Data on efficacy of umbelliferone on glycoconjugates and immunological marker in 7,12-dimethylbenz(a)anthracene induced oral carcinogenesis

Umbelliferone, a phenolic coumarin and dietary agent is believed to play a key role in pharmacological activities including anti-cancer and anti-oxidants effect in various in vitro and in vivo models. In present data on the pre-treatment of umbelliferone (30 mg/kg b.w.) for 16 weeks to 7,12-dimethylbenz(a)anthracene induced hamsters provides protection on cellular integrity by observing the status of cell surface glycoconjugates in the circulation and buccal mucosa and cytokeratin immunoexpression in the buccal mucosa of experimental animals. Data presented in this article brief that umbelliferone exhibits potent to clear cell surface abnormalities in buccal tissues and circulation during carcinogenesis and restored the expression of cytokeratin effect against 7,12-dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis, which is attributes to its inhibitory role on glycoprotein synthesis or on the activity of the glycosyltransferase. In an article associates with this data set given the relevance to the research article entitled “Dose responsive efficacy of umbelliferone on lipid peroxidation, anti-oxidant, and xenobiotic metabolism in 7,12-dimethylbenz(a)anthracene-induced oral carcinogenesis” namely Vijayalakshmi and Sindhu, 2017 assessed 100% tumour formation in 7,12-dimethylbenz(a)anthracene treated hamsters and oral administration of umbelliferone at a dose of 30 mg/kg b.w to 7,12-dimethylbenz(a)anthracene treated hamsters prevents tumour incidence, restores the status of the biochemical markers in circulation and buccal mucosa and also dysregulation in the expression of molecular markers. Given the relevance to this article entitled “Berberine protects cellular integrity during 7,12-dimethylbenz[a]anthracene-induced oral carcinogenesis in golden Syrian hamsters” namely Sindhu and Manoharan 2010, which were based on spectrophotometry and florescence microscope analysis.


a b s t r a c t
Umbelliferone, a phenolic coumarin and dietary agent is believed to play a key role in pharmacological activities including anticancer and anti-oxidants effect in various in vitro and in vivo models. In present data on the pre-treatment of umbelliferone (30 mg/kg b.w.) for 16 weeks to 7,12-dimethylbenz(a)anthracene induced hamsters provides protection on cellular integrity by observing the status of cell surface glycoconjugates in the circulation and buccal mucosa and cytokeratin immunoexpression in the buccal mucosa of experimental animals. Data presented in this article brief that umbelliferone exhibits potent to clear cell surface abnormalities in buccal tissues and circulation during carcinogenesis and restored the expression of cytokeratin effect against 7,12-dimethylbenz(a)anthracene induced hamster buccal pouch carcinogenesis, which is attributes to its inhibitory role on glycoprotein synthesis or on the activity of the glycosyltransferase. In an article associates with this data set given the relevance to the research article entitled "Dose responsive efficacy of umbelliferone on lipid peroxidation, anti-oxidant, and xenobiotic metabolism in 7,12-dimethylbenz(a)anthracene-induced oral carcinogenesis" namely Vijayalakshmi and Sindhu, 2017 assessed 100% tumour formation in 7,12-dimethylbenz(a)anthracene treated hamsters and oral administration of umbelliferone at a dose of 30 mg/kg b.w to 7,12-dimethylbenz(a)anthracene treated hamsters prevents Contents lists available at ScienceDirect journal homepage: www.elsevier.com/locate/dib tumour incidence, restores the status of the biochemical markers in circulation and buccal mucosa and also dysregulation in the expression of molecular markers. Given the relevance to this article entitled "Berberine protects cellular integrity during 7,12dimethylbenz[a]anthracene-induced oral carcinogenesis in golden Syrian hamsters" namely Sindhu and Manoharan 2010, which were based on spectrophotometry and florescence microscope analysis.
& 2017 Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Subject area
Oral cancer biology.

Most specific subject area
Oral biology, oral oncology, cell biology, cancer biology, histology and biochemistry.

Types of data
Figures How the data were acquired Spectrophotometer and fluorescence microscope (Nikon eclipses TS100).

