Lipidomic data on lipid droplet triglyceride remodelling associated with protection of breast cancer cells from lipotoxic stress

The data presented here is related to the research article entitled “Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress” by E. Jarc et al., Biochim. Biophys. Acta 1863 (2018) 247–265. Elevated uptake of unsaturated fatty acids and lipid droplet accumulation are characteristic of aggressive cancer cells and have been associated with the cellular stress response. The present study provides lipidomic data on the triacylglycerol (TAG) and phosphatidylcholine (PC) composition of MDA-MB-231 breast cancer cells exposed to docosahexaenoic acid (DHA; 22:6, ω-3). Datasets provide information on the changes in lipid composition induced by depletion of adipose triglyceride lipase (ATGL) and by exogenous addition of secreted phospholipase A2 (sPLA2) in DHA-treated cells. The presented alterations in lipid composition, mediated by targeting lipid droplet biogenesis and lipolysis, are associated with protection from lipotoxicity and allow further investigation into the role of lipid droplets in the resistance of cancer cells to lipotoxic stress.


a b s t r a c t
The data presented here is related to the research article entitled "Lipid droplets induced by secreted phospholipase A 2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress" by E. Jarc et al., Biochim. Biophys. Acta 1863 (2018) 247-265. Elevated uptake of unsaturated fatty acids and lipid droplet accumulation are characteristic of aggressive cancer cells and have been associated with the cellular stress response. The present study provides lipidomic data on the triacylglycerol (TAG) and phosphatidylcholine (PC) composition of MDA-MB-231 breast cancer cells exposed to docosahexaenoic acid (DHA; 22:6, ω-3). Datasets provide information on the changes in lipid composition induced by depletion of adipose triglyceride lipase (ATGL) and by exogenous addition of secreted phospholipase A 2 (sPLA 2 ) in DHAtreated cells. The presented alterations in lipid composition, mediated by targeting lipid droplet biogenesis and lipolysis, are associated with protection from lipotoxicity and allow further

Value of the data
This lipidomic data provides information on the lipid composition of Ras-driven aggressive MDA-MB-231 breast cancer cells exposed to nutrient stress [1].
It identifies changes in membrane and lipid droplet (LD) composition induced by alterations in LD biogenesis and lipolysis and associated with protection from the lipotoxicity of polyunsaturated fatty acids.
The data describes triacylglycerol (TAG) and phosphatidylcholine (PC) remodelling mediated by human group X secreted phospholipase A 2 (sPLA 2 ) in docosahexaenoic acid (DHA)-treated and adipose triglyceride lipase (ATGL)-depleted breast cancer cells.
The data describes changes in lipid composition following inhibition of TAG lipolysis and LD breakdown by ATGL depletion in DHA-treated breast cancer cells.
The data will foster further examination of the role of LDs in the resistance of cancer cells to nutrient and lipotoxic stress.

Data
In order to determine the changes in TAG composition of LDs and in the profile of cellular PC associated with sPLA 2 -induced protection of breast cancer cells from DHA lipotoxicity, we extracted lipids from MDA-MB-231 cells treated with 100 µM DHA and a combination of 100 µM DHA and 10 nM sPLA 2 and performed lipidomic analyses [1]. The TAG and PC profiles determined are presented in Tables 1 and 2, respectively.
In order to determine the changes in TAG composition of LDs and in the profile of cellular PC in ATGL-depleted breast cancer cells, MDA-MB-231 cells were transfected with ATGL-targeting siRNA or non-targeting scrambled siRNA (SCR). In addition, cells were treated with 10 nM sPLA 2 and 100 µM DHA. Lipids were extracted and lipidomic analyses performed. The data presented includes the Table 1 TAG profiles of control, DHA-and sPLA 2 -treated MDA-MB-231 breast cancer cells. detected TAG (Table 3) and PC species (Table 4) in control (SCR) and ATGL-depleted MDA-MB-231 breast cancer cells (siATGL), either untreated or treated with exogenous DHA, sPLA 2 or both.

Cell culture and treatment
MDA-MB-231 cells were cultured in RPMI-1640 medium in the presence of 10% FBS. Adherent cells were detached using TrypLE Select. Aliquots of stock solutions of DHA in absolute ethanol were stored under argon at −80°C. Prior to addition to cell culture, DHA was incubated in complete medium for 1 h at room temperature. Cells were seeded in complete medium in 6-well plates at 3 × 10 5 cells/well. ATGL silencing was performed by reverse transfection at the time of seeding using a 20 nM mixture of two validated siRNAs targeted at ATGL (Qiagen) or 20 nM Allstars Negative Control siRNA (Qiagen). Transfection complexes were generated using 7.5 µl/well of Lipofectamine RNAiMAX and Opti-MEM Table 2 PC profiles of control, DHA-and sPLA 2    medium. Reverse transfection was performed according to manufacturer's instructions. Cells were left to attach for 24 h and treated with 10 nM sPLA 2 and 100 µM DHA for the following 48 h.

Data handling
The acquired lipidomic data was normalised to total cellular protein. The values are presented as mean percentages of each species per total detected species in each sample with standard errors of the mean (SEM) of three independent experiments.