Two-dimensional NMR data of a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans and schizophyllan from Schizophyllum commune

This article contains two-dimensional (2D) NMR experimental data, obtained by the Bruker BioSpin 500 MHz NMR spectrometer (Germany) which can used for the determination of primary structures of schizophyllan from Schizophyllum commune (SPG) and a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans. Data include analyzed the 2D NMR spectra of these β-glucans, which are related to the subject of an article in Carbohydrate Polymers, entitled “NMR spectroscopic structural characterization of a water-soluble β-(1→3, 1→6)-glucan from A. pullulans” (Kono et al., 2017) [1]. Data can help to assign the 1H and 13C chemical shifts of the structurally complex polysaccharides.


a b s t r a c t
This article contains two-dimensional (2D) NMR experimental data, obtained by the Bruker BioSpin 500 MHz NMR spectrometer (Germany) which can used for the determination of primary structures of schizophyllan from Schizophyllum commune (SPG) and a water-soluble β-(1→3, 1→6)-glucan from Aureobasidium pullulans. Data include analyzed the 2D NMR spectra of these β-glucans, which are related to the subject of an article in Carbohydrate Polymers, entitled "NMR spectroscopic structural characterization of a water-soluble β-(1→3, 1→6)-glucan from

Data source location
National Institute of Technology, Tomakomai College, Nishikioka 443, Tomakomai, Hokkaido 059 1275, Japan Data accessibility Data are with this article.

Value of the data
The following data detail NMR characterization of a novel water-soluble β-(1→3, 1→6)-glucan and schizophyllan from Schizophyllum commune.
The NMR data can be helpful to estimate the branching patterns of other β-glucans. NMR parameters for the data can be useful for structural characterization of complex polysaccharides.

Experimental design, materials and methods
The experiment's planning, design, and data processing correspond to the protocol given in Refs. [1,2].  Fig. 1) are denoted by solid red, solid and dashed blue, and solid green lines, respectively. 1 H and 13 C NMR spectra of SPG are shown in horizontal and vertical axes in the HSQC spectrum, respectively, and the 1 H and 13 C resonance assignments are indicated in the 1 H and 13 C spectra.

Description of the NMR experiments
Each sample was dissolved in 600 μL of DMSO-d 6 (99.9% isotropic purity, Sigma-Aldrich (USA)). All NMR spectra were recorded on a Bruker AVIII 500 MHz spectrometer at 363 K. HSQC data were acquired on a 2048 × 256-point matrix for the full spectrum, with 96 scans per increment, and the interpulse delay which corresponded to 1/4 J CH was set to 3.44 ms. HMBC) data were acquired on a 1024 × 256-point matrix for the full spectrum, with 128 scans per increment, and the delay time for the evolution was set to 62.5 ms. ROESY data were acquired on a 2048 × 256-point matrix for the full spectrum with 64 scans per increment, and the mixing time was 200 ms. The repetition time of each 2D NMR experiment was 2 s, and all 2D NMR data were zero-filled to 2k in both dimensions prior to Fourier transformation. 1 H and 13 C chemical shifts were calibrated using the methyl resonances of DMSO at 2.52 ppm for 1 H and 39.52 ppm for 13 C.