Karyomaps of cultured and cryobanked Litoria infrafrenata frog and tadpole cells

These data and analyses support the research article “Culture, cryobanking and passaging of karyotypically validated native Australian amphibian cells” Mollard (2018) [1]. The data and analyses presented here include: (1) three additional karyomaps of cells from the cryobanked and passaged frog and tadpole species Litoria infrafrenata; and (2) combined short-to-long arm ratios of the four karyomaps measured from each respective animal here and in Ref [1].


a b s t r a c t
These data and analyses support the research article "Culture, cryobanking and passaging of karyotypically validated native Australian amphibian cells" Mollard (2018) [1]. The data and analyses presented here include: (1) three additional karyomaps of cells from the cryobanked and passaged frog and tadpole species Litoria infrafrenata; and (2) combined short-to-long arm ratios of the four karyomaps measured from each respective animal here and in Ref [1]. &

Experimental features
Karyotypes of Litoria infrafrenata frog and tadpole cells were determined after culture, freeze-thawing and passaging for expansion. Chromosomes were paired and ordered according to length. Long-to-short arm ratio measurements of each karyotype were compiled to give average measurements for chromosome type designation (e.g. metacentric type).

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Data accessibility Data is with this article

Value of the data
These data are of value to the scientific community for the following reasons: • These data demonstrate reproducibility of karyotypes determined following culture and cryostorage of Litoria infranfrenata tadpole and frog cells, and • These data demonstrate the measured short-to-long arm ratios of each chromosome and provide designation of metacentric, submetacentric and subtelocentric to each chromosome, thus permitting direct comparisons to chromosomal karyomaps from living animals of the same species [1][2][3].

Experimental design, materials and methods
For karyotyping cells were treated for six to eight hours with 0.1 μg/ml KaryoMAX® colcemid (GIBCO) and then stained with 40,60-diamino-2-phenylindole (DAPI; 500 ng/ml; Sigma) according to manufacturer's instructions and as previously described [4]. Slides were prepared by conventional drop-splash technique and coverslipped with DAPI in Gelvatol mounting medium [5]. The largest chromosome was designated chromosome 1, and the remaining were designated following descending chromosomal length [2,3,6]. Chromosome arms were measured using the Levan plugin on Image J software [7]. Chromosomal designation as metacentric, submetacentric or subtelocentric, respectively, were defined as: 1 -1.69, 1.7 -2.99 and 3 -6.99, long arm to short arm ratios, respectively [6]. Imaging was performed under oil immersion at 1000 × using an Olympus BX60 microscope, colour CCD Leica DFC425C camera, and an EL-6000 Leica light source. Photographs of DAPI stained karyotypes were captured using Leica LAS-AF and Q-Capture Pro7 Version 7.0.5 Build 4325 software (QImaging Inc, USA).  Table 1 Chromosome short arm to long arm ratios. Values of short arm to long arm ratios are given as the average of four prepared and measured karyomaps 7 standard deviation. Litoria infrarenata frog and tadpole chromosome 1 is borderline metacentric/ submetocentric for the frog assayed and metacentric for the tadpole assayed, chromosomes 3, 5, 6, 7, 8, 9 and 10 are submetacentric, chromosome 2 is subtelocentric and chromosomes 4, 11 and 12 are metacentric.

Acknowledgements
The author is grateful to Deborah Pergolotti from Frog Safe Inc. (funded through public donations) for providing frog and tadpole tissues from deceased animals. The author is also grateful to Associate Professor Jean-Pierre Scheerlinck and Dr Charlie Pagel for granting access to tissue culture and microscopy facilities at the University of Melbourne's Department of Veterinary and Agricultural Sciences. Richard Mollard provided all funds for the experiments described in this study.

Transparency document. Supporting information
Supplementary data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2018.04.025.