Data on B cell phenotypes in baboons with pig artery patch grafts receiving conventional immunosuppressive therapy

This report is related to the research article entitled “B cell phenotypes in baboons with pig artery patch grafts receiving conventional immunosuppressive therapy” (Yamamoto et al., in press). Herein we provide the data regarding pig artery patch xenotransplantation into the baboon׳s aorta, trough levels of tacrolimus and rapamycin in the blood after transplantation, analysis of B cell phenotype on the basis of IgD and CD27 expression in the blood, and analysis of T cell phenotype on the basis of CD28 and CD95 expression in the blood.


Subject area
Medicine, Immunology More specific subject area Transplantation Type of data Value of the data • These data provide methods and analysis of investigating B cells and T cells in xenotransplantation.
• These data describe B cell and T cell monitoring in the pig-to-baboon artery patch model.
• These data provide information on the efficacy of FDA-approved immunosuppressive agents in xenotransplantation.

Analysis of B cell phenotype on the basis of IgD and CD27 expression in the blood of an immunologically-naïve baboon (Fig. 3)
CD22 þ B cell memory phenotypes in the blood were determined on the basis of IgD and CD27 expression by flow cytometry. CD3 À CD22 þ B cells were classified as IgD þ CD27naïve (which express predominantly IgM), IgD þ CD27 þ as non-switched memory (which express predominantly IgM), IgD -CD27 þ as switched memory (which express predominantly IgG), and IgD -CD27as doublenegative (which express both IgM and IgG) (Fig. 3).  Using multicolor flow cytometry, we distinguished CD3 þ CD4 þ or CD3 þ CD8 þ T cells into different subsets on the basis of CD28 and CD95 expression. CD28 þ CD95were classified as naïve cells, CD28 þ CD95 þ as central memory cells, and CD28 -CD95 þ as effector memory cells. (B) Dynamics of repopulating CD4 þ T cell phenotypes after transplantation (Fig. 5B) These data include CD4 þ T cell numbers before immunosuppressive therapy was initiated (control). In B3715, a naïve phenotype persisted. In contrast, B1915 and B15013 showed gradually increasing central memory phenotypes, especially with regard to the percentage of positive cells. In B15013, the increase did not begin until 50 days after transplantation. (C) CD4 þ T cell phenotype in blood and secondary lymphoid tissues (at euthanasia 6 m after transplantation) (Fig. 5C) (LNMNCs ¼ lymph node mononuclear cells; SplMNCs ¼ spleen mononuclear cells.) CD4 þ T cells in B3715 in the blood and secondary lymphoid tissue (SplMNCs and LNMNCs) 6 m after transplantation were mostly of the naïve T cell phenotype. In the other 2 baboons (B1915 and B15013), the CD4 þ T cells in the LNMNCs 6 m after transplantation were mostly of the naïve T cell phenotype; however, in the blood, the CD4 þ T cells were mostly of the central memory T cell phenotype. (D) Dynamics of repopulating CD8 þ T cell phenotypes after transplantation (Fig. 5D) These data include CD8 þ T cell numbers before immunosuppressive therapy was initiated (control). B3715 showed an increasing effector memory phenotype 1 m after transplantation, followed by a gradually recovering naïve phenotype. In contrast, B1915 and B15013 showed immediately increasing effector memory phenotypes, especially with regard to the percentage of positive cells. (E) CD8 þ T cell phenotype in blood and secondary lymphoid tissues (at euthanasia 6 m after transplantation) (Fig. 5E) CD8 þ T cells in B3715 in the blood and secondary lymphoid tissue (SplMNCs and LNMNCs) 6 m after transplantation were mostly of the naïve T cell phenotype. The CD8 þ T cells in B15013 in SplMNCs 6 m after transplantation were mostly of the naïve T cell phenotype. However, the CD8 þ T cells in B1915 and B15013 in the blood and LNMSCs 6 m after transplantation were mostly of the central and effector memory T cell phenotypes.

Pig-to-baboon artery patch xenotransplantation
Details are provided in our research paper [1] and in a previous paper [2].