Data on environmentally relevant level of aflatoxin B1-induced human dendritic cells' functional alteration

We assessed the effects of naturally occurring levels of AFB1 on the expression of key immune molecules and function of human monocyte-derived dendritic cells (MDDCs) by cell culture, RT-qPCR, and flow cytometry. Data here revealed that an environmentally relevant level of AFB1 led to remarkably weakened key functional capacity of DCs, up-regulation of key transcripts and DCs apoptosis, down-regulation of key phagocytic element, CD64, and creation of pseudolicensing direction of DCs. Flow cytometry data confirmed a damage towards DCs, i.e., increased apoptosis. The detailed data and their mechanistic effects and the outcome are available in this research article (Mehrzad et al., 2018) [1]. The impaired phagocytosis capacity with triggered pseudolicensing direction of MDDCs caused by AFB1 and dysregulation of the key functional genes could provide a mechanistic explanation for the observed in vivo immunotoxicity associated with this mycotoxin.


a b s t r a c t
We assessed the effects of naturally occurring levels of AFB 1 on the expression of key immune molecules and function of human monocyte-derived dendritic cells (MDDCs) by cell culture, RT-qPCR, and flow cytometry. Data here revealed that an environmentally relevant level of AFB 1 led to remarkably weakened key functional capacity of DCs, up-regulation of key transcripts and DCs apoptosis, down-regulation of key phagocytic element, CD64, and creation of pseudolicensing direction of DCs. Flow cytometry data confirmed a damage towards DCs, i.e., increased apoptosis. The detailed data and their mechanistic effects and the outcome are available in this research article (Mehrzad et al., 2018)  • The novel experimental model is key for further in vitro tests in the area of Immunology, immunobiology, molecular diagnosis and immunotoxicology. • Provision of primary pure MDDCs and their 25 more key genes' sequences can be a road for finding the etiology of various infectious/non-infectious diseases in human. • The immunotoxic aspects of environmental mycotoxins especially AFB 1 and the key functional genes of MDDCs were designed as a model system for others to do further novel experiments in the area of mycotoxins-related infectious/non-infectious diseases, especially cancer.

Data
The analyses of the data on the expression of gene families in MDDCs indicated that the transcript levels of: 1) some key functional gene families, 2) some key TLR-related genes, 3) genes involved in the function of MDDCs and 4) some key cytokine transcripts were altered in post AFB 1 -exposed MDDCs. Further, the flow cytometry-based phagocytosis and apoptosis assay revealed diminished phagocytic and survival capacity of MDDCs. The main finding of data on immune molecules at mRNA levels is briefed in Table 1.
Our data briefly illustrate (Fig. 1) the impaired phagocytosis capacity with triggered pseudolicensing [2] of MDDCs caused by AFB 1 and dysregulation of the key functional genes could provide a mechanistic explanation for the observed in vivo immunotoxicity associated with this mycotoxin [3][4][5][6][7]. Here, we assessed 425 key molecules of AFB 1 -exposed MDDCs at mRNA level with some DCs' key functional assays. Therefore, more in-depth molecular study at protein levels is needed.

Experimental design, materials and methods
Very briefly, experimental work plan were done on pure immature MDDCs with long membrane protrusions; accordingly, the MDDCs were treated with 0 or 10 ng of AFB 1 /ml for 2 and 12 h (37°C, 5% CO 2 , 95% humidity) [3,6] and then used for cellular and molecular analyses [3][4][5][6][7][8]. Key functional gene families, TLR-related genes, genes involved in the function of MDDCs and some key cytokine transcripts were analyzed with qPCR assays [4][5][6]8]. Further the flow cytometry assays were used to quantify phagocytosis and survival capacity of MDDCs [3,6]. All data were as the mean 7SEM of 8 experiments. Table 1 An overview of the effects of AFB 1 on the expression of various gene families and functional alterations in dendritic cells (DCs). The values (changes) are arbitrarily addressed according to the data in this article [1].

Genes
Arbitrary changes

Acknowledgements
The support of research bureaus of Ferdowsi University of Mashhad and University of Tehran is highly appreciated.

Transparency document. Supporting information
Supplementary data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2018.04.104. Fig. 1. Schematic representation from the conceptual interpretation of data of this paper. The scheme depicts how the proposed issue of pseudolicensing of AFB 1 -exposed monocyte-derived dendritic cells (MDDCs) creates/leads to immunodisregulation in vivo. Though mRNA expression of CCR7, CD209 and LFA increase/change, nonetheless the protein levels of those molecules should be evaluated for future work.