Data on xylem sap proteins from Mn- and Fe-deficient tomato plants obtained using shotgun proteomics

This article contains consolidated proteomic data obtained from xylem sap collected from tomato plants grown in Fe- and Mn-sufficient control, as well as Fe-deficient and Mn-deficient conditions. Data presented here cover proteins identified and quantified by shotgun proteomics and Progenesis LC-MS analyses: proteins identified with at least two peptides and showing changes statistically significant (ANOVA; p ≤ 0.05) and above a biologically relevant selected threshold (fold ≥ 2) between treatments are listed. The comparison between Fe-deficient, Mn-deficient and control xylem sap samples using a multivariate statistical data analysis (Principal Component Analysis, PCA) is also included. Data included in this article are discussed in depth in the research article entitled “Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by shotgun analyses” [1]. This dataset is made available to support the cited study as well to extend analyses at a later stage.

This article contains consolidated proteomic data obtained from xylem sap collected from tomato plants grown in Fe-and Mnsufficient control, as well as Fe-deficient and Mn-deficient conditions. Data presented here cover proteins identified and quantified by shotgun proteomics and Progenesis LC-MS analyses: proteins identified with at least two peptides and showing changes statistically significant (ANOVA; p r 0.05) and above a biologically relevant selected threshold (fold Z 2) between treatments are listed. The comparison between Fe-deficient, Mn-deficient and control xylem sap samples using a multivariate statistical data analysis (Principal Component Analysis, PCA) is also included. Data included in this article are discussed in depth in the research article entitled "Effects of Fe and Mn deficiencies on the protein profiles of tomato (Solanum lycopersicum) xylem sap as revealed by The MS proteomics data have been deposited to the ProteomeXchange Consortium via the Pride partner repository with the data set identifier PXD007517.

Value of the data
Tomato xylem sap proteins identified and quantified using a shotgun approach are presented herein, providing data on the protein composition of this fluid and facilitating comparisons with other plant species and different plant stresses.
Statistically significant and biologically relevant changes in the xylem sap protein composition upon Fe and Mn deficiencies provide information to assess the effects of these nutritional deficiencies on the metabolic pathways of tomato plants growing in controlled environmental conditions, and results could be compared to those found in other nutritional constraints.
Data would allow other researchers to assess the companion paper [1], extend analyses at a later stage and facilitate the study of target proteins in tomato xylem sap.

Data
The proteome data presented herein were collected from xylem sap fluid of tomato plants grown in two nutritional deficiencies that occur often in plants, Fe and Mn deficiency (Figs. 1 and 2). A shotgun proteomic approach and data processing software were used to identify and quantify a large number of proteins in the xylem sap as well as to assess the changes induced in the proteome of this fluid by these nutritional stresses. The peptides used in the quantification and the protein profiling of the xylem sap proteome are shown in Tables S1 and S2, respectively. To assess the effects of Fedeficiency or Mn-deficiency, the ratios of normalized protein abundances between nutrient-deficient and control samples were calculated, and proteins showing changes statistically significant (ANOVA; p r 0.05) and above a biologically relevant threshold (fold Z 2) are shown in Tables S3 and S4

Experimental design, materials and methods
For each of three treatments (Fe-and Mn-sufficient control, Fe-deficient and Mn-deficient conditions), the xylem sap fluid from six independent batches of plants were collected after eight days of treatment ( Figs. 1 and 2). In each batch of plants, fluid from 16-18 plants was pooled together and considered as a biological replicate (total n ¼ 6). Xylem sap proteins were precipitated, resuspended in the appropriate buffer [1] and analyzed by shotgun proteomics. Mass spectrometry analysis was carried out on an LTQ Orbitrap XL (Thermo Fisher Scientific, Waltham, MA, U.S.A.) equipped with Xcalibur software (v. 2.0.7, Thermo Fisher Scientific). Parameters used were: peptide mass and MS/MS tolerances of 7 5 ppm and 0.6 Da, respectively; one missed cleavage, fixed modification carbamidomethylation (Cys) and variable modification oxidation (Met) allowed; and peptide charges þ 1 to þ 3. Data files obtained were processed (Progenesis QI) and all peptides identified and quantified are shown in Table S1. Proteins identified (MASCOT v. 2.4.1 using the NCBI database) are shown in Table  S2. Data on abundance changes between treatments are provided after using two filters: ANOVA statistical significance (p r 0.05) and a biologically relevant threshold level (fold Z 2) (Tables S3 and  S4, for Fe and Mn deficiencies, respectively). Principal Component Analysis (PCA) analyses were carried out using SPSS Statistical software (v. 24.0) (Fig. 3).
Protein identification was carried out using the list of total peptides with the Mascot search engine [1]. Protein information was exported in Mascot.xml format and imported to Progenesis [1], which then associated peptide and protein information.
Positive protein identification was assigned with at least two unique top-ranking peptides matched and with scores above the statistical and biological threshold levels (ANOVA; p r 0.05 and fold Z 2). Proteins achieving these thresholds are shown in Tables S3 and S4, for Fe-and Mn-deficient samples, respectively.

Transparency document. Supplementary material
Transparency document associated with this article can be found in the online version at http://dx. doi.org/10.1016/j.dib.2018.01.034.