Callus and etiolation induction data from explants of Solanecio biafrae (Olive & Hierne) C. Jeffrey cultured in the dark

Different types of explant (leaf, nodal and petiole explant) from in vitro grown plant maintained on Murashige and Skoog (MS) medium, were cultured on MS medium supplemented with various concentrations of 2, 4-Dichlorophenoxy acetic acid(2, 4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/l) in dark condition. Data on callus formation was recorded on 10 days after culture. Number of explants forming callus, callus colour and type were recorded. The plant growth regulator-free media which served as the control induced etiolation resulting in long hypocotyls from the nodal explants.


Subject area
Biology More specific subject area Plant Cell and Tissue culture Type of data

Data accessibility
Data are presented in this article.

Value of the data
The data furnishes scientific body with information on callus induction of Solanecio biafrae using This data is valuable for further study on developing efficient regeneration protocol for S. biafrae.

Data
In the data, effect of 2, 4-Dichlorophenoxy acetic acid on callus formation is shown for three explant types from Solanecio biafrae plant grown in vitro under the dark condition. The age of the explant source for plant tissue culture is significant with preference to younger plants. The age barrier is therefore overcome with the use of in vitro grown plantlets (1). Fig. 1a-c represents the images of callus being formed by different explants type (nodal, petiole and leaf explants). Callus formation was observed on 10 days after culture. Meanwhile, Fig. 2 represents the image of etiolated shoot formation with an average of 4 roots formed from nodal explants cultured on MS basal medium without 2, 4-D (experimental control). Moreover, Tables 1 and 2 show the callogenic and morphogenic responses of S. biafrae explant types to the varied concentrations of 2, 4-D.

Source of primary biological material and disinfection
Plants of S. biafrae were purchased from Lafenwa market in Abeokuta and cultivated via stem cutting in pots under the tree at the College of Science and Technology of Covenant University, Ota, Ogun State. Nodal segments was collected from the potted plants and disinfected under sterile conditions inside a laminar air flow cabinet. The nodal segments were surface sterilized by immersion in 70% ethanol for 5 min, and then immersed in 10% sodium hypochlorite (NaOCl) for 20 min, followed by 5% sodium hypochlorite shaken periodically for 5 min. They were then rinsed several times with sterile distilled water (SDW).  Table 1 Callogenic response of three explants type of S. biafrae on 2, 4-D in dark condition.

In vitro culture of Solanecio biafrae
All the sterilized single node explants were trimmed and cultured on Murashige and Skoog (MS) basal medium [1] supplemented with 3% sucrose. The pH was adjusted to 5.7 using NaOH or HCl before autoclaving at 121°C for 15 min and adding 0.8% agar. All cultures were maintained at 16 hr photoperiod with 3000 lx light intensity at 25 72°C.

Effect of 2,4-D on morphogenesis of S. biafrae
Leaf, nodal and petiole explants were excised from the in vitro grown stock plants maintained on MS basal medium, were cultured on MS medium supplemented with 2, 4-Dichlorophenoxy acetic acid in dark condition to study the morphogenic response of S. biafrae. The treatment with no plant growth regulator was taken as the control and evaluated. Varied concentrations of 2,4-D were added to the media before the pH was adjusted to 5.8 using NaOH or HCl, autoclaved at 121°C for 15 min and 0.7% agar added. The treatments including control (MS basal medium only) are illustrated in Table 3 Media treatments with different concentrations of PGR used for this study.

Treatments
Explants Media