Data on localization of coxsackievirus and adenovirus receptor (CAR) in prenatal and adult rat olfactory, intestine, pancreas, liver, ovary, and testis

The data in the present article are related to the previous article entitled “Coxsackievirus and adenovirus receptor-positive cells compose the putative stem/progenitor cell niches in the marginal cell layer and parenchyma of the rat anterior pituitary” (M. Chen et al. 2013). The data describe the characteristic localization in the immature cells of the prenatal and adult tissues beyond the germ layer. Germ cells and the reproductive tissues of both sexes showed distinct intracellular polarities of CAR: apical, basolateral, and pericellular in the immature cells of the embryo and adult tissues. In addition, the data describe on localization of CAR in the methimazole-induced damage of olfactory epithelium tissue. The data show that the CAR-_immuno-positive cells at the apical side of the olfactory epithelium disappeared following methimazole treatment and reappeared in the regenerating stem/progenitor cells (positive for KI67 and E-cadherin) of the basal layer with basolateral expression.


Data
Data on immunohistochemistry for coxsackievirus and adenovirus receptor (CAR), which plays multifold functions [1e5], show its localization in several rat tissues beyond the germ layer as summarized in Table 1. Images of the prenatal olfactory area originating from the surface epithelium of the ectoderm show coexistence with stem/progenitor markers, E-cadherin and SOX2, and with a cell division marker, KI67, in some of the CAR/SOX2-double positive cells (Fig. 1).
Data on the endodermally-derived tissues, such as the intestine, pancreas, and liver, show CARpositive signals with apical, basolateral, and pericellular polarities and coexistence with E-cadherin (Figs. 2e4).
Data on the mesodermally-derived adult tissues show that CAR was positive in the developing immature, primary, secondary, and tertiary vesicular follicles, except for atretic follicle (Fig. 5f), and in the pericellular of oocytes and pellucid zone of the Graafian follicle (Fig. 5e). CAR-signals are observed with polarized locations along with E-cadherin in the three ovarian surface epithelial cell types ( Fig. 5c''', d''', f''') covering the ovary containing undifferentiating cells [6,7]. Data on the adult testis showed stage-dependent localization of CAR during spermatogenesis (Fig. 6aej) and in the testicular interstitium, some of the mesenchymal cells and Leydig cells (Fig. 6kem). Acrosome of spermatozoa in the epididymis was positive for CAR ( Fig. 6oep").
Data on CAR after methimazole treatment to damage the olfactory mucosa [8] and to regenerate with neural crest-derived stem cells [9] show the disappearance of the apically CAR-positive cell layer and the appearance of a basolaterally CAR-positive layer of the stem/progenitor cells positive for SOX2, KI67, and E-cadherin (Figs. 7 and 8).

Subject area
Biology More specific subject area Developmental biology Type of data Immunohistochemistry How data was acquired Immunofluorescence images were obtained by fluorescence microscopy (Keyence BZ-9000).

Data format
Tables, figures Experimental factors Immunohistochemistry was performed for CAR, the cell division marker KI67,and the stem/ progenitor cell markers SOX2 and E-cadherin.

Experimental features
Tissues were prepared from prenatal and adult rats and methimazole-induced olfactorydamaged rats. Sections were fixed with 4% (w/v) paraformaldehyde. After immuno-reaction with primary antibodies, fluorescein isothiocyanate-, Cy3-, or Cy5-conjugated secondary antibodies were used for detection.

Data source location
Kawasaki, Kanagawa, Japan Data accessibility Data are within the present article

Value of the data
The data would provide a platform to further explore and understand the role of a common receptor for coxsackievirus and adenovirus (CAR) in tissue development.
The data on the localization of CAR in the cells of the prenatal and adult rat tissues beyond the germ layer can be useful for researchers interested in tissue differentiation and development.
The data on the apical and basolateral localization of CAR in differentiating tissues can be useful for investigators interested in molecular mechanism of stem/progenitor cell function.
The present data that CAR-and E-cadherin-expressing stem/progenitor cells are involved in the regeneration of the olfactory epithelium following methimazole-induced damage could be valuable in understanding olfactory epithelial regeneration.

Animals
Male Wistar-Imamichi strain rats were used. Breeding of rats and sampling of brains are described in the previous papers [1]. The present experimental design was approved by the Institutional Animal Care and Use Committee, Meiji University and was performed in accordance with the NIH Guidelines for the Care and Use of Laboratory Animals.
Lesions in the olfactory epithelium were induced according to a previously published method [10]. Methimazole (63760 Fluka, SigmaeAldrich, Saint Louis, MO, USA) was diluted at 5 mg/ml in 0.9% NaCl and injected intraperitoneally into 12-week-old rats at a concentration of 50 mg/kg of body weight. The rats were euthanized at 1 day after the methimazole injection. Rats were sacrificed by exsanguination from the right atrium under deep pentobarbital anesthesia (40 mg/kg) and then perfused with 4% paraformaldehyde in 0.02 M HEPES buffer (pH 7.4) for experiments.          Fig. 7, except for e, h, k, and n (Cy5, white, image for E-cadherin). Boxed areas in aec and gei are enlarged in def and jel, respectively. Scale bars, 50 mm.

Immunohistochemistry
Procedures of an antigen retrieval, fixation, and immunostaining were performed as described previously [1] using primary antibodies and secondary antibodies under the conditions listed in Table 2.