Data for vancomycin elution, activity and impact on mechanical properties when incorporated into orthopedic bone cement

In this article, we report data on the antibiotic elution and efficacy, and mechanical properties of Palacos bone cement with different amounts of added vancomycin (0.0, 0.125, 0.25, 0.5, 1.0, 2.0 g), see “Vancomycin elution, activity and impact on mechanical properties when added to orthopedic bone cement” (Bishop et al., 2018) [1]. Mechanical testing was performed for four-point bending, compression, and fracture toughness. The release characteristics of vancomycin was recorded for up to 60 days to estimate the elution profile. The eluted vancomycin efficacy at eliminating the four most common causative orthopedic implant pathogens is also reported.


Specifications
Value of the data These data are of value in cemented joint arthroplasty using Palacos with added vancomycin as a prophylactic measure against infection.
The mechanical test data of wet cured samples demonstrated that mechanical properties of Palacos bone cement with upto 0.5 g of vancomycin met all ISO minimum requirements.
The release characteristic test data showed that the elution profile is suited for clinical use since the maximum elution occurs during the critical first week after surgery and would effectively eliminate S. aureus contamination that may inadvertently occur during the surgical procedure.
The antimicrobial activity test data showed that the eluted concentration from samples with greater than 0.25 g vancomycin per Palacos packet was sufficient to eliminate a 10 3 colony forming unit per mL (CFU/mL) initial inoculum of S. aureus, including methicillin-resistant S. aureus (MRSA).

Data
The data provided here are Mechanical test data: flexural modulus, flexural strength, compressive modulus, compressive yield strength, and fracture toughness calculated from the force-displacement curves.
Scanning electron microscope (SEM) images from the fracture surfaces of four-point bending samples.
Release characteristic test data for vancomycin added to Palacos bone cement. Antimicrobial activity test data for eluted vancomycin efficacy at eliminating four most common causative orthopedic implant pathogens (MRSA n315, ATTC MRSA 33591, ATCC S.aureus 29213, and ATCC S. epidermidis 35984).

Mechanical testing data
See Tables 1-3. Significantly higher than the ISO minimum requirement of 1800 MPa. 2 Significantly lower than control's bending modulus. 3 Significantly higher than the ISO minimum requirement of 50 MPa. 4 Significantly lower than control's bending strength. Significantly lower than control's compressive modulus. 2 Significantly higher than the ISO minimum requirement of 70 MPa. 3 Significantly lower than control's compressive yield strength.

Drug elution data
See Table 4.

Antimicrobial activity testing
See Tables 5 and 6.

Mechanical testing
After 21 days in 1x PBS at 22°C, mechanical testing was performed (Criterion C43.104, MTS Systems) according to ISO 5388 with force and displacement data recorded at 100 Hz. Displacement rate was 5 mm/min for four-point bending and compression tests and 10 mm/min for fracture toughness tests.

Release characteristic testing
Five cement disks per group, were submerged in 5 mL PBS in an incubator shaker at 37°C and 100 rpm. At 1, 2, 4, 8, 10, 15, 25 and 45, and 60 days, 1.5 mL of the PBS was aspirated off, stored at À 20°C, and samples were submerged in 5 mL fresh PBS. Vancomycin content was measured (high performance liquid chromatography with a C 18 column [3]) in triplicate. Ten microliters of the sample was developed isocratically with 50 mM potassium phosphate buffer (pH 6.8) and acetonitrile (17:3) at a flow rate of 0.5 mL/min. Absorbance was monitored at 210 nm with peak intensity correlated to concentrations [4].