Data of PCL-b-P(MMA-DMAEMA)2 characterization and related assays

The data presented here are related to the research paper entitled “PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors” by Franco et al. [1]. Characterization data of PCL-diol, macroinitiator Br-PCL-Br, homopolymers (PMMA and PDMAEMA) and copolymers (batch 1 and batch 2) analyzed by FTIR, SEC and NMR, as well as, characterization of PCL-NS formulation by laser diffraction and DLS analysis, initial nanocapsule formulations and 1C-NC and 2C-NC formulations, including hydrodynamic diameter at different pH media, and DMSO cytotoxicity.


Specifications
Area Chemistry, Biology, Pharmacy More specific subject area

Copolymer synthesis and pH-sensitive nanocapsules
Type of data Tables and figures How data was acquired Synthesis and products isolation by filtration and purification by impurities dissolution. Nanocapsules were analyzed as produced, without pre-treatment Experimental features Chemical characterization and identification of modifications induced by synthesis procedures or by formulation of materials Data source location Commercial reagent: PCL, MMA and DMAEMA

Data accessibility
Data is provided with this article

Value of data
Characterization spectra of the materials were compared with data from other works when developing a similar delivery system or copolymer synthesis.
SEC and NMR data provided information on the efficiency of the copolymer synthesis and were useful for their identification.
Nanocapsules parameters and it response to different pH media is innovative for scientific community since the copolymer maintains its integrity and expands upon acid pH.
The bromide end-group of the copolymer permit application as active targeting system after covalent binding with ligands.

Data
The data presented in Section 1.1 is the 1 H NMR analysis of the homopolymers PMMA and PDMAEMA (Fig. 1). Section 1.2 involves the profiles by laser diffraction of PCL-NS and its parameters (Fig. 2, Table 1). The data presented in Section 1.3 includes the synthesis of the macroinitiator and the characterization by FTIR and SEC analysis of the PCL-diol and Br-PCL-Br (Fig. 3), 1 H NMR (Fig. 4) and 13 C NMR (Fig. 5). Section 1.4 brings data referent to the copolymers (batch 1 and batch 2) with FTIR, SEC ( Fig. 6), 1 H NMR (Fig. 7) and 13 C NMR (Fig. 8). The data contained in Section 1.5 is related to the characterization of nanocapsules formulations, as size distribution profiles of initial nanocapsule formulations ( Fig. 9) and 1C-NC and 2C-NC formulations (Fig. 10), including its parameters (Table 2) and the DLS profile ( Fig. 11) and it behavior in different pH (Fig. 12). Section 1.6 presented the DMSO cytotoxicity data (Fig. 13).

Experimental design, materials and methods
The methodologies to obtain the data exposed here are described in [1] and in cited references. PCL-NS was prepared using PCL (14,000 g mol -1 , 0.0301 g) solubilized in 30 mL of acetone:ethanol 1:1 (v/v) and injected into an aqueous dispersion (60 mL) of polysorbate 80 (0.0518 g), having its volume reduced to 10 mL.

Table 3
Nanocapsules initial formulations and formulations.

Materials and quantities a
Initial formulation anisole) was added by a syringe at once. After 2 min, a solution of Tin (II) 2-ethylhexanoate (32 mg, 0.08 mmol) in anisole (2 mL) was drop-wised using a syringe. Then, the temperature was raised to 90°C and the reaction was maintained under stirring for 24 hours. After cooling to room temperature, THF (5 mL) was added and the copolymer was precipitated in cold cyclohexane (50 mL). To remove the catalyst and non-reacted monomers, the crude solid was dissolved in 2-propanol (5 mL) and precipitated in cold cyclohexane (50 mL). The precipitate was isolated by filtration and dried under vacuum (Edwards Weg ® C56 0698, Brazil).
Initial nanocapsule formulations and 1C-NC and 2C-NC formulations are described in the Table 3.