In total, 33,797e35,944 transcripts were detected. The transcriptomics data discussed in this publication are accessible through NCBI's Gene Expression Omnibus with GEO Series accession number GSE139457. These data provide information to identify candidate genes involved in fruit ripening in response to chitosan coating to design a better banana postharvest management.
© 2020 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).

Data description
This paper provides transcriptomics data of uncoated (control) and 1.25% chitosan-coated banana fruit to understand the delay in fruit ripening mechanism by chitosan coating. Previous study by Lustriane et al. [1] showed that chitosan was able to delay banana fruit ripening. The changes in global transcriptome during the course of ripening of uncoated and chitosan-coated banana were evaluated in order to obtain a better understanding on the mechanisms involved in ripening delay upon chitosan treatment and applied to design a better postharvest management. The transcriptomics dataset files generated from the 12 sets of Musa acuminata pulp (uncoated and chitosan coated fruit) have been deposited to Gene Expression Omnibus (GEO) NCBI database [2]. The RNA-seq raw data are available at NCBI's Sequence Read Archive (SRA) database. Description of the materials, total RNA extraction, sequencing and transcriptome construction are given in the next section. The transcriptomics dataset statistics could be seen in Tables 1 and 2 Value of the Data These data provide the first information of global gene expression in response to chitosan coating during banana ripening. These data are crucial to identify candidate genes involved in fruit ripening in response to chitosan coating. These data will be useful to design a better management of banana postharvest.

Plant material
Ethylene-treated banana fingers were used as samples for fruit ripening process. Three biological replicates of banana pulp samples were taken on 1st and 7th day from each untreated and 1.25% chitosan treated banana, as previously described by Pratiwi et al. [3], Lustriane et al. [1], Yamamoto et al. [4] and with modification.

RNA isolation
Total RNA was extracted from fruit pulp of each sample using Cordeiro's method [5]. The RNA concentration and quality were examined with NanoDrop spectrophotometer (Eppendorf Bio-Spectrometer® Kinetic) at 230, 260, and 280 as well as electrophoresis on 1.5% agarose gel. DNAseI kit from Thermo Scientific (Catalog Number: EN0521) was then used to purify RNA samples. The cDNA synthesis was then performed using the iScript™ cDNA Synthesis kit (Biorad Catalog Number: 170e8890) in thermal cycler (Applied Biosystem™ Veriti™ 96-Well Fast Thermal Cycler).

RNA library construction and sequencing
The TruSeq RNA Sample Prep KIT v2 was then used to construct RNA library from each sample and sequenced using Ilumina platform HiSeq 2000 with HCS V2.2 software.