Data of insecticide effects of natural compounds against third instar larvae of Cochliomyia macellaria

Morphological biomarkers can be used to establish a diagnosis of fly larvae structural damage and toxicity to target cells by biopesticide candidates. Insecticide activity of natural compounds such as Curcuma longa essential oil (CLLEO) extracted from leaves, and its major constituent α-phellandrene have proven to be a novel biopesticide candidate against third instar larvae (L3) of Cochliomyia macellaria. In this way, groups of 20 L3 were placed on filter paper impregnated with different concentrations of CLLEO, from 0.31 to 2.86 μL/cm2 and α-phellandrene, from 0.29 to 1.47 μL/cm2. The extracts were solubilized in ethanol. Data shown in this article is related to the research article “Can an overlooked by-product from turmeric industry be effective for myiasis control?” Chaaban et al., 2019. Data on L3 toxicity was observed after 6 and 24h of contact with both extracts, as well as a marked reduction of L3 movement, color changes in the cuticle and progressive darkening in their body. Major cuticle damage and L3 mortality were reported.


a b s t r a c t
Morphological biomarkers can be used to establish a diagnosis of fly larvae structural damage and toxicity to target cells by biopesticide candidates. Insecticide activity of natural compounds such as Curcuma longa essential oil (CLLEO) extracted from leaves, and its major constituent a-phellandrene have proven to be a novel biopesticide candidate against third instar larvae (L3) of Cochliomyia macellaria. In this way, groups of 20 L3 were placed on filter paper impregnated with different concentrations of CLLEO, from 0.31 to 2.86 mL/cm 2 and a-phellandrene, from 0.29 to 1.47 mL/ cm 2 . The extracts were solubilized in ethanol. Data shown in this article is related to the research article "Can an overlooked byproduct from turmeric industry be effective for myiasis control?" Chaaban et al., 2019. Data on L3 toxicity was observed after 6 and 24h of contact with both extracts, as well as a marked reduction of L3 movement, color changes in the cuticle and progressive darkening in their body. Major cuticle damage and L3 mortality were reported.
© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).

Data
The data of this paper involves the experimental analysis regarding the cuticular damage of the natural compounds CLLEO, and its major constituent a-phellandrene against L3 of C. macellaria [1]. L3 from the control group, showed no change in cuticle morphology after 6 and 24h of contact (Video 1a, 1b; Video 2a, 2b). Data of the insecticide effect of the extracts was observed a few hours after contact with the doses of 1.59 and 1.47 mL/cm 2 of CLLEO and a-phellandrene, respectively (Video 1c; Video 2c).
Specifications Fresh aerial parts of Curcuma longa leaves, a by-product from turmeric, and its major compound a-phellandrene were assessed for insecticidal activity over Cochliomyia macellaria. The assays were performed as described in the companion paper "Can an overlooked by-product from turmeric industry be effective for myiasis control?" [1] Experimental features CLLEO extraction and chemical characterization. Establishment of C. macellaria colonies for biological assays on laboratory conditions (27 ± 2 C and 70% relative humidity).
Contact tests using filter paper impregnated with CLLEO and its major compound a-

Value of the data
Research data highlights the insecticide activity of Curcuma longa (Leaves) essential oil, and its is major compound aphellandrene against third instar larvae of Cochliomyia macellaria, which is a common myiasis infection in livestock. The data of the use of the by-product from turmeric, as an ecofriendly bioinsecticide highlights a novel natural alternative, which may be beneficial to the welfare of animals and local economies.
The essential oil extracted from the leaves of Curcuma longa and a-phellandrene demonstrates the possible development of a potent insecticide compound. The data provide a valuable reference for future data collection using the above biopesticide even against other insects that affect livestock and humans.
Moreover, progressive darkening in L3 body, marked reduction of larval movement, color changes in L3 cuticle and death were observed after treatment (Video 1d; Video 2d).
Supplementary video related to this article can be found at https://doi.org/10.1016/j.dib.2019. and certified as having 99% purity.

Establishment of Cochliomyia macellaria colonies and larval toxicity
Data of the establishment of stock colonies, insects' identification, mass reproduction, and the protocol for the biological tests were performed as described in the companion paper [1]. The toxicity of CLLEO and a-phellandrene over L3 of C. macellaria was performed using groups of 20 L3, which were placed on filter paper that were impregnated with a range of concentrations of CLLEO (0.31e2.86 mL/ cm 2 ) and a-phellandrene (0.29e1.47 mL/cm 2 ). L3 were put into glass vials containing filter papers (12.56 cm 2 ) impregnated with 0.2 mL of EO solution, that were solubilized in ethanol using the protocol described by Chaaban et al. (2017) [2]. The toxicity was evaluated by observing L3 mortality at 6, 24 and 48 h after contact [2,3]. Total larval mortality (LM) was calculated [2e4] as follows: LM ¼ (total dead larvae x 100) / total tested larvae Damages were measured by macroscopic biomarker changes and microscopic lesions using histological sections and scanning electron microscopy in L3 treated with 1.59 mL/cm 2 of CLLEO and 1.47 mL/cm 2 of a-phellandrene, both solubilized in ethanol. The data on these alterations can be observed in the companion paper [1].