Data on cellular lipids of Yarrowia lipolytica grown on fatty substrates

Yarrowia lipolytica, which is model oleaginous yeast with high industrial interest, was cultivated on fatty substrates. Data concerning fatty acid composition of both substrate and yeast lipids and comparisons of the experimental data with model predictions presented in “Biomodification of fats and oils and scenarios of adding value on renewable fatty materials through microbial fermentations: Modelling and trials with Yarrowia lipolytica” (Vasiliadou et al., 2018) were provided. Furthermore, the total yeast lipids were fractionated into their main fractions, that is, phospholipids, glucolipids plus sphingolipids and neutral lipids, and the fatty acid composition of each lipid fraction was reported.

phospholipids, glucolipids plus sphingolipids and neutral lipids, and the fatty acid composition of each lipid fraction was reported.
& 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Biotechnology, Chemistry More specific subject area Lipid Biotechnology Type of data Tables, figures How data was acquired The yeast Yarrowia lipolytica was cultivated on fatty substrates and the fatty acid composition of both the extracellular and intracellular lipids, as well as of their fractions was determined using an Agilent 7890 A device Gas Chromatography (Agilent Technologies, Shanghai, China).

Data format
Raw samples were collected during growth of Y. lipolytica and processed. Substrate and cellular lipids were purified and analysed.

Experimental factors
Different fatty materials were used as substrates for Y. lipolytica.

Experimental features
Various fats of plant (i.e., olive, sunflower, palm and linseed) and animal (i.e., cod liver and beef tallow) origin were used as carbon substrates for Y. lipolytica. Cultures, carried out in 250-mL Erlenmeyer flasks, were incubated in a rotary shaker (ZHWY211C, Zhicheng, Shanghai, China) at 180 rpm and T ¼287 1°C.

Data source location
University of Patras, Greece Data accessibility The data are available in this article Related research article

Value of the data
The data can be used in order to identify the fatty acid specificity of Yarrowia lipolytica. The composition of lipids (i.e., mainly neutral) accumulated in Y. lipolytica can be pre-determined. New biomodification processes of common fats can be designed.

Data
The data article includes Table 1 reporting fatty acid composition of lipid fractions of Yarrowia lipolytica growing on olive oil, linseed oil, palm oil, sunflower oil, cod liver oil, and beef tallow, and two Figures showing: (1) Experimental data and theoretical predictions of the fatty acid composition of extracellular and intracellular lipids of Y. lipolytica and (2) theoretical fatty acid profiles of the free fatty acid fraction released in the growth medium during growth of Y. lipolytica on the above mentioned fatty substrates.

Experimental design, material, and methods
The yeast Y. lipolytica ACA-DC 50109 was used in the current investigation. The strain was maintained on potato dextrose agar (PDA, Conda, Madrid, Spain) at 7 7 1°C and re-cultured twice a month. Culture conditions: pH 6.0 7 0.5; T ¼ 28°C; agitation rate 280 rpm. Data represent means of two replicates. Experiments were performed in 250-mL Erlenmeyer flasks. The flasks containing 50 71 mL of growth media were sterilized at 121°C for 20 min and thereafter inoculated with 1 mL of a midexponential phase pre-culture containing 4 Â 10 6 cells/mL. The cultures were incubated in a rotary shaker (ZHWY211C, Zhicheng, Shanghai, China) at 180 rpm and T ¼ 28 7 1°C.
Determination of extracellular and intracellular lipids was performed as described in [2]. Intracellular lipids were fractionated as described in [3]. Fatty acid moieties of both extracellular and intracellular lipids and their fractions were converted into fatty acid methyl-esters (FAMEs) and analysed by using a Gas Chromatography (GC; Agilent 7890 A device, Agilent Technologies, Shanghai, China) as described in [4]. Theoretical fatty acid profiles of the free fatty acid fraction released in the growth medium (g/l) vs. time when Yarrowia lipolytica was cultivated on: (i) Olive oil, (ii) linseed oil, (iii) palm oil, (iv) sunflower oil, (v) cod liver oil, and (vi) beef tallow. Culture conditions: As in Table 1. The predictions have been obtained using the mathematical model which is presented in [1]. Experiments were performed in duplicate. Data represent means of two replicates.