Data on volatile compounds produced by serotype D Clostridium botulinum

We analyzed the volatile compounds produced by serotype D Clostridium botulinum (D-CB16) in trypticase peptone/yeast extract/glucose (TYG) medium using gas chromatography/mass spectrometry (GC/MS). The volatile compounds were captured by solid-phase microextraction and applied to GC/MS for separation and identification of the compounds in TYG medium with or without the cultivation of C. botulinum D-CB16. Thirty-five and 34 volatile compounds were identified in media without and with D-CB16 cultivation, respectively. Of the compounds identified in the medium with the strain, twenty-one were not detected in the original medium, indicating that these were produced by C. botulinum D-CB16.


Subject area
Biology More specific subject area Microbiology Type of data Table  How data was acquired Volatile compounds in the TYG medium with or without cultivation of C. botulinum were analyzed using gas chromatography (GC: 7890 A, Agilent Technologies, Inc.) coupled with mass spectrometry (MS: 5975 C, Agilent Technologies, Inc.).

Data format
Analyzed Experimental factors C. botulinum serotype D strain CB16 was cultured in trypticase peptone /yeast extract/glucose (TYG) medium.

Experimental features
Determined the retention index and identified the volatile compounds and their relative peak areas from the gas chromatograph.

Data source location
Abashiri, Japan Data accessibility Data are presented in this article

Value of the data
This is the first study to provide information on the volatile compounds produced by C. botulinum serotype D strain.
The present data will help in understanding the metabolism of C. botulinum strains. The data will be useful for developing rapid detection methods for C. botulinum strains, which will assist in preventing outbreaks of food-borne botulism and distribution of botulinum strains related to bioterrorism.

Data
The tabular data in this article lists the retention indexes and names of the identified volatile compounds in TYG medium without or with cultivation of Clostridium botulinum serotype D strain CB16, as well as the relative peak area of each compound to that of the internal standard (1 mg/ml 2-octanol) in the gas chromatograph. The retention index and names of the identified compounds, as well as their relative peak areas, are provided in Table 1. The chromatograms obtained from the analyses are shown in Supplementary Figs. 1 and 2.

Design
Serotype D of C. botulinum strain CB16 (D-CB16) was cultured in TYG medium for production of volatile compounds. Volatile compounds were captured from medium both with and without cultivation of the strain.

Materials
C. botulinum D-CB16, obtained from Japanese soil [1], was used in this study. Table 1 Results of GC/MS analysis of volatile compounds collected from TYG medium without (w/o D-CB16) and with (w/ D-CB16) C. botulinum D-CB16 strain. The relative peak areas are indicated as ratios when the area of the internal standard (1 ml/ml 2-octanol) is set as 1.000.

Retention index
Compound Peak area (%)

Volatile compound analysis
Solid-phase microextraction (SPME) was used to identify the volatile compounds in the medium. Volatile compounds in the samples were extracted using a SPME fiber coated with 50/30 mm divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS) (Supelco Co., Bellefonte, PA, USA) with 1-cm standard needle for manual operation Supelco Co., Ref. 57328-U, Bellefonte, PA, USA. Briefly, the SPME fiber was exposed to the headspace of 3,000 mL of each medium at 37°C for 1 h to capture the volatile compounds, following which, it was injected into a gas chromatography (GC) (7890 A, Agilent Technologies Inc., Santa Clara, CA. USA) DB-5 column (60 m Â 0.32 mm i.d., 0.25 μm film thickness; Agilent Technologies Inc.) coupled with mass spectrometry (MS) (5975 C, Agilent Technologies, Inc.) at 220°C for 5 min in splitless mode. The oven temperature was initially held at 60°C and then increased to 230°C at the rate of 3°C/min. Helium was used as the carrier gas at a flow rate of 2.0 mL/min. The temperature of the detector was held at 230°C. Electron impact (EI) mass spectra were recorded at 70 eV in an m/z range of 30-400. The compounds were identified by their GC retention indices, which were calculated from their retention time with respect to those of a series of C 6 -C 18 n-alkanes on a DB-5 capillary column (30 m Â 0.25 mm i.d., 0.25 film thickness; J & W Scientific), and by computer matching using AromaOffice 2D (Nishikawa Keisoku, Tokyo, Japan). The relative amount of each compound was determined from the respective peak area compared to that of 1 ml/ml 2-octanol.