Data format
Raw analyzed data. Experimental factors Biochemical analysis was determined in circulation and buccal tissue. Buccal tissue glycoproteins were assessed using spectrophotometry. Cytokeratin immunoexpression patterns were observed by using fluorescence microscope Experimental features Hamsters, 80-120 g, procured from the National Institute of Nutrition, India and were maintained in the central animal house, in polypropylene cages, room temperature (277 2°C), humidity 55°C. Data source location Annamalai University, Department of Biochemistry and Biotechnology, Annamalainagar, India. Ramachandra University, Department of Oral pathology, Chennai.

Data accessibility
All data are provided with this article.
Value of the data • Data will be useful to understand the effect of umbelliferone on the biochemical and cellular changes in hamsters buccal mucosa. • Umbelliferone mediated modulation of glucoproteins expression could be further investigated to find its role in signalling mechanism during neoplastic transformation. • Data from this study using 7,12-dimethylbenz (a) anthracene induced hamster buccal pouch carcinogenesis will provide a standard model to test the chemotherapeutics and preventives. • Present data described the pattern of glycoconjugate expression using biochemical, periodic acid sciffs staining and immunohistochemistry in the buccal mucosa and highlighted increased glycoconjugate expression, severe keratosis, hyperplasia, dysplasia and well differentiated squamous cell carcinoma, over expression of cytokeratin in tumor bearing hamsters. • The data can be used to link the membrane stabilizing effects of umbelliferone on cell surface glycoconjugate expression oral carcinogenesis.

Data
Detection of glycoconjugates and cytokeratin expression could help to assess the cancer progression. The protective role of umbelliferone during oral carcinogenesis via the inhibitory role on an abnormal glycoprotein synthesis or regulatory glycosyl transferase activity which was observed through the restored expression of cytokeratins, in experimental animals. Histopathological and immunohistochemical findings of the present study indicates that membrane stabilising effect exerted by umbelliferone during 7,12-dimethylbenz(a)anthracene induced experimental carcinogenesis.

Experimental design
A totally 24 hamsters were randomized into four groups (n ¼6). Tumour was induced in the left buccal pouch of hamster by topical application of 0.5% 7,12-dimethylbenz (a) anthracene in liquid paraffin thrice a week for 14 weeks. Fig. 1A shows umbelliferone (30 mg/kg b.w., dissolve in 10% dimethyl sulfoxide, p.o.,) three times a week on days alternative to 7,12-dimethylbenz(a)anthracene application, starting a week before the exposure to the carcinogen and continued throughout the experimental period. Fig. 1B was showed the experimental design. At 16th week, hamsters were euthanized by cervical dislocation, the buccal pouches were everted, and the degree of lesions were inspected to evaluate premalignant lesions and tumor development (Fig. 4A).

Biochemical studies
The protein bound hexose and total sialic acid in plasma and defatted buccal tissue was estimated by the method of Niebes [3] and Dische [5] respectively. The protein bound hexosamine in plasma was estimated by the method of Wagner [4] Figs. 2 and 3.

Histological analysis
Tumour specimens were fixed in 10% (v/v) neutral formalin solution for 24 h and processed routinely by embedding in paraffin. Tissue serial sections (2-3 µm) were immersed with 0.1% periodic acid staining for 15 min, at 50°C. The slides were immersed in schiff's reagent for 40 min, subsequently counterstained with hematoxylin, dehydrated in graded ethanol, cleared in xylene and examined under the light microscope (Nickon eclipse TS100, Japan) Fig. 4B.

Immunohistochemical analysis for cytokeratin expression
Immunohistochemical analysis for cytokeratin was performed according to the standard avidinbiotin complex method described in procedure programme of biotinylated rabit anti-mouse antibody and an avidin-biotin complex (Biogenesis, India). A previously known positive tumour tissues was used as a positive control for cytokeratin. The immunostaining grades were quantitatively analyzed by biological image analysis systems which consist of Nickon TS100 (Fig. 4C).

Statistical analysis
Data were expressed as mean 7S.D. ANOVA and Student's t-test were used for determining differences between groups, and P r0.05 was regarded as statistically significant.

Ethical approval
The animal treatment and protocol employed was approved by the Institutional Animal Ethics Committee (Registration Number 160/1999/CPCSEA; Proposal No. 1092: dated 09.10.2014), Annamalai University. The animals were kept in compliance with the "Guide for the care and use of laboratory animals" and committee for the purpose of control and supervision on experimental animals.