Data on cytotoxic and antibacterial activity of synthesized Fe3O4 nanoparticles using Malva sylvestris

The biosynthesis of materials using medicinal plants can be a low-cost and eco-friendly approach due to their extraordinary properties. Herein, we reported a facile synthesis of Fe3O4 nanoparticles using Malva sylvestris. The surface morphology, functional groups, and elemental analysis were done to characterize the synthesized nanoparticles. The cytotoxicity performance of the synthesized nanoparticles was analyzed by exposing nanoparticles to MCF-7 and Hep-G2 cancer cell lines through MTT colorimetric assay and the IC50 value was defined as 100 μg/mL and 200 μg/mL, respectively. The antibacterial performance of synthesized nanoparticles against four different bacterial strains including Staphylococcus aureus, Corynebacterium, Pseudomonas aeruginosa, and Klebsiella pneumoniae were assessed through microdilution broth method. The synthesized Fe3O4 nanoparticles using Malva sylvestris demonstrated higher antibacterial effects against Gram-positive strains with MIC values of 62.5 μg/mL and 125 μg/mL which increase the inhibitory percentage to more than 90%.


a b s t r a c t
The biosynthesis of materials using medicinal plants can be a lowcost and eco-friendly approach due to their extraordinary properties. Herein, we reported a facile synthesis of Fe 3 O 4 nanoparticles using Malva sylvestris. The surface morphology, functional groups, and elemental analysis were done to characterize the synthesized nanoparticles. The cytotoxicity performance of the synthesized nanoparticles was analyzed by exposing nanoparticles to MCF-7 and Hep-G2 cancer cell lines through MTT colorimetric assay and the IC50 value was defined as 100 mg/mL and 200 mg/mL, respectively. The antibacterial performance of synthesized nanoparticles against four different bacterial strains including Staphylococcus aureus, Corynebacterium, Pseudomonas aeruginosa, and Klebsiella pneumoniae were assessed through microdilution broth method. The synthesized Fe 3

Data
The experimental data on cytotoxic and antibacterial activity of synthesized Fe 3 O 4 nanoparticles using Malva sylvestris are reported in this dataset. To validate the successful synthesis of Fe 3 O 4 nanoparticles using Malva sylvestris, Fourier transform infrared spectroscopy (FTIR) was applied to determine the functional groups in the nanoparticles (Fig. 1a). The X-ray powder diffraction (XRD) analysis is depicted in Fig. 1b The data are useful to compare the physicochemical characters, cytotoxic and antibacterial performances of the synthesized nanoparticles and can investigate the reliability of these synthesized nanoparticles in clinical applications. These data are relevant in developmental biology, especially for the understanding of the antibacterial and anticancer role of synthesized Fe 3 O 4 nanoparticles using Malva sylvestris and their usage in therapeutic procedures for human cancers. So, it may be helpful for biology researchers to find a cancer therapy method. The synthesized nanoparticles can be applied as an antibacterial, antiseptic, and chemo-preventive agent in cancer treatment.
using Malva sylvestris against four bacterial strains was analyzed through microdilution broth assay (see Fig. 4). The performance of nanoparticles against selected microorganisms is presented in Table 1.
The raw data source for this dataset is available in the Supplementary section.

Preparation of Fe 3 O 4 /Malva sylvestris nanoparticles
All of the materials utilized in this investigation like Ferrous(ІІ) Sulfate Heptahydrate (FeSO 4 .7H 2 O), Iron(III) Chloride Six hydrate (FeCl 3 .6H 2 O) and Ammonia solution were purchased from Merck Co. (Germany). First, 4.75 g FeCl 3 .6H 2 O, 3.89 g FeSO 4 .7H 2 O, and 320 mL deionized water were poured into a round-bottom flask and stirred for 1 h while the temperature was set to 80 C [1,2]. Then, 0.5 g Malva sylvestris was ultrasonically mixed in 120 mL of deionized water for 30 min and then poured into the previous suspension. The obtained suspension was stirred under 80 C for about 2 h and after that 40 mL NH 3 was gradually added to the suspension [3]. After filtration, the suspension was washed and the pH scale was set on 7 and finally dried in an oven at 100 C for 2 h.

Characterization of nanoparticles
The synthesized nanoparticles were characterized using Fourier-transform infrared spectroscopy (FTIR, Tensor ІІ FT-IR spectroscopy Bruker, Germany) in the region of 400e4000 cm À1 , X-ray diffraction (XRD, Panalytical model X'Pert Pro), scanning electron microscope (SEM, Tescan model Mira III), and EDX (Tescan model S Max detector Mira III).

MTT assay
The cytotoxicity of the synthesized nanoparticles was evaluated on Hep-G2 and MCF-7 cell lines using MTT colorimetric assay. In this method, hydrogen peroxide was considered as the positive control and culture medium was the negative control. Briefly, a certain number of Hep-G2 and MCF-7 cells (10*10 3 ) were placed in each well of a sterile 96-well microplate and incubated in a humidified atmosphere of 5% CO 2 , 95% air at 37 C to reach about 75e90% confluence. Then, 100 mL of the synthesized nanoparticles in a wide range of concentrations was replaced with previous media. Afterward, 25 mL of the MTT 3-(4,5 Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium stock solution (with 4 mg/mL concentration) was transferred into each well and incubated for 4 h in standard condition. The mitochondrial performance of viable cells led to formation of purple formazan crystals, where for dissolving these crystals we applied 100 mL dimethyl sulfoxide (DMSO). In the final step, the absorption of solution was recorded at 570 nm wavelength using a microplate reader (Model 50, Bio-Rad Corp, Hercules, California, USA).

Minimum inhibitory concentrations (MICs) assay
In MICs test, all of the procedures were carried out according to the standards of the Clinical and Laboratory Standards Institute (CLSI) for assessing the antibacterial susceptibility of the synthesized nanoparticles [4]. Briefly, 2-fold serial dilutions of the testing compounds (at a descending concentration from 1000mg/ml to 7.8 mg/mL) and control groups were provided with Brain heart infusion (BHI) in 96-well microplates. Afterward, the microbial concentration was adapted to match the turbidity standard of 0.5 McFarland (OD600: 0.1e0.2) in a way that the concentration of the compounds was 1000 mg/mL in the first wells. The plates were incubated for 24 h at 37 C. Later the optical density was measured at 600 nm by a microplate reader (BioTek, Power Wave XS2). This procedure was done in triplicate.

Minimum Bactericidal Concentrations (MBCs) assay
All the selected microorganisms were cultured overnight in BHI, and then stocks with the concentration of 10 5 e10 6 CFU/mL were prepared for each one. The total of 90 mL of serially diluted concentrations of compounds (from 1000 mg/mL to 7.8 mg/mL) was added to a 96-well micro-plate consisting of 90 mg/mL BHI, then 10 mg/ml of bacteria were added to each cell. Micro-plates were incubated for 24 h at 37 C. Then, 10 mL of each bacterial suspension was added to a newly prepared BHI and incubated for another 24 hours at 37 C to exam bactericidal performance of each compound The lowest concentration of compounds that leads no growth of bacteria was regarded as minimum bactericidal concentration (MBC). This procedure was also repeated three times.