Dataset on effects of perinatal exposure to propylthiouracil on serum T4, body weight gain, day of eye opening and brain monoamine contents in offspring mice

Physical growth and brain development need sufficient thyroid hormone (TH). This dataset describes serum T4 level, body weight gain and day of eye opening in offspring mice exposed to the TH synthesis inhibitor propylthiouracil (PTU) from gestational day (GD) 15 to postnatal day (PND) 25. This dataset also describes content of dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3-methoxytyramine (3-MT), 5-HT, and 5-hydroxyindoleacetic acid (5-HIAA) and their turnover in the hypothalamus, hippocampus, and nucleus accumbens of male offspring mice perinatally exposed to PTU. These data are referred by a research article entitled “Hyperactive behavioral phenotypes and an altered brain monoaminergic state in male offspring mice with perinatal hypothyroidism” [1].


Data
describes serum T4 level on PND25 in dams, male and female offspring mice given tap water or 125, 250, or 500 ppm PTU solution from GD 15 to PND 25. Fig. 2 shows body weight gain and the day of eye opening in offspring mice given tap water or 125, 250, or 500 ppm PTU solution from GD 15 to PND. Fig. 3, Fig. 4 and Fig. 5 describes content of DA, DOPAC, HVA, 3-MT, 5-HT, and 5-HIAA and their turnover in the nucleus accumbens, hypothalamus, and hippocampus, respectively, of male offspring mice given tap water or 125, 250, or 500 ppm PTU solution from GD 15 to PND 25. Ambulatory activity and content of DA, DOPAC, HVA, 3-MT, 5-HT, and 5-HIAA and their turnover in the striatum of the same male offspring mice are described in the related article [1]. Row data for Figs. 3, Fig. 4, Fig. 5 are described in Table 1, Table 2 and Table 3, respectively. Specifications Table   Subject Toxicology Specific subject area Development of brain monoaminergic system and hypothyroidism Type of data  Figures and Tables  How data were acquired Observation and body weight measurement Radioimmunoassay using DPC・total T4 kits (Yatoron Co., Ltd. (present: LSI Medience Co., Ltd.), Tokyo, Japan) High performance liquid chromatography system (L-5000; Yanaco, Kyoto, Japan) with a 4.6 mm Â 250 mm ODS-C18 column (Nacalai, Kyoto, Japan) and an electrochemical detector (VMD-101A; Yanaco) including a glassy carbon electrode. Data format Raw Parameters for data collection 125, 250, or 500 ppm PTU solution was administered to dams from GD 15 to PND25. Tap water was given to control dams. Tap water was given to all offspring mice thereafter. Every 3e4 days after delivery, the body weight and eye opening of offspring were checked. Blood samples for T4 radioimmunoassay were collected from dams and offspring on PND25. Brain samples for monoamine assay were obtained from male offspring on PND60. Description of data collection Body weight gain data was obtained by measuring every 3e4 days. Value of the Data The data show that perinatal exposure to thyroid hormone synthesis inhibitor propylthiouracil retards growth and affects development of brain monoaminergic system in offspring mice. Data could be valuable for researches on impact of perinatal hypothyroidism on brain monoaminergic system development.
Data could be useful for researchers to further study roles of thyroid hormone for development of brain monoaminergic system. Data could be used by other researchers to study neurodevelopmental effects of chemicals that disrupt thyroid hormone system (thyroid hormone disruptors). Data could be used by other researchers to study central mechanisms underlie mental problems produced by perinatal hypothyroidism. 2. Experimental design, materials, and methods

Animals
ICR (or CD-1) strain mice were used.

Drug
Propylthiouracil (6-Propyl-2-thiouracil or 2,3-Dihydro-6-propyl-2-thioxo-4(1H)-pyrimidinone; PTU) solution at concentrations of 125, 250, or 500 ppm was given as drinking water to pregnant females. The day when a plug was observed in the dam was defined as GD0. Administration of 125, 250, or 500 ppm PTU solution was started on GD15. Tap water was given to control dams. The solution was available ad libitum during the exposure period. The day when delivery was observed was defined as PND0. On PND0, body weight of offspring were measured, followed by measuring the body weight and checking eye opening of offspring every 3e4 days. The offspring mice were weaned, male and female offspring were separated, and exposure to PTU was terminated on PND25. Tap water was given to all offspring mice thereafter. On PND25, the body weight of offspring was measured, followed by measurement of the body weight every 3e4 days.

Blood collection for the serum T4 assay
On PND25, blood collection for the serum T4 measurements was performed. Blood was transcardially collected under deep anesthesia form dams and one or two male and female offspring randomly selected from each litter. The serum samples of the collected blood were stored at À80 C and subjected to T4 measurement at a later time.

Brain monoamine content measurement
The male offspring mice were sacrificed by decapitation on PND60, and the brain was removed. The brain was placed on ice, and the nucleus accumbens, hypothalamus, and hippocampus were bilaterally collected, and frozen in liquid nitrogen. The tissue were kept at À135 C and later subjected to the monoamine content measurement.

Serum T4 assay
Serum T4 levels in the collected blood samples were measured by radioimmunoassay using DPC・ total T4 kits (Yatoron Co., Ltd. (present: LSI Medience Co., Ltd.), Tokyo, Japan) according to the company's instructions.