Adsorption of cadmium and lead onto live and dead cell mass of Pseudomonas aeruginosa: A dataset

In this research heavy metals, Cd and Pb, adsorption efficiency was evaluated in aqueous solutions using live and dead biomass of Pseudomonas aeruginosa bacteria. The various important parameters including; pH, temperature, Cd and Pb concentrations, contact time, live and dead cell mass were examined. First, the resistant P. aeruginosa to heavy metals identified and isolated from contaminated soil. Then, the Minimum Inhibitory Concentration (MIC) of Cd and Pb was determined for P. aeruginosa. The highest adsorption efficiency for Cd and Pb were 87% and 98.5%, under dead cell mass of 125 mg, pH 7, temperature 35 °C and contact time 90 min, respectively. The results of this study showed that P. aeruginosa have a high ability to adsorption of Cd and Pb in aqueous solutions.


a b s t r a c t
In this research heavy metals, Cd and Pb, adsorption efficiency was evaluated in aqueous solutions using live and dead biomass of Pseudomonas aeruginosa bacteria. The various important parameters including; pH, temperature, Cd and Pb concentrations, contact time, live and dead cell mass were examined. First, the resistant P. aeruginosa to heavy metals identified and isolated from contaminated soil. Then, the Minimum Inhibitory Concentration (MIC) of Cd and Pb was determined for P. aeruginosa. The highest adsorption efficiency for Cd and Pb were 87% and 98.5%, under dead cell mass of 125 mg, pH 7, temperature 35°C and contact time 90 min, respectively. The results of this study showed that P. aeruginosa have a high ability to adsorption of Cd

Data accessibility
The data are available within this paper.

Value of the data
The data suggest biological adsorption method to removal of heavy metals from soil and aqueous solution.
This data can be used for development of adsorption system for removal of heavy metals from soil, water and wastewater.
This data will be useful for the engineers which is associated with biological purification of water, wastewater and soil refinement.

Data
Environmental pollutants including heavy metals and organic maters has become an issue of severe international concern in recent years [1][2][3][4][5][6][7][8]. The various process can be used for removal of these pollutants from the environment like adsorption systems [9][10][11][12][13][14]. The agricultural soil and groundwater of Guilan Province, north Iran, contains high levels of Cd and Pb. In this work Pseudomonas aeruginosa bacteria isolated from agriculture soil. The various important parameters including; pH, temperature, Cd and Pb concentrations, contact time, live and dead cell mass were examined [14][15][16][17]. The range of Pb and Cd adsorption percentages by using live and dead cell mass of P. aeruginosa are provided in Tables 1-5 at different conditions. The highest adsorption efficiency for Cd and Pb were 87% and 98.5%, under dead cell mass of 125 mg, pH 7, temperature 35°C and contact time 90 min, respectively. The main effects curve for lead and cadmium adsorption by live and dead cell mass of Pseudomonas aerogenosa were provided by Minitab 15 software and were showed in Figs. 1-2.

Materials
All biological and chemical materials used in the experimental, were provided with degree of purity and purchases has been done Merck Company, Germany.

Isolation of Pseudomonas aeruginosa and experiments
First, the agricultural soil samples from Guilan province were prepared at different dilutions and cultured in the cultivation environments nutrient agar and Mac Conkey agar and for 24 h incubated in  For final approval isolated bacteria used from PCR method with characteristics down: Characteristics of primers were presented for P. aeruginosa in Table 6. Also the FTIR of the live and dead cell mass was prepared and presented in Fig. 3.
For live cell mass preparing, after separating the bacteria from the soil, microorganism cultured in Erlenmeyer contain 100 ml lactose broth, incubated for 24 h at temperature 35°C, 200 rpm. Then, it   adsorption process were collected and the efficiency percent of adsorption was calculated by following equation; where c 0 and c t are the concentrations of the primary and secondary heavy metals, respectively. Data analysis was performed using Minitab 15 software.