Dataset on seed details of wheat genotypes, solution treatments to measure seedling emergence force and the relation between seedling force and strain

The seed details (weight, vigor) and germination rate of 16 wheat (Triticum aestivum) genotypes in a non-limiting conditions were measured. The dataset presents seed germination rate and seed vigor of 16 wheat genotypes. The dataset also presents the concentrations of the cations to create solution treatments of various sodium adsorption ratio (SAR) and ionic strength (I). Finally, dataset presented a figure of the experimental design to measure seedling emergence force of wheat genotypes. The image of the setup and the relation between strain and force have been presented here to convert the strain of the beam into seedling emergence force. This dataset has been used in research work titled ‘Greater emergence force and hypocotyl cross sectional area may improve wheat seedling emergence in sodic conditions’ (Anzooman et al., 2018) [1].


a b s t r a c t
The seed details (weight, vigor) and germination rate of 16 wheat (Triticum aestivum) genotypes in a non-limiting conditions were measured. The dataset presents seed germination rate and seed vigor of 16 wheat genotypes. The dataset also presents the concentrations of the cations to create solution treatments of various sodium adsorption ratio (SAR) and ionic strength (I). Finally, dataset presented a figure of the experimental design to measure seedling emergence force of wheat genotypes. The image of the setup and the relation between strain and force have been presented here to convert the strain of the beam into seedling emergence force. This dataset has been used in research work titled 'Greater emergence force and hypocotyl cross sectional area may improve wheat seedling emergence in sodic conditions' (

Value of the data
The dataset presented in this article can be used to investigate seed quality (seed germination rate, seed size and weight) of 16 wheat genotypes.
The dataset represented here can be used to determine the solution concentrations of sodium chloride (NaCl) and calcium chloride (CaCl 2 Á 2H 2 O) to create solutions of different SAR.
The dataset can be used to measure seedling emergence force of wheat or other crop genotypes.

Data
This paper represents the dataset which have been used to investigate seed quality and germination rate of 16 wheat genotypes. Seed width and length was measured of these genotypes using slide calipers (Table 1) from 50 replicates of each genotype. The numbers inside the brackets in Table 1 represents the standard error between the genotypes. To create solutions of four different SAR (0, 10, 40 and 60) and four different I (25, 50, 300, 484 mmol L À 1 ) different concentration of NaCl and CaCl 2 were used which had been described in Table 2. A device was developed to measure the seedling emergence force of these 16 genotypes which had been shown in Fig. 1. This machine measured the deflection of a steel beam place on top of a foam when the seedling emerged from the foam and moved the beam. The deflection of the beam was measured using the stain gaze connected to the beam. The measured strain was converted to force using the calibration graph (Fig. 2) and seedling emergence force of each genotype was calculated.

Experimental design, materials and methods
Seed samples of wheat genotypes were collected from a harvest site at the Queensland Government research farm in Kingsthorpe, Queensland, Australia (27.52°S, 151.79°E). After harvest, seeds were stored in cold temperature (7°C), and before one week of seed germination test, width and length measurement, seeds were warmed to 22°C. For each variety, the thousand-grain weight (TGW, Table 1) was estimated from the weight of 200 seeds from five replicate samples. Using digital slide calipers, the length and width of the seeds were measured for 50 replicate seeds of each genotype (Table 1).
Seedling emergence force of these 16 genotypes were measured in 16 treatment solutions, consisting of four ionic strength (I) values [25, 50, 300 and 484 mmol L À 1, corresponding to EC values of Table 2 Treatments used for Experiment 2 in which the effects of SAR and I on germination and seedling emergence force were investigated for wheat seedling.

Genotype
Total germination (%) Thousand grain weight (TGW, g) Seed width (mm) Seed length (mm) Aurora 91 ( 7 2) 46.1 ( 71.6) 3.82 (7 0.04) 7.71 ( 7 0.11) Axe 98 ( 7 2) 37.5 ( 7 2) 3.38 (7 0.11) 6.52 ( (Fig. 1) to record the force exerted by the seed coleoptile. The device consisted of a stainless steel beam of 0.4 mm thickness and a width of 20 mm suspended above a seed. A strain gauge was attached to the beam to measure the displacement of the beam over time. The wheat seed was placed in a piece of foam that was held underneath the stainless steel beam. A 100 mL container of 1 mM CaCl 2 [ionic strength (I) of 0.003 M L À 1 ] was placed underneath the foam and cotton strings coming out from the foam were placed in the solution. The seeds germinated inside the foam and grew for total 14 d. When the seedling emerged, it pushed and moved the beam upwards. The attached strain gauge measured the movement of the beam from which the emergence force of the seedling was calculated. The strain gauge was connected to a data logger (Quantum X data acquisition system DA, MX840B, HBM, Germany). Measured strain was converted to emergence force (N) using a calibration with strain measured at loads ranging from 0 to 9 N at 1.0 N intervals (Fig. 2).