The data of Escherichia coli strains genes in different types of wastewater

From April 2016 to March 2017, a number of 99 isolates of Escherichia coli were collected from three types of wastewater including urban wastewater (33 isolates), livestock slaughterhouse wastewater (33 isolates) and poultry slaughterhouse wastewater (33 isolate). The specimens were cultured on microbiological media. The bacterial identification was performed by morphological and biochemical tests. Polymerase chain reaction (PCR) method was carried out to detect 2 virulence genes (traT, and fimH) and 4 antibiotic resistance genes (blaTEM, CTX, SHV, and tetA). The data showed that the prevalence rate of traT, fimH,blaCTX, blaTEM,blaSHV, tetA genes were 89.9%, 91.9%, 79.8%, 40.4%, 6.1%, and 91.9%, respectively.


a b s t r a c t
From April 2016 to March 2017, a number of 99 isolates of Escherichia coli were collected from three types of wastewater including urban wastewater (33 isolates), livestock slaughterhouse wastewater (33 isolates) and poultry slaughterhouse wastewater (33 isolate). The specimens were cultured on microbiological media. The bacterial identification was performed by morphological and biochemical tests. Polymerase chain reaction (PCR) method was carried out to detect 2 virulence genes (traT, and fimH) and 4 antibiotic resistance genes (bla TEM, CTX, SHV , and tetA). The data showed that the prevalence rate of traT, fimH,blaCTX, blaTEM,blaSHV, tetA genes were 89.9%, 91.9%, 79.8%, 40.4%, 6

Value of the data
The data can be useful to operators of water and wastewater treatment plants for better microbial contamination control and the need to be aware of the prevalent amount of pathogenic E. coli genes.
The data can be used to show that the prevalence of pathogenic E. coli genes in different environment of urban wastewater, livestock slaughterhouse wastewater and poultry slaughterhouse wastewater are various and treatment of them must be done by different methods.
The gene of E. coli strain isolated from urban wastewater, livestock slaughterhouse wastewater and poultry slaughterhouse wastewater are different, and the prevalence of fimH and tetA genes were much higher than other genes in three types of wastewater.
The data can be used to show that high prevalence of virulence traits was observed in urban wastewater and need to be considered as a health-alarming situation.
The prevalence of antibiotic resistance genes of pathogenic E. coli in urban wastewater was much higher than E.coli bacteria present in livestock slaughterhouse wastewater and poultry slaughterhouse wastewater, respectively.

Sample collection
For the prepared the dataset of this article from April 2016 to March 2017, a number of 99 nonduplicate isolates of Escherichia coli were recovered from three types of wastewater including poultry slaughterhouse wastewater (33 isolates), urban wastewater (33 isolates), and livestock slaughterhouse wastewater (33 isolate) located in Gonabad, Iran.

Bacterial identification
Wastewater samples (250 ml) were collected aseptically in sterile glass bottles [1,2]. Specimens were sent to the clinical microbiology laboratory within 1 h of specimen collection. The collected specimens were cultured on MacConkey and incubated for 24 h at 35°C 7 2 [3,4]. Primary bacterial identification was performed by standard diagnostic tests. Briefly, the overnight pure growth of the organisms on MacConkey agar plates was checked on the basis of Gram staining,colonial morphology and lactose fermentation [5,6]. The isolated colonies were final identified by oxidase, catalase, motility, triple sugar iron agar (TSI) inoculation, citrate utilization, indole, and H 2 S production. The pure bacterial colonies were inoculated onto medium containing 1.5 ml of sterile Tryptic Soy broth (TSB) mixed with glycerol (20%) and stored at À 20°C for further investigation [7,8].

Detection of virulence and resistance genes by polymerase chain reaction (PCR)
For detection of virulence and resistance genes at first the bacterial cells were culture overnight on Mueller-Hinton agar. After than boiling and the PCR methods were used for detection and distribution of virulence genes and antibiotic resistance genes E. coli isolates, Table 2 [9,10].