Data describing the flow-mediated vasodilation responses and blood pressure in young adult humans after a single dose of oral edible emu oil

The data provided herein include flow-mediated vasodilation responses, represented by changes in arterial diameter, and blood pressure in young adults after a single oral dose of edible emu oil or placebo (cross-over design). Ten healthy men and 10 healthy women participated. Increased blood flow in the antebrachial region was induced by inflating a pressure cuff and subsequently releasing the pressure by deflating the cuff. After the release, the arterial diameter was continuously monitored for 110 sec using ultrasonic diagnostic equipment. The changes in the arterial diameter from 20 to 110 sec post-cuff deflation are described in line graphs and tables. In addition, systolic and diastolic blood pressure data are provided in a table.


a b s t r a c t
The data provided herein include flow-mediated vasodilation responses, represented by changes in arterial diameter, and blood pressure in young adults after a single oral dose of edible emu oil or placebo (cross-over design). Ten healthy men and 10 healthy women participated. Increased blood flow in the antebrachial region was induced by inflating a pressure cuff and subsequently releasing the pressure by deflating the cuff. After the release, the arterial diameter was continuously monitored for 110 sec using ultrasonic diagnostic equipment. The changes in the arterial diameter from 20 to 110 sec post-cuff deflation are described in line graphs and tables. In addition, systolic and diastolic blood pressure data are provided in a The brachial artery was monitored using ultrasonic diagnostic equipment and the diameter was calculated. In addition, blood pressure was assessed.

Experimental features
A cross-over design was utilized. The emu oil/placebo was administered under conditions of dietary restriction to avoid confounding.

Data source location
Abashiri, Japan Data accessibility The data are supplied with this article

Value of the data
These data are available to determine the effect of emu oil on blood flow-mediated vasodilation responses and blood pressure.
The data presented here may be used as a reference for comparisons with other foods that have an effect on flow-mediated vasodilation responses and blood pressure.
This data offers a valuable and searchable resource on functional foods and can be used for future functional food studies.

Data
Flow-mediated vasodilation is the dilation response of the artery to the shear stress of blood flow, and has been utilized in clinical medicine and functional food studies as a noninvasive method to examine endothelial function [1]. Flow-mediated vasodilation can be represented by the changes in brachial arterial diameter after increased blood flow. To produce the condition of increased bloodflow, pressure can be applied using a pressure cuff and then released by deflating the cuff [2,3]. Figs. 1 and 2 show the changes in the arterial diameter after releasing pressure applied to the antebrachial region via a pressure cuff in emu oil or placebo dosed men and women. Table 1 provides the numeric arterial diameter data used to generate the line graphs in Fig. 1. Blood pressure data are provided in Table 2.

Participants
Twenty healthy students (10 males and 10 females; age range, 19-21 years) from the Hokkaido Okhotsk Campus of the Tokyo University of Agriculture were recruited in July 2017, according to the inclusion and exclusion criteria shown in Supplementary Document 1. The study protocol was approved by the Tokyo University of Agriculture Committee of Human Subject Research Ethics. Informed consent was obtained from all participants.

Materials
Edible emu oil, extracted from the subcutaneous fat of the emu (Dromaius novaehollandiae), was purchased from Tokyo NODAI Bio-Industry (Abashiri, Japan). Fifty milligrams of the oil were encapsulated into soft gelatin capsules (Matsuya, Osaka, Japan). Dose of emu oil was determined based on the amount in blending quantity in the commercially available emu oil capsule, to avoid the unexpected side effect due to emu oil even though the possible side effect has not found in the oil.

Food restriction protocol and flow-mediated vasodilation measurement
The data were collected at the Hokkaido Okhotsk Campus of the Tokyo University of Agriculture between July and August 2017. Using a cross-over design, each participant was randomly administered a single dose of the emu oil or tap water (placebo) in a soft capsule. The second dose of the capsule, which is another sample from single dose, was administered more than a week later. Participants were asked not to take any nutritional supplements on the day before and the morning of the test, with the exception of 90-130 g/meal of cereal (Flugra, Calbee, Tokyo, Japan) and 600-800 mL/day of milk, to meet Dietary Reference Intakes for Japanese 2015 from Ministry of Health, Labour and Welfere of Japan. Flow-mediated vasodilation was measured 3 h after the oil was administered. Precise criteria for the measurement based on the Guidelines for non-invasive vascular function test [4] is described in Supplementary document 2. To arrest the blood flow in the brachial artery, pressure (blood pressure plus 40 mmHg) was applied to the antebrachial region for 5 min using the cuff of a mercurial sphygmomanometer (Yamasu, Japan). Avascularization was monitored by using color Doppler option of ultrasonic diagnostic equipment (LOGIQ P6, GE Healthcare, Little Chalfont, UK). The pressure was released by deflating the cuff, and the brachial artery was monitored continuously using LOGIQ P6. Electro-cardiogram was concomitantly monitored to detect diastolic potential. Arterial diameter (AD) when the maximum diastolic potential was measured using the custom-designed edge-detection software FMDscope (Media Cross Co. Ltd, Japan). The AD was determined based on average of 10times measurements. Potential error in measurements was detected by using trend graph of AD, and omitted from the calculation of average score. Blood pressure was measured using an electronic sphygmomanometer (ES-P2000, Terumo, Tokyo, Japan). We confirmed that no significant side effect was observed from any participant in this study.

Funding
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.