Complete genome sequence of native Bacillus cereus strains isolated from intestinal tract of the crab Ucides sp.

Bacillus cereus is a gram positive bacterium with sporulation capacity. Here, we report the complete genome sequence of two native B. cereus strains (#25 and #29) isolated from intestinal tract of the crab Ucides sp. from Pacoti River in the State of Ceará, Brazil. The findings of this study might increase the molecular information for Bacillus strains. The data can be used in comparative analyses, origin and distribution, as well support for genetic engineering.


a b s t r a c t
Bacillus cereus is a gram positive bacterium with sporulation capacity. Here, we report the complete genome sequence of two native B. cereus strains (#25 and #29) isolated from intestinal tract of the crab Ucides sp. from Pacoti River in the State of Ceará, Brazil. The findings of this study might increase the molecular information for Bacillus strains. The data can be used in comparative analyses, origin and distribution, as well support for genetic engineering.  Value of the data • The data of the present study might increase the molecular information for B. cereus strains, isolated from crab in saline environment. The genomic data can be used in the development of other researchers such as comparative analyses using genes and genomes, origin and distribution of strains. • Our data can give support for genetic engineering of B. cereus strains.
• This work can offer contributions for the molecular diversity of the B. cereus strains improving the pathogenicity assessment or probiotic application in the production of marine organisms.

Data
In the present work, we describe the whole-genome shotgun (WGS) analysis of two strains of B. cereus (Fig. 1)

Isolation of native Bacillus strains
The native strains #25 and #29 were isolated from intestinal tract of the crab Ucides sp., from Pacoti River in the State of Ceará, Brazil. The intestinal tracts were homogenized with saline solution   (NaCl 0.85%) and incubated at 70°C for 1 h. An inoculum was plated on solid medium (NaCl 1.5%) and maintained at 30°C for 48 h. The colonies similar with Bacillus were inoculated in Tryptone Soy Agar (TSA) and the culture integrity was evaluated with Gram Staining by microscopy. The gram positive Bacillus with sporulation capacity were destined to the Laboratory of Structural Genomics of the Federal University of Pelotas (UFPel, Brazil), for whole genome sequencing.

Isolation of genomic DNA
The strains were cultivated in Luria-Bertani (LB) medium at 37°C with vigorous shaking (250 rpm) for 12 hours. The integrity was accessed by microscopy using Gram Staining method. Scanning Electron Microscope (JEOL JSM -6610 LV) was performed using the pellet from culture washed twice with ultrapure water and quickly fixed in Bunsen flame. Genomic DNA was obtained using Illustra Bacteria GenomicPrep Mini Spin kit (GE Healthcare, USA), according the manufacturers guidelines.

Library preparation and sequencing
Bacterial genome sequencing was performed using the Ion Torrent PGM (Life Technologies, Saint Aubin, France) on 100 ng of DNA. The DNA library was constructed using enzymatic fragmentation and adaptor ligation with the Ion Xpress Plus fragment library kit (Life Technologies). Fragment size selection was performed using E-Gel® SizeSelect 2% (Invitrogen). After diluting the library at 100 pM, template preparation, emulsion PCR, and ion sphere particle (ISP) enrichment were performed using the Ion One Touch template kit (Life Technologies). The ISPs were loaded and sequenced on a 318 chip (Life Technologies).

Genome assembly
Raw sequence reads were obtained from the Ion Torrent server in BAM format and converted to FASTQ using the bamToFastq program from the BedTools package [1]. Reads with more than 20% of the bases with Phred score (Q) smaller than 20 where removed using the fastq_quality_filter script from the fastx-toolkit package (http://hannonlab.cshl.edu/fastx_toolkit/). De novo genome assemblies were generated for each strain using MIRA (http://mira-assembler.sourceforge.net/), SPAdes [2] and Newbler (http://www.roche.com/), and for each strain a consensus assembly was generated using CISA [3]. The final set of contigs from each strain was ordered with the program CAR [4], which is able to identify genome inversions and transpositions, using the genome of Bacillus cereus strain 03BB102 (GenBank: CP009318.1) as reference. The genome annotation was performed using Genix [5], and manually revised using Artemis [6].
A BLAST search [7] was performed against the NCBI nucleotide database (https://www.ncbi.nlm. nih.gov/BLAST/) to identify the most similar strain. Chromosome features of Bacillus cereus strains were drawing using DNAPlotter [8].