Data on association of the variation (rs1344706) in the ZNF804A gene with schizophrenia and its symptoms in the Russian population

The polymorphism rs1344706 in the ZNF804A gene is one of the best-supported risk variants for schizophrenia. The association between ZNF804A rs1344706 and the disease was demonstrated in many studies but only few of them investigated large samples (above 2000 patients and controls). Data presented show the genotypic distribution of ZNF804A rs1344706 in 1265 patients with schizophrenia and 1051 healthy controls from the Russian population. Statistical analysis confirmed the association between rs1344706 and schizophrenia (p = 0.034). The frequency of the risk genotype AA was significantly higher in the group of patients compared to that in controls. In addition, the article provides the data on the severity of schizophrenia symptoms measured with the Positive and Negative Syndrome scale (PANSS) in 951 patients. The severity of symptoms was significantly higher in the carriers of the risk genotype AA compared to the AC genotype and the CC genotype.


a b s t r a c t
The polymorphism rs1344706 in the ZNF804A gene is one of the best-supported risk variants for schizophrenia. The association between ZNF804A rs1344706 and the disease was demonstrated in many studies but only few of them investigated large samples (above 2000 patients and controls). Data presented show the genotypic distribution of ZNF804A rs1344706 in 1265 patients with schizophrenia and 1051 healthy controls from the Russian population. Statistical analysis confirmed the association between rs1344706 and schizophrenia (p ¼ 0.034). The frequency of the risk genotype AA was significantly higher in the group of patients compared to that in controls. In addition, the article provides the data on the severity of schizophrenia symptoms measured with the Positive and Negative Syndrome scale (PANSS) in 951 patients. The severity of symptoms was significantly higher in the carriers of the risk genotype AA compared to the AC genotype and the CC genotype.
© 2019 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons. org/licenses/by/4.0/).

Data
The dataset reports the frequencies of alleles and genotypes of ZNF804A rs1344706 determined in patients with schizophrenia and healthy people from the Russian population (Table 1). Table 2 describes demographic characteristics in carriers of different ZNF804A rs1344706 genotypes. Table 3 contains the data on clinical characteristics (age at disease onset and symptom scores) stratified by ZNF804A rs1344706 genotype. Table 4 shows the clinical characteristics of male and female patients with different ZNF804A rs1344706 genotypes. The distribution of genotypes is different for patients and controls (Chi-square ¼ 6.71, df ¼ 2, p ¼ 0.034). The frequency of the risk genotype AA is higher in the group of patients compared to that in controls (p ¼ 0.01). This finding is consistent with other studies [1,2]. There are no between-genotype differences in age and sex in both groups. ANOVA reveals the effect of sex on the age at disease onset and scores on PANSS positive, negative, general psychopathology subscales (p < 0.05) but not on the total PANSS score. Male patients have younger age at disease onset, lower positive symptom score and higher scores on negative and general psychopathological subscales. The main effect of the ZNF804A rs1344706 genotype on the scores on PANSS subscales and the total PANSS score is shown (p < 0.05). The severity of symptoms is higher in the carriers Specifications Value of the data The data can be used in meta-analyses of clinical and molecular-genetic studies of schizophrenia. Allele and genotype frequencies for ZNF804A rs1344706 in the Russian population can help in population genetic studies. The data on sex-differences in clinical characteristics of schizophrenia are valuable for epidemiological studies of schizophrenia.
Replication of the association between ZNF804A rs1344706 and schizophrenia in the large samples of patients and controls confirmed the contribution of the AA genotype to the risk of schizophrenia. The data on the impact of ZNF804A rs1344706 variants on the severity of symptoms of schizophrenia are important for prediction of disease outcomes.

Sample collection and genomic DNA extraction
Schizophrenia sample was selected from patients admitted to psychiatric units of the Mental Health Research Center. The sample included 1265 people, 550 men, 715 women, mean age 34.8 (13.7) years. Healthy controls without a family history of mental diseases were enrolled from Moscow and Moscow region. The control group included 1051 people, 557 men, 494 women, mean age 30.5 (11.4) years. All participants were ethnically Russian. All of them provided the written informed consent for participation in the study. DNA from whole blood was extracted using the standard phenol-chloroform method.

Phenotyping
The diagnosis of schizophrenia was made according to criteria of The International classification of Diseases 10th revision (ICD-10). Clinical symptoms were measured using the Positive and Negative  Syndromes Scale (PANSS) [6], a widespread instrument proven to be valid and suitable for evaluation of positive, negative and general psychopathological items. The PANSS interviews were conducted one week before the patient's discharge from the hospital. Because sex-modulated association of ZNF804A with schizophrenia [7,8] was reported in earlier studies, we additionally considered clinical characteristics separately in men and women.

Statistical genetic analyses
Allele and genotype frequencies in cases and controls were compared using 2 chi-square contingency tables. ANCOVA with Bonferroni post-hoc test was used with PANSS score or age at onset as a dependent variable and genotype (AA vs AC vs CC) and sex as between-subject factors with age as a covariate.