Data of antihyperlipidaemic activity for methanolic extract of Tagetes patula Linn. flower head along with piperine, as bioavailability enhancer

The data present in this article is associated with influence of piperine (secondary metabolite) on the antihyperlipidemic and antioxidant activity of methanolic extract of Tagetes patula (METP). METP was evaluated for antihyperlipidemic and antioxidant potential. Phytoconstituents of METP were identified using gas chromatography linked with a mass spectrometer. in vivo antihyperlipidemic activity of METP at the dose of 200 and 400 mg/kg b. wt. and 200 and 400 mg/kg b. wt. along with piperine (20 mg/kg b. wt.) were evaluated by Propylthiouracil induced and Triton X-100 induced hyperlipidemia in rats. Propylthiouracil significantly increased the serum TC (p<0.01), TG (p<0.01), LDL (p<0.01) and VLDL (p<0.01) levels and induction of HDL (p<0.01) at a dose of 400 mg/kg b. wt. along with piperine. Triton X-100 at a single dose of i.p increased lipid levels within 48 h. Increased lipid levels were significantly reduced TC (p<0.01), TG (p<0.01), LDL (p<0.05) and VLDL (p<0.05) by METP at doses of 200 and 400 mg/kg b. wt. along with piperine. Current data were also supported by histological study of livers, Cord pattern of hepatocytes, few periportal lymphocytes in focal area observed in hyperlipidemic rats and hepatocyte, periportal and centrilobular region of liver appear normal in treated group. METP along with piperine (capability to enhance bioavailability and has a property of increasing oral absorption of drugs) showed promising antioxidant and antihyperlipidemic activity which suggests the further use of Tagetes patula extract for the management of hyperlipidemia and atherosclerosis.

with piperine. Current data were also supported by histological study of livers, Cord pattern of hepatocytes, few periportal lymphocytes in focal area observed in hyperlipidemic rats and hepatocyte, periportal and centrilobular region of liver appear normal in treated group. METP along with piperine (capability to enhance bioavailability and has a property of increasing oral absorption of drugs) showed promising antioxidant and antihyperlipidemic activity which suggests the further use of Tagetes patula extract for the management of hyperlipidemia and atherosclerosis. & 2018 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Pharmacy More specific subject area Antihyperlipidemic activity of medicinal plant Type of data Table, text file, graph, figure How data was acquired Gas chromatography and mass spectroscopy was performed on Agilent 6890 series GC-MS instrument with HP-5MS Column (dimensions 30m $ 0.32mm $ 0.25μm) and semi auto analyser.

Data format
Analysed

Experimental factors
Methanol extract of flowers of the Tagetes patula was prepared By soxhlet extract assembly 1. The acute toxicity data for methanol extract of flower heads. 2. Was performed by using female mice followed by OECD guidelines 425. Hyperlipidaemia was induced with propylthiouracil of 10 mg kg-1 b. wt. dosage and 0.01% PTU in drinking water for 7 days. 3. Rats were divided into seven groups of six rats (n ¼6) each. The Group I and II served as normal control and disease control, respectively receives saline (0.2 ml oral). Group III and IV, were treated with METP and Group V and VI were treated with METP along with piperine. The Group VII served as standard Data source location Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad-500090, Telanagana.

Data accessibility
All data are given along with the article and also provided in NCBI repository.

Value of the data
The methods and data can be used to study Tagetes patula for its antihyperlipidaemic property studied in detail.
Comparison of antihyperlipidaemic activity data of METP (200 and 400 mg/kg bd.wt.) alone and along with piperine (20 mg/kg bd.wt) as penetration enhancer also gives reference for researchers for formulation studies.
GC-MS data and in vitro antioxidant activity data of METP also provide valuable reference to compare secondary metabolite and their action as antihyperlipidaemic activity. Furthermore Nutritional ingestion of this plant species will put in innovative scope in the managing of hyperlipidemia and other metabolic disorders.

Data
The present data focuses on antihyperlipidemic capability of Tagetes patula Linn. Tagetes patula Linn. (French marigold) belongs to the family Asteraceae is widely known for its phytochemical and medicinal properties. The data on chemical composition of methanolic extract Tagetes patula Linn was done by gas chromatography and mass spectrometry are shown in Fig. 1 and Table 1. Information regarding changes in lipid profile (TC, TG, LDL, VLDL and HDL) for PUC and triton induced antihyperlipidemic are presented in Table 2 (Fig. 2) and Table 3 (Fig. 3) respectively. Data regarding histological changes of rat hepatocytes of liver are shown in Figs. 4 to 10. The present investigation helps in finding the influence of piperine on antihyperlipidemic activity of Tagetes patula.

Plant collection and extraction
Flower heads of Tagetes patula were procured from plant nurseries in kadiyam, West Godavari district, Andhra Pradesh. Crude material was identified and authenticated by a botanist (Voucher specimen no., TPK-4) from Government Degree College Kukatpally, Hyderabad. The flower heads were dried under shade; coarsely powdered and crude powdered material was used for the extraction process.

Chemicals and reagents
Triton X-100 used was a product of SRL Chemicals, Sisco Research Laboratories PVT LTD. Maharashtra, India. Simvastatin drug used was a product of Sun Pharmaceuticals India LTD., Mumbai, India. Biochemical kits and all other chemicals were of analytical grade.

Plant extract
The powdered crude material of Tagetes patula was extracted with methanol by Soxhletion and crude extract obtained was evaporated to a solid mass, and preserved in desiccators to remove remaining moisture, if present.

Isolation of piperine
Piper nigrum (Black pepper) powder is used for extraction of piperine as per standard methods [1,2].

Identification of phytochemical constituents using gas chromatography
GC-MS analysis was carried out by Agilent 6890 series GC-MS instrument coupled with mass spectroscopy as a detector. The temperature was adjusted to À 30°C to 280/300°C. The HP-5MS column with dimensions 30 m Â 0.32 mm Â 0.25 mm was used for analysis. The oven temperature was adjusted to 35°C and hold time 5 min, ramp 10°C / min up to 220°C. Column flow is 1.2 mL. The inlet temperature was kept at 250°C and the source temperature of 230°C and MS Quard temperature of 150°C (Table 1).

in vitro antioxidant assays
The scavenging ability of free radicals as hydroxyl and NȮwas measured by the method of Kunchandy and Rao (1990) [3,4]. Data outcome is shown in Table 4.       9. Hepatocytes appeared normal, periportal and centrilobular region appeared normal but mild sinusoidal space dilatation noticed in the pessri portal region of liver.  Lipid levels measured using a Cholesterol measurement kit, the data analyzed is presented in Table 3.

Histopathology of the liver of propylthiouracil induced diabetic rats
On 8th days of study, the animals were sacrificed to separate livers, which were fixed in 10% formalin for 24 h and used for histopathological studies. The data of histopathological studies were shown in Figs. 4-10.

Statistical analysis
The results were expressed as mean 7 SEM. The results were subjected to statistical analysis by using one way ANOVA followed by Dunnett's test p o 0.05, p o 0.01 was considered as statistically significant.