The assembled transcriptome of the adult horn fly, Haematobia irritans

The horn fly, Haematobia irritans irritans (Linnaeus, 1758; Diptera: Muscidae), a hematophagous external parasite of cattle, causes considerable economic losses to the livestock industry worldwide. This pest is mainly controlled with insecticides; however, horn fly populations from several countries have developed resistance to many of the products available for their control. In an attempt to better understand the adult horn fly and the development of resistance in natural populations, we used an Illumina paired-end read HiSeq and GAII approach to determine the transcriptomes of untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males from a Louisiana population of horn flies with a moderate level of pyrethroid resistance. A total of 128,769,829, 127,276,458, 67,653,920, and 64,270,124 quality-filtered Illumina reads were obtained for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. The de novo assemblies using CLC Genomics Workbench 8.0.1 yielded 15,699, 11,961, 2672, 7278 contigs (≥ 200 nt) for untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin + piperonyl butoxide-treated killed adult males, respectively. More than 56% of the assembled contigs of each data set had significant hits in the BlastX (UniProtKB/Swiss-Prot database) (E <0.001). The number of contigs in each data set with InterProScan, GO mapping, Enzyme codes and KEGG pathway annotations were: Untreated Control Adult Females – 10,331, 8770, 2963, 2183; Untreated control adult males – 8392, 7056, 2449, 1765; Permethrin-treated surviving adult males – 1992, 1609, 641, 495; Permethrin + PBO-treated killed adult males – 5561, 4463, 1628, 1211.


Value of the data
Resource for investigations of the molecular basis of insecticide resistance in the horn fly,

Haematobia irritans irritans.
Provides candidate protein coding regions for the development of control strategies targeting adult flies.

Data
RNA was isolated from unfed, newly emerged adult horn flies, including untreated control adult females, untreated control adult males, permethrin-treated surviving adult males and permethrin þ piperonyl butoxide-treated killed adult males. Subsequently, a single lane of 2 Â 54 bp paired end RNASeq reads were obtained, de novo assembled and annotated. The raw reads are accessible at NCBI's SRA through the direct link https://www.ncbi.nlm.nih.gov/sra/SRP131897 or through SRA accession number SRP131897. The adult horn fly transcriptome Shotgun Assembly project has been deposited at DDBJ/EMBL/GenBank under the accession GGLM00000000. The version described in this paper is the first version, GGLM01000000. The overall BioProject ID is PRJNA429442 and the BioSample accessions are SAMN08355023, SAMN08355024, SAMN08355025, and SAMN08355026.

Flies
Adult flies were collected with aerial sweep hand nets from pastured cattle at the St. Gabriel Research Station, Saint Gabriel, Louisiana, and incubated in large inverted Erlenmeyer flasks to collect eggs that were immediately seeded into manure to allow adult fly emergence. The unfed, newly emerged flies were sexed and either immediately frozen at À 80°C for sequencing (females and males) or exposed to low doses of permethrin (1.56 mg/cm 2 , $LD25) or permethrin (1.56 mg/cm 2 , $ LD25) þ 1% piperonyl butoxide (PBO) by the impregnated filter paper assay [1] for 2 h. Adult male flies that survived exposure to permethrin and adult male flies killed by exposure to permethrin þPBO were frozen at À 80°C for sequencing.

RNA isolation
Fourteen unfed, newly emerged adult flies from the untreated control females, untreated control males, permethrin-treated males and permethrin þ PBO-treated males groups were used to purify total RNA in a protocol adapted for use with the FastPrep 24 Tissue and Cell Homogeneizer (MP Biomedicals, Solon, OH, USA) and the FastRNA Pro Green Kit (MP Biomedicals).

Transparency document. Supporting information
Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2018.06.095.