Structural and antitumoral characteristic dataset of the chitosan based magnetic nanocomposite

The evaluation on the characteristic dataset and figures presented here, are related to our latest research data entitled “Fabrication of chitosan based magnetic nanocomposite by click reaction strategy; evaluation of nanometric and Cytotoxic characteristics” [1]. FTIR, Vibrating Sample Magnetometer (VSM) measurements, Xray diffraction (XRD) information and the resulted figures for structural confirmation of the prepared chitosan based nanocomposite are presented in this article. The morphological changes of the Fibroblast, Saos, MCF7 and Hela cell lines after treatment with the mention compound were displayed. The additional adsorption data for the synthesized nanobiocomposite were also demonstrated with graphs.


Data
IR spectra were recorded on a Perkin-Elmer FT-IR-1710 spectrophotometer with the samples in KBr pellets. Fig. 1 displays the FT-IR spectra of the prepared compounds and the characteristic peaks data were introduced as Table 1. Vibrating Sample Magnetometer (VSM) measurements were performed by using a vibrating sample magnetometer (LDJ Electronics Inc., Model 9600) and the data was inserted as Table 2 and the resulted pattern was displayed in Fig. 2. The X-ray powder diffraction (XRD) of the catalyst was carried out on a Philips PW 1830 X-ray diffractometer with CuKa source (l ¼ 1.5418 Å) in a range of Bragg's angles (5e80 ) at room temperature and demonstrated in Fig. 3. The crystal planes of Fe 3 O 4 which confirm the existence of magnetic nanoparticles in the composite were assigned in Table 3 BrunauereEmmetteTeller (BET) analysis were performed using automatic sorption analyzer ASAP 2020, Micromeritics, USA. The Isotherm Linear Absolute Plot and isotherm Pressure Composition Specifications Table   Subject area Chemistry, Biology More specific subject area Preparation of chitosan based nanobiocomposite Type of data raw data, graph, figure How data was acquired The outcomes were provided by IR, VSM, XRD and MTT assay. Also some descriptions about the composite preparation and images of the morphology of cell lines were presented.

Data format
Raw, analyzed Experimental factors FTIR of the prepared samples, VSM and XRD of the composite, also the images of cell lines were apprised.

Experimental features
The nanocomposite was prepared and characterized using FTIR and imaged by SEM and TEM technique. Then thermophysical experiments were performed using DSC and TGA protocols. Biological characteristics were evaluated with MTT assay and the morphological effects were imaged by microscopic technique.

Data source location
Babol university of medical sciences, Mazandaran, Iran Data accessibility Available in this article Related research article Fabrication of chitosan based magnetic nanocomposite by click reaction strategy; evaluation of nanometric and Cytotoxic characteristics [1] Value of the Data This data presents the structural and physical characteristics of the synthesized biocomposite, from which researchers who are interested in preparation of novel chitosan based nanocomposite especially in medical field can take advantage of it. The isotherm linear absolute and isotherm pressure composition plots data which is introduced as tables and figure can gain the attention of the chemical and environmental engineers for production of the new class of bioadsorbents. The biocharacteristics of the prepared composite toward Fibroblast, MCF7, Hela, and Saos cell lines were investigated and the data can encourage researches towards assessments against other cancer cell typs.

Experimental design, materials and methods
The magnetic nanocomposite was prepared using chitosan. To prepare functionalized chitosan, the chitosan was azidated using chloroacetyl chloride and sodium azide. Then click reaction which has been incorporated in our recent studies [2,3] and also employed in some biological researches [4,5] was performed between functionalized chitosan and trimethoxy(3-(prop-2-yn-1-ylthio)propyl)silane. Then magnetization was done using ferric and ferrous chloride solution. The characteristic peaks for azidated at around 2100 cm À1 , CeH bond of triazole rings and SieOeSi bonds at 3265 cm À1 and 1150 cm À1 respectively. The resulted FT-IR spectra and the corresponding data of the synthesized products were presented in Fig. 1 and Table 1. Also, the detailed FT-IR data including the transmittances at each wavenumbers for the compounds a, b, and c were provided as a supplementary file. Magnetization experiments of the prepared magnetic nanocomposite (MNC) were obtained using VSM technique at room temperature. As can be seen in Fig. 2, this product with saturation magnetization value (M s ) of 25.4 (emu/g) has super paramagnetic characteristics. Moreover; the corresponding data were presented in Table 2.
The XRD pattern of the synthesized chitosan nanocomposite (Fig. 3) demonstrated the crosslinking reaction between Si groups and chitosan with the broad peak at 15e30 . Moreover the existence of magnetic nanoparticles in the structure was confirmed by determining the crystal planes of Fe 3 O 4 nanoparticles (Table 3).  To attain adsorption data of the synthesized nanocomposite for further experiments, the samples were outgassed at 60 C and then experiments according to the BrunauereEmmetteTeller (BET) theory were performed. The isotherm plots were used to calculate the specific surface area and the average pore diameter of the chitosan/magnetic nanocomposite and the difference between adsorption and desorption steps.
For evaluating the cell cytotoxicity of the prepared sample (MNC) according to the literature [6], some known cell lines were considered including Fibroblast, MCF7, Hela, and Saos. The resulted data were surveyed in the main article and the morphology of the cell lines with treatment of different concentrations of the samples were imaged by microscopic technique and presented here in Fig. 5.