S-nitroso- and nitro- proteomes in the olive (Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS

The data presented here are related to the research article entitled “Generation of nitric oxide by olive (Olea europaea L.) pollen during in vitro germination and assessment of the S-nitroso- and nitro-proteomes by computational predictive methods” doi:10.1016/j.niox.2017.06.005 (Jimenez-Quesada et al., 2017) [1]. Predicted cysteine S-nitrosylation and Tyr-nitration sites in proteins derived from a de novo assembled and annotated pollen transcriptome from olive tree (Olea europaea L.) were obtained after using well-established predictive tools in silico. Predictions were performed using both default and highly restrictive thresholds. Numerous gene products identified with these characteristics are listed here. An experimental validation of the data, consisting in nano-LC-MS (Liquid Chromatography-Mass Spectrometry) determination of olive pollen proteins after immunoprecipitation with antibodies to anti-S-nitrosoCys and anti-3-NT (NitroTyrosine) allowed identification of numerous proteins subjected to these two post-translational modifications, which are listed here together with information regarding their cross-presence among the predictions.

. Predicted cysteine S-nitrosylation and Tyr-nitration sites in proteins derived from a de novo assembled and annotated pollen transcriptome from olive tree (Olea europaea L.) were obtained after using well-established predictive tools in silico. Predictions were performed using both default and highly restrictive thresholds. Numerous gene products identified with these characteristics are listed here. An experimental validation of the data, consisting in nano-LC-MS (Liquid Chromatography-Mass Spectrometry) determination of olive pollen proteins after immunoprecipitation with antibodies to anti-S-nitrosoCys and anti-3-NT (NitroTyrosine) allowed identification of numerous proteins subjected to these

Subject area Biology
More specific subject area

NO signaling in plant tissues
Type of data Tables  How data was  acquired Predictive software, nano-liquid chromatography-mass spectroscopy

Value of the data
Obtained data can be compared with dataset obtained from other sources or predictions, enabling for example comparisons between vegetative/reproductive tissues, diploid/haploid material or signaling capabilities throughout differential metabolic pathways.
nano-LC-MS determinations allowed building a list of structural, enzymatic, regulatory etc. proteins that should be further assessed regarding whether these gene products are really modified by NO in vivo, determining under what conditions they can be modified, the effects of such modifications, reversibility of the modifications (S-nitrosylation), and many other aspects.
Information listed here can be used to improve performance of the predictive tools used here and to create new programs: algorithms are usually modified after feedback from experimental studies. Proteins listed here after MS identification and non-predicted by the in silico tools are a good start point for such improvements.

Data
Predicted S-nitrosoproteome of olive pollen is provided through 3 different tables (Table 1- Tables 4-5 provide information regarding predicted Tyr-nitroproteome of olive pollen, which include the use of a predictive tool under two different restrictive conditions for the prediction. Valuable additional information is also included, like Protein id in ReprOlive database, Tyr-nitration position/s, S-nitrosylation position/s, FLN ortholog, FLN status and FLN definition and TAIR database ortholog. Experimentally-determined S-nitrosoproteome of olive pollen is provided in the form of a table (Table 6) with information about the peptides identified, annotation details and searches of these proteins throughout the tools for prediction of S-nitrosylation and Tyr-nitration.
Experimentally-determined Tyr-nitroproteome of olive pollen is provided in the form of a table (Table 7) with information about the peptides identified, annotation details and searches of these proteins throughout the tools for prediction of S-nitrosylation and Tyr-nitration.

Immunoprecipitation and nano LC-MS analysis
Proteins from 'Picual' mature pollen homogenates were subjected to immunoprecipitation with either an anti-3-NT polyclonal (Millipore) or anti-S-nitrosoCys polyclonal (Sigma-Aldrich) antibodies conjugated to protein A agarose beads (Sigma-Aldrich), which were then collected by centrifugation and washed. Samples were subjected to SDS-PAGE gels. Bands of interest were excised after colloidal Coomassie staining of gel, and subjected to nano LC-MS analysis using an NanoAcquity nano-HPLC (Waters), equipped with a Waters BEH C18 nano-column (LC step). Mass spectrometry was performed in a Synapt G2Si ESI Q-Mobility-TOF spectrometer (Waters) equipped with an ion mobility chamber (T-Wave-IMS). Database searching was performed using MASCOT 2.2.07 (Matrixscience, London, UK) against an ad hoc in house made database corresponding to Olea europaea L [1,5].

Transparency document. Supplementary material
Transparency data associated with this article can be found in the online version at http://dx.doi. org/10.1016/j.dib.2017.09.058.