Data on chemical composition of alkaloids of Plumula nelumbinis and antioxidant activity from thirteen habitats in China

Plumula nelumbinis is widely consumed as tea for its pharmacological properties, which is related to its chemical composition, so the identification of the major compounds of P. nelumbinis is valuable. The data described in this article is supported by the research article entitled “Chemical composition of alkaloids of Plumula nelumbinis and their antioxidant activity from different habitats in China” (Tian et al., 2018). Included are the MS-MS Spectrograms of seven alkaloid standards and thirty alkaloids identified in the P. nelumbinis, which is based on ultra-performance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry method. Also included are the total alkaloids content and the antioxidant activity of total alkaloid in P. nelumbinis from 13 habitats in China, which was accomplished with three different antioxidant assays.


a b s t r a c t
Plumula nelumbinis is widely consumed as tea for its pharmacological properties, which is related to its chemical composition, so the identification of the major compounds of P. nelumbinis is valuable. The data described in this article is supported by the research article entitled "Chemical composition of alkaloids of Plumula nelumbinis and their antioxidant activity from different habitats in China" (Tian et al., 2018). Included are the MS-MS Spectrograms of seven alkaloid standards and thirty alkaloids identified in the P. nelumbinis, which is based on ultraperformance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry method. Also included are the total alkaloids content and the antioxidant activity of total alkaloid in P. nelumbinis from 13 habitats in China, which was accomplished with three different antioxidant assays. &

Value of the data
Method and data can be used to identify natural alkaloids by UPLC-ESI-QTOF-MS. The chromatographic and mass spectrometric data can be used for comparison with other studies performed on Plumula nelumbinis, then serve as a benchmark for other researchers to elucidate the constituents of P. nelumbinis.
The alkaloid contents and the antioxidant activity data will provide a valuable reference for studies comparing the chemical and pharmacological effects of P. nelumbinis.

Data
The structures and MS-MS spectrograms of seven alkaloid standards is provided in Fig. 1. Data in Fig. 2 presents the MS-MS spectrograms of 30 alkaloids identified in the Plumula nelumbinis from Xiangtan, Hunan province. Data in Table 1 includes the total alkaloids content and antioxidant capacity of P. nelumbinis obtained from 13 different habitats in China.

Extraction and isolation
Dried P. nelumbinis was extracted three time with 80% EtOH-H 2 O, and the ethonal extract isolated by D001 cation exchange resin chromatography, as washed with water and 95% ethanol (included 1% ammonia) in turn, the 95% ethanol/ammonia wash fraction contains the bulk of the alkaloids, which was stored at À20°C after freeze-drying. The detail procedures were described in Ref. [1].

UPLC-ESI-QTOF-MS parameter
Identification of the samples were carried out using a Shimadzu Prominence UPLC system (Nexera UHPLC LC-30A, Kyoto, Japan) equipped with a A triple TOF TM 5600 þ mass spectrometer (AB Sciex, Foster City, CA). And the detail parameter setting of LC system and mass spectrometric detection were selected as described in Ref. [1]. Briefly, the analysis was carried out using a Waters Acquity UPLC BEH C 18 column (2.1 mm Â 100 mm, 1.7 mm) and the mobile phase of acetonitrile (containing 0.1% formic acid)-water (containing 0.1% formic acid) at a flow rate of 0.3 mL/min with the column temperature kept at 40°C and the S1. Liensinine S2. Isoliensinine injection volume of 3 mL. And the mass spectrometric detector was operated in the positive ESI mode and was equipped with a DuoSpray TM source (AB Sciex, Foster City, CA).

Total alkaloid content
The total alkaloid content of P. nelumbinis was determined using the acid dye colorimetric method according to the methods in Ref. [1]. In which, neferine was used as a reference compound.

Antioxidant activity of total alkaloids
In this study, three different chemical methods, like the DPPH, ORAC and FRAP assays were used to evaluated the antioxidant activity of the total alkaloids which from different habitats.
The free radical-scavenging activity of the total alkaloids of P. nelumbinis was determined with a DPPH test according to the methods in Ref. [1].
The ORAC assay was completed in accordance with the previously described according to the methods in Ref. [1].  The Ferric reducing antioxidant power assay of P. nelumbinis were determined according to the methods in Ref. [1].   Table 1 Total flavonoids content and antioxidant capacity of Plumula nelumbinis obtained from different habitats, cited on a dry weight basis.