Dataset on schistosomiasis control using potassium usnate against Biomphalaria glabrata at different developmental stage and Schistosoma mansoni cercariae

This text presents complementary data corresponding to schistosomiasis mansoni׳s vector control and toxicity on Schistosoma mansoni cercariae using potassium usnate. This information support our research article “Potassium Usnate Toxicity Against Embryonic Stages of the Snail Biomphalaria glabrata and Schistosoma mansoni Cercariae” [1], and focuses on the analysis of the detailed data regarding the different concentrations of potassium usnate and their efficiency to B. glabrata mortality and non-viability and S. mansoni cercariae mortality etiologic agent of the disease.

Value of the data The data detail the embryotoxic activity of potassium usnate on the evolutionary stages (blastula, gastrula, trophorora, veliger, and hippo stage) of B. glabrata, correlating the different concentrations applied to the respective stages.
Data report a more detailed view of the malformations and death, express in LC 10 , LC 50 , and LC 90 , of the different stages of development of B. glabrata embryos, of the original article as to their minimum concentration to reach the LC 100 of the embryos.
The data of different times (15, 30, 60, 90 and 120 min) allow us to infer possible time intervals to obtain effective results (LC 10 , LC 50 and LC 90 ) on S. mansoni cercariae.

Data
The data presented in this work provide results related to the unviability, (malformations and death) of embryos in the stages of blastula, gastrula, trophorora, veliger, and hippo stage of the Biomphalaria glabrata after different treatments with the potassium usnate (Table 1), as well as the potassium usnate activity on cercariae of Schistosoma mansoni ( Table 2).

Toxicity assay with S. mansoni cercariae
Cercariae of S. mansoni (Strain -BH) were obtained from of B. glabrata adults (n ¼ 15) previously infected in a laboratory with miracidia (n ¼ 6). After 35 days of infection, the snails were placed in a beaker of 400 mL and submerged in 100 mL of filtered and dechlorinated water and exposed to artificial light (60 W) for 2 h until the cercariae were eliminated to obtain the cercaria suspension. The assay was performed as described in a previous work [5], the estimation of cercariae was calculated by means of an inverted microscope (Leica DM IL Wetzlar, Germany) and an aliquot of 100 cercariae/mL was transferred to a concave glass container and exposed to solutions of the potassium usnate in final concentrations of 100, 10, 5.0, 2.5, 1.5, 1.0 and 0.5 μg/mL. Cercariae from the negative and positive control groups were exposed in filtered and dechlorinated water and to niclosamide at a concentration of 1 μg/mL, respectively. The cercariae were evaluated at intervals of 15, 30, 60, 90 and 120 min after exposure. The following parameters were used for the cercaricidal evaluation: mortality of 10% (LC 10 ), 50% (LC 50 ), and 90% (LC 90 ) at different times after exposure. Two independent experiments were performed in triplicate. Table 2 Lethal concentration (LC) for S. mansoni cercariae exposed to potassium usnate concentration 0.5, 1.0, 1.5, 2.5, 5, 10 and 100 mg/mL during 120 min.