Data on uncoupling protein-3 levels, hypoxia, low flow ischemia, and insulin stimulation in dystrophin-deficient mdx mouse hearts

The data contain body weights, plasma free fatty acids concentrations and cardiac uncoupling protein-3 protein levels for wild-type and mdx mice. The data provide heart rates, left ventricular contractile functions, coronary flow, phosphocreatine concentrations, and adenosine 5’-triphosphate (ATP) concentrations throughout hypoxia in mdx mouse hearts. This data article also provides left ventricular contractile functions after low flow ischemia with and without glucose, glycogen levels before ischemia or hypoxia, glucose uptake rates during low flow ischemia and insulin stimulation, and insulin-stimulated phospho-Akt protein levels, a protein in insulin signaling, in mdx mouse hearts.


a b s t r a c t
The data contain body weights, plasma free fatty acids concentrations and cardiac uncoupling protein-3 protein levels for wild-type and mdx mice. The data provide heart rates, left ventricular contractile functions, coronary flow, phosphocreatine concentrations, and adenosine 5'-triphosphate (ATP) concentrations throughout hypoxia in mdx mouse hearts. This data article also provides left ventricular contractile functions after low flow ischemia with and without glucose, glycogen levels before ischemia or hypoxia, glucose uptake rates during low flow ischemia and insulin stimulation, and insulin-stimulated phospho-Akt protein levels, a protein in insulin signaling, in mdx mouse hearts.
& The effects of dystrophin deficiency on uncoupling protein-3 levels, glycogen levels, phosphocreatine and ATP concentrations throughout hypoxia, glucose uptake rates during low flow ischemia and insulin stimulation using D-[2-3 H] glucose in mdx mouse hearts were determined. Data source location University of Oxford, United Kingdom Data accessibility All data are available with this article.

Value of the data
This data can be used to further investigate the role of dystrophin in obesity and elevated serum free fatty acids levels in human Duchenne muscular dystrophy patients.
The data can be used to determine whether decreased phosphocreatine and ATP concentrations cause decreased contractile functions after hypoxia or vice verse in mdx mouse hearts.
Glucose uptake rates and phospho-Akt data can be used to investigate the role of dystrophin in signaling.

Data
Mdx mice have abnormal body weights, plasma free fatty acids concentrations and cardiac uncoupling protein-3 protein levels compared with wild-type mice. Mdx mouse hearts have decreased left ventricular contractile functions and faster ATP loss rates during hypoxia compared with wild-types. Mdx mouse hearts also have higher left ventricular end-diastolic pressures and lower glucose uptake rates during low flow ischemia than wild-types. Insulin-stimulated phospho-Akt protein levels are similar in all mouse hearts while mdx mouse hearts have lower glucose uptake rates in response to insulin than wild-types.

Experimental design, materials, and methods
Experiments were carried out using male wild-type (C57BL10) (n ¼ 61) and age-matched mdx (n ¼ 62) mice at 8 months. All the procedures were approved by the Animal Ethics Review Committees, University of Oxford, and by the Home Office, United Kingdom. Plasma free fatty acids concentrations were measured using NEFA kit (Wako Chemicals). Western blot quantified protein levels with anti-uncoupling protein-3, anti-phospho-Akt (Cell Signaling) and anti-GAPDH (Abcam). Isolated whole Langendorff-perfused mouse hearts were equilibrated with fatty acids free Krebs-Henseleit buffer gassed with 95% O 2 -5% CO 2 (PaO 2 ¼ $ 90 mmHg), and subjected to hypoxia with buffer gassed with 95% N 2 -5% CO 2 (PaO 2 ¼ $ 45 mmHg) and reoxygenation. Left ventricular pressures and coronary flow were recorded. Protocols were shown in Fig. 1. Glycogen levels before ischemia or hypoxia were determined [1]. Glucose uptake rates were calculated using D-[2-3 H] glucose during low flow ischemia (0.5 ml min À 1 gww À 1 ) and in response to insulin [2]. Statistical significance was assessed using one-way analysis of variance followed by Student's t-test.

Acknowledgments
Wen Zhang acknowledges Clarendon Scholarships from University of Oxford, United Kingdom, and Hong Kong Oxford Scholarship, United Kingdom, for supporting her DPhil studies.

Transparency document. Supplementary material
Transparency data associated with this article can be found in the online version at https://doi.org/ 10.1016/j.dib.2018.08.010.