The soybean root membrane lipids and forage quality data in response to field cultivation on agricultural podzols in boreal climates

The objective of this data in brief article is to represent the associated data set regarding our published paper in Plant Science Nadeem et al., 2019. Data set represent soil acid phosphatase activity, association of individual molecular species of four major lipid classes with soybean forage quality indices when cultivated in boreal podzolic soils under cool climatic conditions. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) and acylated glucosyl betasitosterol ester (AGIcSiE) molecular species grouped the soybean forage quality indices in different quadrants on principal component analyses. Furthermore, the total lipid profile and correlation of major lipid species with forage quality indices are included in this data in brief article. This data set support the main findings described in Nadeem et al., 2019.

quality indices are included in this data in brief article. This data set support the main findings described in Nadeem

Data
This data set contain information regarding soybean root lipids, forage quality indices and correlations among these indices when soybean forage are cultivated at 3 farm sites across Newfoundland containing agricultural podzols with varying soil pH. Data set contains five figures and two tables demonstrating the relationship among lipid molecular species and soybean forage quality indices. Figure 1 described the soil acid phosphatase activity in soybean roots rhizosphere across three farm sites. Fig. 2(ab) demonstrates the relationships between root membrane phosphatidylethanolamine (PE) molecular species and forage soybean nutritional quality following cultivation in acidic soil in cool climate production system. The third figure (Fig. 3ab)  Value of the data Multidisciplinary data set obtained from three farm locations across Newfoundland containing podzolic soils with varying pH levels Data set involves biochemistry, dairy science and agriculture which demonstrate the importance of root lipid in producing higher quality forage on podzolic soils in cool climates of boreal ecosystem Data set depict the importance of membrane lipid remodeling for sustainable crop production on podzolic soils in boreal ecosystem as part of a strategy to improve food security in the Boreal or northern regions Data set demonstrates similar result trends and add more context to the results observed in the research article by Nadeem et al., [1]. membrane phosphatidylcholine (PC) molecular species and forage soybean nutritional quality following cultivation in acidic soil in cool climate production system. The fourth figure (Fig. 4ab) shows the relationships between root membrane phosphatidic acid (PA) molecular species and forage soybean nutritional quality following cultivation in acidic soil in cool climate production system. The fifth figure (Fig. 5ab) demonstrates the relationships between root membrane acylated glucosyl betasitosterol ester (AGIcSiE) molecular species and forage soybean nutritional quality following the cultivation in acidic soil in cool climate production system. Table 1 depicts the percent fold changes in root lipid profile compared to neutral pH (6.8) along with three lipid classes. Table 2 shows the correlation among four major lipid species (PE, PA, PC, AGIcSiE) and forage quality indices.

Experimental design, materials and methods
Experimental design, materials and methods were based on research article Nadeem et al., [1], whereas soil phosphatase activity assay is described below.

Soil phosphatase activity
A half of the soil samples collected from the root rhizosphere was used to determine the soil acid phosphatase activity according to the modified methods of Tabatabai and Bremner [2]. Briefly, a 1 g of 2 mm sieved soil sample was weighed and extracted with 1 mL of 0.09 M citrate buffer with 4.8 pH. Polypropylene centrifuge tubes containing soil and citrate buffer were then centrifuged (Heraeus™ Megafuge™ 16 Centrifuge Series) at 5000Âg for 10 min. A 50 mL aliquot of the supernatant was collected and the soil acid phosphatase activity was assessed after incubating for 30 min in an oven (Shel Lab FX14-2, Sheldon Manufacturing Inc. USA) at 37 C with 1 mM 4-nitrophenyl phosphate and 50 mL citrate buffer. The reaction was terminated immediately after incubation with 20 mL of 0.5 N NaOH. The absorbance was recorded at 405 nm using a spectrophotometer (BioTek Cytation 3 Imaging Reader, USA) and the final enzyme activity presented as mmol pNP g À1 of soil per 30 min.      Table 1 The effect of soil acidity (pH 6.0 and 5.1) on root membrane lipidome percent fold changes compared to neutral soil pH (6.8) in forage soybeans grown under cool climatic conditions. Values represent the percent fold changes in lipids compared to neutral soil pH (6.8). Lipid class separation was done using hydrophilic interaction chromatography coupled to a high-resolution Orbitrap mass spectrometry.
Values of lipid classes were marked as bold.

Table 2
Pearson's correlation between four major lipid species and soybean forage production, and forage quality grown on three agricultural farms with different soil pH level under cool climatic production system in Newfoundland, Canada.  Ns: non-significant, * significant at alpha 0.05, ** at 0.01, and *** at 0.001.
Higher correlation values were marked as bold, whereas, bold and italic values were marked significant.